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| Field | Value |
|---|---|
| Gene ID | LotjaGi2g1v0385400 |
| Transcript ID | LotjaGi2g1v0385400.1 |
| Related isoforms 3 | |
| Lotus japonicus genome version | Gifu v1.2 |
| Description | tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT4G36390.1 Methylthiotransferase; Swiss-Prot: sp|Q8H0V1|CK5P1_ARATH CDK5RAP1-like protein; TrEMBL-Plants: tr|G7KGB3|G7KGB3_MEDTR Radical SAM methylthiotransferase, MiaB/RimO family protein; Found in the gene: LotjaGi2g1v0385400 |
| Working Lj name | n.a. |
Genomic sequence (LjG1.1_chr2:88149599..88153156) extracted from Lotus japonicus Gifu genome v1.2.
CGACGAGAACCTTTTGTGTTGGGGCGTCTTGTTGTTGTGACCGCTCAGACCGGACCCTATCCACCTCCACCACCACTATC ACTATGTGAGCGTTTCTGTTTGCGTCTCAGTTCAATTCCGATAATGGCATCGTCACTGTCCTCCATCATCAACCAACCTC ACACTGCTCTCAAGCTTCACCCTCCATCTTGTTTCACCACCACTCTCAGAATCTTCTACCACCAACACAAATTCAAACAC AGCACTTCTTCTCTTCCACGCACATATGGCTATCGCGCCTCCACTTCACCGCCGCGCCCTCTCAATCTCATCACCGCCTC CACAACCTTCCCTCGTTCCACCAAATTCGAGGGCCCCACTCTCCGCCGCTGCTTCATCGCTCAAGCCACTCTCGCCGCTA CCCAACAACCCCAACAAGAGTGAGTCATTGCAAACACACCCCACCTCTTTGTTTTGTTCTGGGTTTTCATTACTGTATGA TTCTTCAATGGAACTGCCTTCAAAATTTAATTTTTTTCTTCATTTGCATTCTGGGTATTTTTGCAAACACCCCCACTTTG TGGGAGAATAATACAAGAATATTCATAGGACAGATTAGAGAATGTTGAAAAACAGTGGATGAAATGAAACCAATCATTTA TATTCTTTCAGCAATTTATATTCCCTTATGAATTTGTTGAACTACTGAGTTTAGGTTGGATTGATTAGGAGTGAAATGTC TTTACTGGTTATTTATTTGTTTATTTTTTATTTGTGAGGTAGCCTTGTACTGGATACTGAAGTTCCGTCAACCGGTCGTG TATATCATGAGACTTATGGATGTCAAATGAATGTTAATGACATGGAGATTGTCCTGTCTATAATGAAAAACGCTGGCTAT AATGAAGTTGTGAGTGCTCCTGAGAGTGCAGAAATAATATTTATCAACACTTGTGCCATCAGAGAGAATGCGGAACTTAA AGTATGGCAGAGGCTTAATTACTTTTGGTTTCTTAAGAGGAATTGGAAGGCCAATGTTGCTTCGGGAAGGGCTCAGTCCC TGCACCCTCCGAAAGTAGTTGTCTTGGGCTGTATGGCTGAGAGGCTAAAGGAGAAGATACTTGATGCCGACAAAATGGTT GACGTCGTCTGCGGACCTGATGCATATAGGGATTTGCCGCGATTACTGGAAGAGGTTGACGACGGCCAGAAAGGAATCAA CACTCTTCTTTCACTTGAGGAGACTTATGCTGATATTAATCCAGTTCGAATCTCCAAGAACTCGGTTTCCGCTTTTGTCT CCGTGATGAGGGGTTGCAACAATATGTGCTCATTTTGTATTGTACCTTTCACTAGAGGCAGAGAGCGGTCACGTCCTGTG GAATCCATTGTGAGAGAGGTGGCAGAGCTGTGGAAAGAGGGTGTGAAAGAAGTAACACTTCTTGGCCAGAACGTGAACAG CTATAATGATACATCTGGTTTTGAAAAAGAGGTTGAATCAGGATCCAATTGGAAACTTAGTGATGGATTTTCCAGCATAT