Your browser is unable to support new features implemented in HTML5 and CSS3 to render this site as intended. Your experience may suffer from functionality degradation but the site should remain usable. We strongly recommend the latest version of Google Chrome, OS X Safari or Mozilla Firefox. As Safari is bundled with OS X, if you are unable to upgrade to a newer version of OS X, we recommend using an open source browser. Dismiss message
IPR008915 is a Peptidase M50.
<p>Over 70 metallopeptidase families have been identified to date. In these enzymes a divalent cation which is usually zinc, but may be cobalt, manganese or copper, activates the water molecule. The metal ion is held in place by amino acid ligands, usually three in number. In some families of co-catalytic metallopeptidases, two metal ions are observed in crystal structures ligated by five amino acids, with one amino acid ligating both metal ions. The known metal ligands are His, Glu, Asp or Lys. At least one other residue is required for catalysis, which may play an electrophillic role. Many metalloproteases contain an HEXXH motif, which has been shown in crystallographic studies to form part of the metal-binding site [[cite:PUB00003579]]. The HEXXH motif is relatively common, but can be more stringently defined for metalloproteases as 'abXHEbbHbc', where 'a' is most often valine or threonine and forms part of the S1' subsite in thermolysin and neprilysin, 'b' is an uncharged residue, and 'c' a hydrophobic residue. Proline is never found in this site, possibly because it would break the helical structure adopted by this motif in metalloproteases [[cite:PUB00003579]].</p> <p>This entry contains metallopeptidases belonging to MEROPS peptidase family M50 (S2P protease family, clan MM).</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Metalloendopeptidase activity | Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions. | ||
| Biological process | Proteolysis | The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: membrane-bound transcription factor site-2 protease-like [Glycine max] gi|356560965|ref|XP_003548756.1| | 13 | ||
| – | Serine protease [Arabidopsis thaliana] gi|18402981|ref|NP_565745.1| | 15 | ||
| – | M50 family peptidase; TAIR: AT5G35220.1 Peptidase M50 family protein; Swiss-Prot: sp|Q949Y5|EGY1_ARATH Probable zinc metalloprotease EGY1, chloroplastic; TrEMBL-Plants: tr|A0A0L9U7J7|A0A0L9U7J7_PHAAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0647400 | 10 | ||
| – | Membrane-associated zinc metalloprotease family protein, expressed; TAIR: AT2G32480.1 chloroplastic membrane metallo proteases; Swiss-Prot: sp|O80885|ARASP_ARATH Membrane metalloprotease ARASP, chloroplastic; TrEMBL-Plants: tr|V7AFZ2|V7AFZ2_PHAVU Uncharacterized protein; Found in the gene: LotjaGi3g1v0467200 | 17 | ||
| – | Protease m50 membrane-bound transcription factor site 2 protease, putative; TAIR: AT4G20310.4 Peptidase M50 family protein; Swiss-Prot: sp|F4JUU5|S2P_ARATH Membrane-bound transcription factor site-2 protease homolog; TrEMBL-Plants: tr|A0A0B2Q9W2|A0A0B2Q9W2_GLYSO Membrane-bound transcription factor site-2 protease; Found in the gene: LotjaGi4g1v0149600 | 11 | ||
| – | Protease m50 membrane-bound transcription factor site 2 protease, putative; TAIR: AT4G20310.4 Peptidase M50 family protein; Swiss-Prot: sp|F4JUU5|S2P_ARATH Membrane-bound transcription factor site-2 protease homolog; TrEMBL-Plants: tr|A0A0B2Q9W2|A0A0B2Q9W2_GLYSO Membrane-bound transcription factor site-2 protease; Found in the gene: LotjaGi4g1v0149600 | 12 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| TRANSMEMBRANE | Phobius | 1 | 16.67 |