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IPR003154 is a S1/P1 nuclease.
<p>This family summarizes both S1 and P1 nucleases ([ec:3.1.30.1]) which cleave RNA and single stranded DNA with no base specificity [[cite:PUB00080593]]. S1 nuclease is more active on DNA than RNA. Its reaction products are oligonucleotides or single nucleotides with 5' phosphoryl groups [[cite:PUB00080594]]. Although its primary substrate is single-stranded, it may also introduce single-stranded breaks in double-stranded DNA or RNA, or DNA-RNA hybrids. It is used as a reagent in nuclease protection assays and in removing single stranded tails from DNA molecules to create blunt ended molecules and opening hairpin loops generated during synthesis of double stranded cDNA. P1 nuclease cleaves its substrate at every position yielding nucleoside 5' monophosphates, and it does not recognize or act on double-stranded DNA [[cite:PUB00020287]]. It is useful at removing single stranded strands hanging off the end of double stranded DNA and at completely cleaving melted DNA for simple DNA composition analysis.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Nucleic acid binding | Interacting selectively and non-covalently with any nucleic acid. | ||
| Molecular function | Endonuclease activity | Catalysis of the hydrolysis of ester linkages within nucleic acids by creating internal breaks. | ||
| Biological process | DNA catabolic process | The cellular DNA metabolic process resulting in the breakdown of DNA, deoxyribonucleic acid, one of the two main types of nucleic acid, consisting of a long unbranched macromolecule formed from one or two strands of linked deoxyribonucleotides, the 3'-phosphate group of each constituent deoxyribonucleotide being joined in 3',5'-phosphodiester linkage to the 5'-hydroxyl group of the deoxyribose moiety of the next one. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Nuclease S1 [Medicago truncatula] gi|357488641|ref|XP_003614608.1| | 14 | ||
| – | Endonuclease [Medicago truncatula] gi|357488645|ref|XP_003614610.1| | 7 | ||
| – | Nuclease S1 [Medicago truncatula] gi|357488641|ref|XP_003614608.1| | 8 | ||
| – | PREDICTED: endonuclease 4-like isoform X1 [Cicer arietinum] gi|502142497|ref|XP_004504986.1| | 15 | ||
| – | PREDICTED: nuclease S1-like [Glycine max] gi|356575560|ref|XP_003555908.1| | 14 | ||
| – | Endonuclease [Medicago truncatula] gi|357446373|ref|XP_003593464.1| | 14 | ||
| – | Endonuclease, partial [Lotus japonicus] gi|50657594|gb|AAT79581.1| | 8 | ||
| – | Nuclease S1; TAIR: AT1G68290.1 endonuclease 2; Swiss-Prot: sp|Q9C9G4|ENDO2_ARATH Endonuclease 2; TrEMBL-Plants: tr|I1J6M1|I1J6M1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0276000 | 15 | ||
| – | Nuclease S1; TAIR: AT1G68290.1 endonuclease 2; Swiss-Prot: sp|Q9C9G4|ENDO2_ARATH Endonuclease 2; TrEMBL-Plants: tr|A0A1J7HPH3|A0A1J7HPH3_LUPAN Uncharacterized protein; Found in the gene: LotjaGi2g1v0276000 | 8 | ||
| – | Nuclease S1; TAIR: AT1G68290.1 endonuclease 2; Swiss-Prot: sp|Q9C9G4|ENDO2_ARATH Endonuclease 2; TrEMBL-Plants: tr|A0A1J7HPH3|A0A1J7HPH3_LUPAN Uncharacterized protein; Found in the gene: LotjaGi2g1v0276000 | 12 | ||
| – | Nuclease S1; TAIR: AT4G21585.1 endonuclease 4; Swiss-Prot: sp|F4JJL0|ENDO4_ARATH Endonuclease 4; TrEMBL-Plants: tr|I3SW41|I3SW41_LOTJA Uncharacterized protein; Found in the gene: LotjaGi4g1v0050700 | 14 | ||
| – | Nuclease S1; TAIR: AT1G68290.1 endonuclease 2; Swiss-Prot: sp|Q9C9G4|ENDO2_ARATH Endonuclease 2; TrEMBL-Plants: tr|I1NFK4|I1NFK4_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0339100 | 14 | ||
| – | Nuclease S1; TAIR: AT1G11190.1 bifunctional nuclease i; Swiss-Prot: sp|Q9SXA6|ENDO1_ARATH Endonuclease 1; TrEMBL-Plants: tr|I3SHH3|I3SHH3_LOTJA Uncharacterized protein; Found in the gene: LotjaGi6g1v0337400 | 14 | ||
| – | Nuclease S1; TAIR: AT1G11190.1 bifunctional nuclease i; Swiss-Prot: sp|Q9SXA6|ENDO1_ARATH Endonuclease 1; TrEMBL-Plants: tr|I3SHH3|I3SHH3_LOTJA Uncharacterized protein; Found in the gene: LotjaGi6g1v0337400 | 8 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd11010 | CDD | 1 | 7.14 |