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IPR001269

Source: InterPro

Description

IPR001269 is a tRNA-dihydrouridine synthase.

<p>Dihydrouridine synthases (Dus) is a large family of flavoenzymes comprising eight subfamilies. They catalyse the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Mainly, they contain two functional conserved domains, an N-terminal catalytic domain (TBD) adopting a TIM barrel fold and a unique C-terminal helical domain (HD) devoted to tRNA recognition. However, DUS2 is distinguished from its paralogues and its fungi orthologues by the acquisition of an additional domain, a double stranded RNA binding domain (dsRBD), which serves as the main tRNA binding module [[cite:PUB00096089], [cite:PUB00096095]].</p> <p>Dus 1 ([swissprot:P53759]) from Saccharomyces cerevisiae (Baker's yeast) acts on pre-tRNA-Phe, while Dus 2 ([swissprot:P53720]) acts on pre-tRNA-Tyr and pre-tRNA-Leu. Dus 1 is active as a single subunit, requiring NADPH or NADH, and is stimulated by the presence of FAD [[cite:PUB00014304]]. Some family members may be targeted to the mitochondria and even have a role in mitochondria [[cite:PUB00014304]]. DUS3 (not included in this entry) contains an extra zinc finger N-terminal to the Dus domain.</p>

This description is obtained from EB-eye REST.

Associated GO terms

GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .

GO term Namespace Name Definition Relationships
GO:0008033
Biological process TRNA processing The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
Subterm of
GO:0017150
Molecular function TRNA dihydrouridine synthase activity Catalysis of the reaction: tRNA-uracil + acceptor = tRNA-dihydrouridine + reduced acceptor.
Subterm of
GO:0050660
Molecular function Flavin adenine dinucleotide binding Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
Subterm of
GO:0055114
Biological process Oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
Subterm of

Associated Lotus transcripts 16

Transcript Name Description Predicted domains Domain count
Lj0g3v0165759.1
– PREDICTED: tRNA-dihydrouridine(20) synthase [NAD](P)+] gi|502154498|ref|XP_004509720.1|
Domains
15
Lj0g3v0208529.1
– PREDICTED: tRNA-dihydrouridine synthase A-like isoform X3 [Cicer arietinum] gi|502127969|ref|XP_004499894.1|
Domains
7
Lj0g3v0208529.2
– PREDICTED: tRNA-dihydrouridine synthase A-like [Glycine max] gi|356537294|ref|XP_003537164.1|
Domains
8
Lj0g3v0208529.3
– PREDICTED: tRNA-dihydrouridine synthase A-like [Glycine max] gi|356537294|ref|XP_003537164.1|
Domains
8
Lj1g3v3354300.1
– PREDICTED: tRNA-dihydrouridine synthase 3-like [Glycine max] gi|356508136|ref|XP_003522816.1|
Domains
15
Lj1g3v3354300.2
– PREDICTED: tRNA-dihydrouridine synthase 3-like [Glycine max] gi|356508136|ref|XP_003522816.1|
Domains
15
Lj3g3v3690100.1
– PREDICTED: tRNA-dihydrouridine synthase A-like isoform X2 [Cicer arietinum] gi|502146150|ref|XP_004506326.1|
Domains
13
Lj4g3v0818960.1
–
Domains
8
Lj4g3v0818970.1
– PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD](P)(+)] gi|502152821|ref|XP_004509110.1|
Domains
11
LotjaGi1g1v0562300.1
– TRNA-dihydrouridine(47) synthase [NAD](P)(+)]; TAIR: AT4G38890.1 FMN-linked oxidoreductases superfamily protein; Swiss-Prot: sp|Q9T0J6|DUS3L_ARATH tRNA-dihydrouridine(47) synthase [NAD](P)(+)]-like; TrEMBL-Plants: tr|K7KHG3|K7KHG3_SOYBN tRNA-dihydrouridine(47) synthase [NAD](P)(+)]; Found in the gene: LotjaGi1g1v0562300
Domains
16
LotjaGi1g1v0562300.2
– TRNA-dihydrouridine(47) synthase [NAD](P)(+)]; TAIR: AT4G38890.1 FMN-linked oxidoreductases superfamily protein; Swiss-Prot: sp|Q9T0J6|DUS3L_ARATH tRNA-dihydrouridine(47) synthase [NAD](P)(+)]-like; TrEMBL-Plants: tr|K7KHG3|K7KHG3_SOYBN tRNA-dihydrouridine(47) synthase [NAD](P)(+)]; Found in the gene: LotjaGi1g1v0562300
Domains
16
LotjaGi3g1v0121800.1
– TRNA-dihydrouridine synthase; TAIR: AT5G47970.1 Aldolase-type TIM barrel family protein; Swiss-Prot: sp|Q9KUX9|DUSA_VIBCH tRNA-dihydrouridine(20/20a) synthase; TrEMBL-Plants: tr|K7LI80|K7LI80_SOYBN tRNA-dihydrouridine synthase; Found in the gene: LotjaGi3g1v0121800
Domains
13
LotjaGi3g1v0179300.1
– Trna-dihydrouridine(47) synthase [nad](P)(+)]-like protein; TAIR: AT3G49640.1 Aldolase-type TIM barrel family protein; Swiss-Prot: sp|Q9NX74|DUS2L_HUMAN tRNA-dihydrouridine(20) synthase [NAD](P)+]-like; TrEMBL-Plants: tr|A0A151SZ46|A0A151SZ46_CAJCA tRNA-dihydrouridine synthase; Found in the gene: LotjaGi3g1v0179300
Domains
15
LotjaGi3g1v0550700.1
– TRNA-dihydrouridine synthase; TAIR: AT3G63510.1 FMN-linked oxidoreductases superfamily protein; Swiss-Prot: sp|Q8Z1T1|DUSA_SALTI tRNA-dihydrouridine(20/20a) synthase; TrEMBL-Plants: tr|I3SNH2|I3SNH2_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0550700
Domains
15
LotjaGi4g1v0101600.1
– TRNA-dihydrouridine synthase-like protein; TAIR: AT5G67220.1 FMN-linked oxidoreductases superfamily protein; Swiss-Prot: sp|Q8C2P3|DUS1L_MOUSE tRNA-dihydrouridine(16/17) synthase [NAD](P)(+)]-like; TrEMBL-Plants: tr|I1K265|I1K265_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0101600
Domains
12
LotjaGi4g1v0101600.2
– TRNA-dihydrouridine synthase-like protein; TAIR: AT5G67220.1 FMN-linked oxidoreductases superfamily protein; Swiss-Prot: sp|Q8C2P3|DUS1L_MOUSE tRNA-dihydrouridine(16/17) synthase [NAD](P)(+)]-like; TrEMBL-Plants: tr|I1K265|I1K265_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0101600
Domains
12

Co-occuring domains 1

A list of co-occurring predicted domains within the L. japonicus gene space:

Predicted domain Source Observations Saturation (%)
mobidb-lite MobiDBLite 1 6.25