CCAAGGTCAAGAAAATGGGCTTAAGGTTTTCTGATCTATTGGATCGACTCTCCTCAGAGTTTCCTGAGATGCGATTCAGA TTCACCTCCCCACACCCTAAAGATTTTCCAGACGAATTGCTCTATCTTATGCGAGAAAGACATAATATATGCAAAGTTAT ACATTTACCTGCACAAACAGGTAGTAGCACTGTGCTTGAGAGAATGAGACGAGGATACACTCGAGAGGCTTACTTGGATC TTGTGCACAAAATTCGAAGTGTTATTCCAGATGTGGGCTTAAGCAGTGATTTTATATGTGGTAATATGTTTTTCACGTTT CTTTCTCCATGTCTCTTTACAGCCCTCTATTTCATAATCATGTTTTGTCCGGTGATCTTTTATTCTTTGGGAACTCGGTG TTCTAATTTCCCTAAGCTATTTAGGTTCAGGTTAAACAAATTTCTACATTGTAATTACCATCTGACATACATCTTTAGAG GTGTTGCACTTCTGAAATTATTGAGAACTTTTTCAAATTTCAATAACTCTTCCAAACAAAATAGTAGAGGTTCCTTTTTT GTGTTAAGAGTTCTGTGGAAGGGATATATGAAATTTCTGTTCGCAAATATGATCATCTAGTTATTTGATTTGTTATTGAG GAGTAGTACCGTATCAAGCAACATGTTTATTCTGTTGGAAGTACTGAAGCAATATTAGTTCATTGATAATTTAGCTATAG TAGATATAGTATTAGTACCCAAAGCTTTCTACCTTGTACTTCTTTGCCACACTAATATTTAGCCTCTGATTTCTGTCTCT TAATATTTGCAATTGTAACAGGTTTTTGTGGAGAAACAGAGGAGGACCACTCTGACACGTTAACTCTCGTGAAAGCTGTT GGTTATGATATGGCATACATGTTTGCTTATAGCATGAGGGAGAAAACACACGCTCACAGAAATTATGCTGATGATGTTCC CGAGGAAGTAAAGCAAAGGAGGCTAACTGAACTCATCGAGACTTTCCGCGAGAGCACAGGAAAGTGCTATGACTCTCAGG TTGGAACTACACAGCTTGTGTTAGTTGAAGGACCCAATAAGAGAGCTCCGGACACCGAGCTTATGGGCAAAAGTGACAAG GGCCATCGGGTGCTATTTGCCAATGTGCCGATCCCAGACAGGGACGATGTTAATACAATGAGAAATCCTGTTGTTGGTGA TTTCGTTGAAGTCCGCATAATGAGATCTACTAGGGCATCACTCTTCGGTGAATCACTTGCTATAACTAAACTGAGTTCAT TCTACAATGATCTAGATAAAGAAGCTGTTGCATGCAACATATAAAACCTTGCAGCATAAACAGAAACAGCTTGGCATGCC AATTTTTGGACGAAGTCATGTAGATTGGTTTTTCCCTTGCTTCGTTTACTGCACATCACATTTCATTTCATGCAAGGGAG TGAGATTGTGCATTTACCCGCTACATGTCAGCTAGTTCAAAATTGCTTCCTCTGTTAATCTTCTCTTAACATGAAATGGA CCCAAGTCTGCATTAAATTCTACACTGAGAATCTGATTGGCAACCTGCAATACAACACAGAAACAAATAGAGCAAGGGAT TGCTGAGATTTTTGTATTTTAACATTGGTTTGATAAGTGAGCTTTTGATGAAGCACACGGAAGAATGTAGCCTTTGGCCC ATCATATTTCTTGTTTCAACTGAACATCACAGGTCTAACGAAGTCTATTGTCACCATTCATCAAATTTCGTTGTAGATGA AACTCATCTAAAATAAGCATGGCTCTAGATGAACATATCTTTAGTTTCACCTTAGATTTTCATTTCACACGGAAGCTTAT TCTAGATGAGCTTCTTTCTTTGGTATCACAGCATGTCAGAATTAGTTTATCATTTCGTAGAATCAATTTTGACATCTCGA TCCTACTAAACAACTTTTTCTCAAAAAAAATGTCTTCAAAATCATTTGTAGCATGTTTGGGGCACTTAAATCAACTGTAG CATGATTTCCAAACCTGCACTTTGAAACAACTTTTTTT
CDS sequence (LotjaGi2g1v0385400.1) extracted from Lotus japonicus Gifu v1.2 CDS.
