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IPR035107 is a tRNA thiolation protein, TtcA/Ctu1 type.
<p>This entry represents a group of tRNA thiolation proteins. It includes the tRNA-cytidine(32) 2-sulfurtransferase ([interpro:IPR012089]), and Cytoplasmic tRNA 2-thiolation protein 1 ([interpro:IPR000541]).</p> <p>tRNA-cytidine(32) 2-sulfurtransferase (also known as 2-thiocytidine tRNA biosynthesis protein TtcA) is required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32). The modified nucleoside 2-thiocytidine (s(2)C) has so far been found in tRNA from archaea and bacteria. The TtcA protein family is characterised by the existence of both a PP-loop and a Cys-X(1)-X(2)-Cys motif in the central region of the protein but can be divided into two distinct groups based on the presence and location of additional Cys-X(1)-X(2)-Cys motifs in terminal regions of the sequence. Mutant analysis showed that both cysteines in this central conserved Cys-X(1)-X(2)-Cys motif are required for the formation of s(2)C [[cite:PUB00044701]].</p> <p>Cytoplasmic tRNA 2-thiolation protein 1 (also known as Ncs6/Tuc1 in budding yeasts) is responsible for 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln) [[cite:PUB00069585], [cite:PUB00045305]]. It directly binds tRNAs and probably acts by catalysing adenylation of tRNAs, an intermediate required for 2-thiolation. Its fission yeast homologue, Ctu1 forms a complex with Ctu2 (Ncs2/Tuc2 homologue) and serves as a putative enzyme for the formation of 2-thiouridine [[cite:PUB00069587]]. This family also includes tRNA-5-methyluridine(54) 2-sulfurtransferase, which catalyses the 2-thiolation of 5-methyluridine residue at position 54 in the T loop of tRNAs, leading to 5-methyl-2-thiouridine [[cite:PUB00087328]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | TRNA processing | The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Cytoplasmic tRNA 2-thiolation protein; TAIR: AT2G44270.1 repressor of lrx1; Swiss-Prot: sp|O64862|CTU1_ARATH Cytoplasmic tRNA 2-thiolation protein 1; TrEMBL-Plants: tr|G7J985|G7J985_MEDTR Cytoplasmic tRNA 2-thiolation protein 1; Found in the gene: LotjaGi1g1v0556900 | 16 | ||
| – | Cytoplasmic tRNA 2-thiolation protein; TAIR: AT2G44270.1 repressor of lrx1; Swiss-Prot: sp|O64862|CTU1_ARATH Cytoplasmic tRNA 2-thiolation protein 1; TrEMBL-Plants: tr|V7AXH9|V7AXH9_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0556900 | 14 | ||
| – | Cytoplasmic tRNA 2-thiolation protein; TAIR: AT2G44270.1 repressor of lrx1; Swiss-Prot: sp|O64862|CTU1_ARATH Cytoplasmic tRNA 2-thiolation protein 1; TrEMBL-Plants: tr|G7J985|G7J985_MEDTR Cytoplasmic tRNA 2-thiolation protein 1; Found in the gene: LotjaGi1g1v0556900 | 15 | ||
| – | Cytoplasmic tRNA 2-thiolation protein; TAIR: AT2G44270.1 repressor of lrx1; Swiss-Prot: sp|O64862|CTU1_ARATH Cytoplasmic tRNA 2-thiolation protein 1; TrEMBL-Plants: tr|G7J985|G7J985_MEDTR Cytoplasmic tRNA 2-thiolation protein 1; Found in the gene: LotjaGi1g1v0556900 | 15 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 25.00 |