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Field | Value |
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Namespace | Biological process |
Short description | Ubiquitin-dependent ERAD pathway |
Full defintion | The series of steps necessary to target endoplasmic reticulum (ER)-resident proteins for degradation by the cytoplasmic proteasome. Begins with recognition of the ER-resident protein, includes retrotranslocation (dislocation) of the protein from the ER to the cytosol, protein ubiquitination necessary for correct substrate transfer, transport of the protein to the proteasome, and ends with degradation of the protein by the cytoplasmic proteasome. |
Subterm of |
The relationship of GO:0030433 with other GO terms.
Relationship type | GO terms |
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Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0030433, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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GOC | rb |
PMID | Degradation of sterol regulatory element-binding protein precursor requires the endoplasmic reticulum-associated degradation components Ubc7 and Hrd1 in fission yeast. J Biol Chem. 2009 Jul 31; 284 (31): 20512–21.PMID: 19520858 Sre1, the fission yeast sterol regulatory element-binding protein (SREBP), is an endoplasmic reticulum (ER) membrane-bound transcription factor that is a principal regulator of hypoxic gene expression. Under low oxygen, Sre1 is cleaved from its inactive ER precursor form to generate an active nuclear transcription factor that up-regulates genes required for low oxygen growth. To maintain a constant supply of Sre1, Sre1 precursor synthesis must be regulated to replenish Sre1 precursor lost to proteolytic cleavage under low oxygen. In this study, we investigated the mechanisms controlling Sre1 precursor levels. We found that positive feedback regulation at the sre1(+) promoter increases the synthesis of the Sre1 precursor under low oxygen and that this regulation is required for maximal Sre1 activation and target gene expression. We also demonstrate that the Sre1 precursor is rapidly degraded by the proteasome in the absence of its binding partner Scp1, which is required for oxygen-regulated Sre1 cleavage. Degradation of Sre1 in the absence of Scp1 requires the ER-associated degradation (ERAD) components Ubc7, an E2 ubiquitin conjugating enzyme, and Hrd1, an E3 ubiquitin ligase. We conclude that positive feedback regulation to up-regulate Sre1 precursor synthesis under low oxygen is essential for Sre1 function and propose that excess Sre1 precursor is removed by ERAD to ensure complex formation between Sre1 and its binding partner Scp1. Thus, Sre1 is a new example of an endogenous ERAD substrate, establishing fission yeast as an organism for the study of this important degradative pathway. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: E3 ubiquitin-protein ligase synoviolin-like [Glycine max] gi|356538686|ref|XP_003537832.1| | 4 | ||
– | 4 | |||
– | E3 ubiquitin-protein ligase synoviolin [Medicago truncatula] gi|357481129|ref|XP_003610850.1| | 4 | ||
– | Ubiquitin thioesterase OTU1; TAIR: AT1G50670.1 OTU-like cysteine protease family protein; Swiss-Prot: sp|Q567B1|OTU1_DANRE Ubiquitin thioesterase OTU1; TrEMBL-Plants: tr|I3T4E6|I3T4E6_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0029400 | 4 | ||
– | Ubiquitin thioesterase OTU1; TAIR: AT1G50670.1 OTU-like cysteine protease family protein; Swiss-Prot: sp|Q567B1|OTU1_DANRE Ubiquitin thioesterase OTU1; TrEMBL-Plants: tr|I3T4E6|I3T4E6_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0029400 | 4 | ||
– | Ubiquitin thioesterase OTU1; TAIR: AT1G50670.1 OTU-like cysteine protease family protein; Swiss-Prot: sp|Q55BI3|OTU1_DICDI Ubiquitin thioesterase OTU1; TrEMBL-Plants: tr|I3T4E6|I3T4E6_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0029400 | 4 | ||
– | OTU domain-containing protein; TAIR: AT2G38025.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|Q8LBZ4|OTU_ARATH OTU domain-containing protein At3g57810; TrEMBL-Plants: tr|I3TAS0|I3TAS0_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0215900 | 4 | ||
– | Otu domain-containing protein; TAIR: AT2G38025.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|Q8LBZ4|OTU_ARATH OTU domain-containing protein At3g57810; TrEMBL-Plants: tr|I3TAS0|I3TAS0_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0215900 | 4 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Molecular function | Ubiquitinyl hydrolase activity | 1 | 12.50 |