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IPR001371 is a Glycoside hydrolase, family 14B, plant.
<p>O-Glycosyl hydrolases ([ec:3.2.1.]) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [[cite:PUB00004870], [cite:PUB00005266]]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.</p> <p>Family 14 ([ec:3.2.1.2], [cazy:GH14]) encompasses the beta-amylase enzymes. Beta-amylases, which are found in plants and bacteria, hydrolyse 1,4-alpha-glucosidic linkages in starch-type polysaccharide substrates, removing successive maltose units from the non-reducing ends of the chains. In Solanum tuberosum (potato), the enzyme has been found to work optimally at 40 degrees C, becoming unstable above this temperature. On the basis of sequence comparisons, plant and bacterial beta-amylases can be readily distinguished from each other.</p> <p>The 3D structure of a complex of soybean beta-amylase with an inhibitor (alpha-cyclodextrin) has been determined to 3.0A resolution by X-ray diffraction [[cite:PUB00002354]]. The enzyme folds into large and small domains: the large domain has a (β α)8 super-secondary structural core, while the smaller is formed from two long loops extending from the β3 and β4 strands of the (β α)8 fold [[cite:PUB00002354]]. The interface of the two domains, together with shorter loops from the (β α)8 core, form a deep cleft, in which the inhibitor binds [[cite:PUB00002354]]. Two maltose molecules also bind in the cleft, one sharing a binding site with alpha-cyclodextrin, and the other sitting more deeply in the cleft [[cite:PUB00002354]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | Polysaccharide catabolic process | The chemical reactions and pathways resulting in the breakdown of a polysaccharide, a polymer of many (typically more than 10) monosaccharide residues linked glycosidically. | ||
| Molecular function | Beta-amylase activity | Catalysis of the reaction: (1,4-alpha-D-glucosyl)(n+1) + H2O = (1,4-alpha-D-glucosyl)(n-1) + alpha-maltose. This reaction is the hydrolysis of 1,4-alpha-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: beta-amylase-like [Cicer arietinum] gi|502083126|ref|XP_004487367.1| | 14 | ||
| – | RecName: Full=Beta-amylase; AltName: Full=1,4-alpha-D-glucan maltohydrolasegi|2559006|gb|AAD04188.1| beta-amylase [Medicago sativa] gi|3913031|sp|O22585.1|AMYB_MEDSA | 19 | ||
| – | Beta-amylase; TAIR: AT3G23920.1 beta-amylase 1; Swiss-Prot: sp|Q9LIR6|BAM1_ARATH Beta-amylase 1, chloroplastic; TrEMBL-Plants: tr|K7MJ07|K7MJ07_SOYBN Beta-amylase; Found in the gene: LotjaGi2g1v0184700 | 14 | ||
| – | Beta-amylase; TAIR: AT2G32290.1 beta-amylase 6; Swiss-Prot: sp|O22585|AMYB_MEDSA Beta-amylase; TrEMBL-Plants: tr|G7IKM6|G7IKM6_MEDTR Beta-amylase; Found in the gene: LotjaGi3g1v0192600 | 13 | ||
| – | Beta-amylase; TAIR: AT4G15210.1 beta-amylase 5; Swiss-Prot: sp|O22585|AMYB_MEDSA Beta-amylase; TrEMBL-Plants: tr|I1LRU3|I1LRU3_SOYBN Beta-amylase; Found in the gene: LotjaGi3g1v0462300 | 18 | ||
| – | Beta-amylase; TAIR: AT4G17090.1 chloroplast beta-amylase; Swiss-Prot: sp|O23553|BAM3_ARATH Beta-amylase 3, chloroplastic; TrEMBL-Plants: tr|I1K0Y6|I1K0Y6_SOYBN Beta-amylase; Found in the gene: LotjaGi4g1v0192900 | 12 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| SSF51445 | SUPERFAMILY | 1 | 16.67 |