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IPR006048 is a Alpha-amylase/branching enzyme, C-terminal all beta.
<p>This entry represents the all-β C-terminal domain that is found in members of the glycosyl hydrolase 13 family, such as alpha-amylases and 1,4-alpha-glucan branching enzyme. This domain forms a Greek key β-barrel fold in these enzymes [[cite:PUB00023681]].</p> <p>Alpha-amylase is classified as family 13 ([cazy:GH13]) of the glycosyl hydrolases and is present in archaea, bacteria, fungi, plants and animals. Alpha-amylase is an essential enzyme in alpha-glucan metabolism, acting to catalyse the hydrolysis of alpha-1,4-glucosidic bonds of glycogen, starch and related polysaccharides. Although all alpha-amylases possess the same catalytic function, they can vary with respect to sequence. In general, they are composed of three domains: a TIM barrel containing the active site residues and chloride ion-binding site (domain A), a long loop region inserted between the third β-strand and the α-helix of domain A that contains calcium-binding site(s) (domain B), and a C-terminal β-sheet domain that appears to show some variability in sequence and length between amylases (domain C) [[cite:PUB00027666]]. Amylases have at least one conserved calcium-binding site, as calcium is essential for the stability of the enzyme. The chloride-binding functions to activate the enzyme, which acts by a two-step mechanism involving a catalytic nucleophile base (usually an Asp) and a catalytic proton donor (usually a Glu) that are responsible for the formation of the beta-linked glycosyl-enzyme intermediate.</p> <p>Branching enzyme catalyses the formation of alpha-1,6 branch points in either glycogen or starch. It has an important role in the determination of the structure of starch in plants and of glycogen in animals and bacteria [[cite:PUB00027252]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Catalytic activity | Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic. | ||
| Biological process | Carbohydrate metabolic process | The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule. | ||
| Molecular function | Cation binding | Interacting selectively and non-covalently with cations, charged atoms or groups of atoms with a net positive charge. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: 1,4-alpha-glucan-branching enzyme-like isoform X1 [Cicer arietinum] gi|502131109|ref|XP_004500878.1| | 25 | ||
| – | PREDICTED: 1,4-alpha-glucan-branching enzyme-like [Glycine max] gi|356569752|ref|XP_003553060.1| | 12 | ||
| – | PREDICTED: 1,4-alpha-glucan-branching enzyme-like [Glycine max] gi|356508675|ref|XP_003523080.1| | 25 | ||
| – | Starch branching enzyme I [Pisum sativum] gi|1345570|emb|CAA56319.1| | 25 | ||
| – | 1,4-alpha-glucan branching enzyme GlgB; TAIR: AT4G09020.1 isoamylase 3; Swiss-Prot: sp|Q9M0S5|ISOA3_ARATH Isoamylase 3, chloroplastic; TrEMBL-Plants: tr|K7KU93|K7KU93_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0340200 | 21 | ||
| – | 1,4-alpha-glucan-branching enzyme; TAIR: AT5G03650.1 starch branching enzyme 2.2; Swiss-Prot: sp|Q41059|GLGB2_PEA 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic; TrEMBL-Plants: tr|K7KHN1|K7KHN1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0436100 | 23 | ||
| – | 1,4-alpha-glucan-branching enzyme; TAIR: AT5G03650.1 starch branching enzyme 2.2; Swiss-Prot: sp|Q41059|GLGB2_PEA 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic; TrEMBL-Plants: tr|X5CRZ1|X5CRZ1_LENCU Starch branching protein I; Found in the gene: LotjaGi1g1v0436100 | 23 | ||
| – | 1,4-alpha-glucan-branching enzyme; TAIR: AT5G03650.1 starch branching enzyme 2.2; Swiss-Prot: sp|Q41058|GLGB1_PEA 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic; TrEMBL-Plants: tr|A0A072U332|A0A072U332_MEDTR Starch branching enzyme I; Found in the gene: LotjaGi1g1v0678900 | 23 | ||
| – | 1,4-alpha-glucan-branching enzyme; TAIR: AT5G03650.1 starch branching enzyme 2.2; Swiss-Prot: sp|Q41058|GLGB1_PEA 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic; TrEMBL-Plants: tr|A0A072U332|A0A072U332_MEDTR Starch branching enzyme I; Found in the gene: LotjaGi1g1v0678900 | 23 | ||
| – | 1,4-alpha-glucan-branching enzyme; TAIR: AT5G03650.1 starch branching enzyme 2.2; Swiss-Prot: sp|Q41058|GLGB1_PEA 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic; TrEMBL-Plants: tr|X5CLX6|X5CLX6_LENCU Starch branching protein II; Found in the gene: LotjaGi1g1v0678900 | 23 | ||
| – | 1,4-alpha-glucan branching enzyme, putative; TAIR: AT3G20440.2 Alpha amylase family protein; Swiss-Prot: sp|D2WL32|GLGB3_ARATH 1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic; TrEMBL-Plants: tr|K7MQT1|K7MQT1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0156000 | 23 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 9.09 |