ATGGCATCGTCACTGTCCTCCATCATCAACCAACCTCACACTGCTCTCAAGCTTCACCCTCCATCTTGTTTCACCACCAC TCTCAGAATCTTCTACCACCAACACAAATTCAAACACAGCACTTCTTCTCTTCCACGCACATATGGCTATCGCGCCTCCA CTTCACCGCCGCGCCCTCTCAATCTCATCACCGCCTCCACAACCTTCCCTCGTTCCACCAAATTCGAGGGCCCCACTCTC CGCCGCTGCTTCATCGCTCAAGCCACTCTCGCCGCTACCCAACAACCCCAACAAGACCTTGTACTGGATACTGAAGTTCC GTCAACCGGTCGTGTATATCATGAGACTTATGGATGTCAAATGAATGTTAATGACATGGAGATTGTCCTGTCTATAATGA AAAACGCTGGCTATAATGAAGTTGTGAGTGCTCCTGAGAGTGCAGAAATAATATTTATCAACACTTGTGCCATCAGAGAG AATGCGGAACTTAAAGTATGGCAGAGGCTTAATTACTTTTGGTTTCTTAAGAGGAATTGGAAGGCCAATGTTGCTTCGGG AAGGGCTCAGTCCCTGCACCCTCCGAAAGTAGTTGTCTTGGGCTGTATGGCTGAGAGGCTAAAGGAGAAGATACTTGATG CCGACAAAATGGTTGACGTCGTCTGCGGACCTGATGCATATAGGGATTTGCCGCGATTACTGGAAGAGGTTGACGACGGC CAGAAAGGAATCAACACTCTTCTTTCACTTGAGGAGACTTATGCTGATATTAATCCAGTTCGAATCTCCAAGAACTCGGT TTCCGCTTTTGTCTCCGTGATGAGGGGTTGCAACAATATGTGCTCATTTTGTATTGTACCTTTCACTAGAGGCAGAGAGC GGTCACGTCCTGTGGAATCCATTGTGAGAGAGGTGGCAGAGCTGTGGAAAGAGGGTGTGAAAGAAGTAACACTTCTTGGC CAGAACGTGAACAGCTATAATGATACATCTGGTTTTGAAAAAGAGGTTGAATCAGGATCCAATTGGAAACTTAGTGATGG ATTTTCCAGCATATCCAAGGTCAAGAAAATGGGCTTAAGGTTTTCTGATCTATTGGATCGACTCTCCTCAGAGTTTCCTG AGATGCGATTCAGATTCACCTCCCCACACCCTAAAGATTTTCCAGACGAATTGCTCTATCTTATGCGAGAAAGACATAAT ATATGCAAAGTTATACATTTACCTGCACAAACAGGTAGTAGCACTGTGCTTGAGAGAATGAGACGAGGATACACTCGAGA GGCTTACTTGGATCTTGTGCACAAAATTCGAAGTGTTATTCCAGATGTGGGCTTAAGCAGTGATTTTATATGTGGTTTTT GTGGAGAAACAGAGGAGGACCACTCTGACACGTTAACTCTCGTGAAAGCTGTTGGTTATGATATGGCATACATGTTTGCT TATAGCATGAGGGAGAAAACACACGCTCACAGAAATTATGCTGATGATGTTCCCGAGGAAGTAAAGCAAAGGAGGCTAAC TGAACTCATCGAGACTTTCCGCGAGAGCACAGGAAAGTGCTATGACTCTCAGGTTGGAACTACACAGCTTGTGTTAGTTG AAGGACCCAATAAGAGAGCTCCGGACACCGAGCTTATGGGCAAAAGTGACAAGGGCCATCGGGTGCTATTTGCCAATGTG CCGATCCCAGACAGGGACGATGTTAATACAATGAGAAATCCTGTTGTTGGTGATTTCGTTGAAGTCCGCATAATGAGATC TACTAGGGCATCACTCTTCGGTGAATCACTTGCTATAACTAAACTGAGTTCATTCTACAATGATCTAGATAAAGAAGCTG TTGCATGCAACATATAA
Protein sequence (LotjaGi2g1v0385400.1) extracted from Lotus japonicus Gifu v1.2 Proteins.
