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Field | Value |
---|---|
Namespace | Molecular function |
Short description | Methylthiotransferase activity |
Full defintion | Catalysis of the addition of a methylthioether group (-SCH3) to a nucleic acid or protein acceptor. |
Subterm of |
The relationship of GO:0035596 with other GO terms.
Relationship type | GO terms |
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Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0035596, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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GOC | jh2 |
PMID | Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis. Nucleic Acids Res. 2010 Oct; 38 (18): 6195–205.PMID: 20472640 Methylthiotransferases (MTTases) are a closely related family of proteins that perform both radical-S-adenosylmethionine (SAM) mediated sulfur insertion and SAM-dependent methylation to modify nucleic acid or protein targets with a methyl thioether group (-SCH(3)). Members of two of the four known subgroups of MTTases have been characterized, typified by MiaB, which modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA, and RimO, which modifies a specific aspartate residue in ribosomal protein S12. In this work, we have characterized the two MTTases encoded by Bacillus subtilis 168 and find that, consistent with bioinformatic predictions, ymcB is required for ms(2)i(6)A formation (MiaB activity), and yqeV is required for modification of N(6)-threonylcarbamoyladenosine (t(6)A) to 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A) in tRNA. The enzyme responsible for the latter activity belongs to a third MTTase subgroup, no member of which has previously been characterized. We performed domain-swapping experiments between YmcB and YqeV to narrow down the protein domain(s) responsible for distinguishing i(6)A from t(6)A and found that the C-terminal TRAM domain, putatively involved with RNA binding, is likely not involved with this discrimination. Finally, we performed a computational analysis to identify candidate residues outside the TRAM domain that may be involved with substrate recognition. These residues represent interesting targets for further analysis. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: CDK5RAP1-like protein-like [Glycine max] gi|356540793|ref|XP_003538869.1| | 2 | ||
– | PREDICTED: CDKAL1-like protein-like [Glycine max] gi|356542899|ref|XP_003539902.1| | 2 | ||
– | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT4G36390.1 Methylthiotransferase; Swiss-Prot: sp|Q8H0V1|CK5P1_ARATH CDK5RAP1-like protein; TrEMBL-Plants: tr|G7KGB3|G7KGB3_MEDTR Radical SAM methylthiotransferase, MiaB/RimO family protein; Found in the gene: LotjaGi2g1v0385400 | 2 | ||
– | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT4G36390.1 Methylthiotransferase; Swiss-Prot: sp|Q2R1U4|CK5P1_ORYSJ CDK5RAP1-like protein; TrEMBL-Plants: tr|K7LNH5|K7LNH5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0385400 | 2 | ||
– | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT4G36390.1 Methylthiotransferase; Swiss-Prot: sp|Q2R1U4|CK5P1_ORYSJ CDK5RAP1-like protein; TrEMBL-Plants: tr|K7LNH5|K7LNH5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0385400 | 2 | ||
– | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT4G36390.1 Methylthiotransferase; Swiss-Prot: sp|Q2R1U4|CK5P1_ORYSJ CDK5RAP1-like protein; TrEMBL-Plants: tr|K7LNH5|K7LNH5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0385400 | 2 | ||
– | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT1G72090.1 Methylthiotransferase; Swiss-Prot: sp|Q6P4Y0|CDKAL_XENTR Threonylcarbamoyladenosine tRNA methylthiotransferase; TrEMBL-Plants: tr|I1LNN6|I1LNN6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0556600 | 2 | ||
– | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT1G72090.1 Methylthiotransferase; Swiss-Prot: sp|Q6NS26|CDKAL_XENLA Threonylcarbamoyladenosine tRNA methylthiotransferase; TrEMBL-Plants: tr|I1LNN6|I1LNN6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0556600 | 2 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Molecular function | 4 iron, 4 sulfur cluster binding | 1 | 12.50 |