MASSLSSIINQPHTALKLHPPSCFTTTLRIFYHQHKFKHSTSSLPRTYGYRASTSPPRPLNLITASTTFPRSTKFEGPTL RRCFIAQATLAATQQPQQDLVLDTEVPSTGRVYHETYGCQMNVNDMEIVLSIMKNAGYNEVVSAPESAEIIFINTCAIRE NAELKVWQRLNYFWFLKRNWKANVASGRAQSLHPPKVVVLGCMAERLKEKILDADKMVDVVCGPDAYRDLPRLLEEVDDG QKGINTLLSLEETYADINPVRISKNSVSAFVSVMRGCNNMCSFCIVPFTRGRERSRPVESIVREVAELWKEGVKEVTLLG QNVNSYNDTSGFEKEVESGSNWKLSDGFSSISKVKKMGLRFSDLLDRLSSEFPEMRFRFTSPHPKDFPDELLYLMRERHN ICKVIHLPAQTGSSTVLERMRRGYTREAYLDLVHKIRSVIPDVGLSSDFICGFCGETEEDHSDTLTLVKAVGYDMAYMFA YSMREKTHAHRNYADDVPEEVKQRRLTELIETFRESTGKCYDSQVGTTQLVLVEGPNKRAPDTELMGKSDKGHRVLFANV PIPDRDDVNTMRNPVVGDFVEVRIMRSTRASLFGESLAITKLSSFYNDLDKEAVACNI
Data for domain prediction are obtained with InterProScan, and merged with InterPro data obtained from the EB-eye REST service.
| Prediction algorithm | Identifier | Start | End | Length | E-value | InterPro ID |
|---|---|---|---|---|---|---|
| PANTHER | 94 | 606 | 513 | 1.2E-264 | – | |
| PANTHER | 94 | 606 | 513 | 1.2E-264 | – | |
| SFLD | 109 | 602 | 494 | 0.0 | ||
| SFLD | 109 | 602 | 494 | 0.0 | – | |
| SFLD | 109 | 598 | 490 | 0.0 | ||
| ProSiteProfiles | 110 | 239 | 130 | 37.039 | ||
| Gene3D | 111 | 255 | 145 | 3.4E-26 | ||
| Pfam | 111 | 223 | 113 | 3.2E-30 | ||
| TIGRFAM | 111 | 593 | 483 | 6.0E-115 | ||
| TIGRFAM | 112 | 595 | 484 | 5.7E-116 | – | |
| SFLD | 171 | 492 | 322 | 8.2E-29 | – | |
| SUPERFAMILY | 261 | 515 | 255 | 5.23E-55 | – | |
| Gene3D | 265 | 523 | 259 | 4.3E-84 | ||
| SMART | 267 | 508 | 242 | 4.0E-52 | ||
| Pfam | 271 | 467 | 197 | 3.8E-35 | ||
| ProSitePatterns | 271 | 291 | 21 | - | ||
| CDD | 275 | 493 | 219 | 9.1866E-8 | – | |
| Pfam | 522 | 595 | 74 | 2.8E-13 | ||
| ProSiteProfiles | 522 | 598 | 77 | 14.127 |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Catalytic activity | Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic. | ||
| Biological process | TRNA modification | The covalent alteration of one or more nucleotides within a tRNA molecule to produce a tRNA molecule with a sequence that differs from that coded genetically. | ||
| Molecular function | Transferase activity | Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2. | ||
| Molecular function | Methylthiotransferase activity | Catalysis of the addition of a methylthioether group (-SCH3) to a nucleic acid or protein acceptor. | ||
| Molecular function | Iron-sulfur cluster binding | Interacting selectively and non-covalently with an iron-sulfur cluster, a combination of iron and sulfur atoms. | ||
| Molecular function | 4 iron, 4 sulfur cluster binding | Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. |
Expression pattern of LotjaGi2g1v0385400.1, powered by ExpAt. For advanced configuration, data transformation and export options, view expression data in the ExpAt application.
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