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IPR003651 is a Endonuclease III-like, iron-sulphur cluster loop motif.
<p>Endonuclease III ([ec:4.2.99.18]) is a DNA repair enzyme which removes a number of damaged pyrimidines from DNA via its glycosylase activity and also cleaves the phosphodiester backbone at apurinic / apyrimidinic sites via a beta-elimination mechanism [[cite:PUB00007293], [cite:PUB00007294]]. The structurally related DNA glycosylase MutY recognises and excises the mutational intermediate 8-oxoguanine-adenine mispair [[cite:PUB00002202]]. The 3-D structures of Escherichia coli endonuclease III [[cite:PUB00005158]] and catalytic domain of MutY [[cite:PUB00007295]] have been determined. The structures contain two all-alpha domains: a sequence-continuous, six-helix domain (residues 22-132) and a Greek-key, four-helix domain formed by one N-terminal and three C-terminal helices (residues 1-21 and 133-211) together with the Fe4S4 cluster. The cluster is bound entirely within the C-terminal loop by four cysteine residues with a ligation pattern Cys-(Xaa)6-Cys-(Xaa)2-Cys-(Xaa)5-Cys which is distinct from all other known Fe4S4 proteins. This structural motif is referred to as a Fe4S4 cluster loop (FCL) [[cite:PUB00006161]]. Two DNA-binding motifs have been proposed, one at either end of the interdomain groove: the helix-hairpin-helix (HhH) and FCL motifs. The primary role of the iron-sulphur cluster appears to involve positioning conserved basic residues for interaction with the DNA phosphate backbone by forming the loop of the FCL motif [[cite:PUB00006161], [cite:PUB00007296]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | 4 iron, 4 sulfur cluster binding | Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: protein ROS1-like [Glycine max] gi|356503779|ref|XP_003520681.1| | 13 | ||
| – | PREDICTED: protein ROS1-like [Glycine max] gi|356503779|ref|XP_003520681.1| | 14 | ||
| – | PREDICTED: endonuclease III-like protein 1-like [Cicer arietinum] gi|502148937|ref|XP_004507328.1| | 23 | ||
| – | DNA glycosylase; TAIR: AT2G36490.1 demeter-like 1; Swiss-Prot: sp|Q9SJQ6|ROS1_ARATH Protein ROS1; TrEMBL-Plants: tr|A0A0B2QF73|A0A0B2QF73_GLYSO Protein ROS1; Found in the gene: LotjaGi1g1v0679900 | 18 | ||
| – | DNA glycosylase; TAIR: AT2G36490.1 demeter-like 1; Swiss-Prot: sp|Q9SJQ6|ROS1_ARATH Protein ROS1; TrEMBL-Plants: tr|A0A0B2QF73|A0A0B2QF73_GLYSO Protein ROS1; Found in the gene: LotjaGi1g1v0679900 | 18 | ||
| – | DNA glycosylase; TAIR: AT2G36490.1 demeter-like 1; Swiss-Prot: sp|Q9SJQ6|ROS1_ARATH Protein ROS1; TrEMBL-Plants: tr|A0A0B2QF73|A0A0B2QF73_GLYSO Protein ROS1; Found in the gene: LotjaGi1g1v0679900 | 18 | ||
| – | DNA glycosylase; TAIR: AT5G04560.1 HhH-GPD base excision DNA repair family protein; Swiss-Prot: sp|Q8LK56|DME_ARATH Transcriptional activator DEMETER; TrEMBL-Plants: tr|A0A0R0EEG0|A0A0R0EEG0_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0156400_LC | 12 | ||
| – | DNA glycosylase; TAIR: AT5G04560.1 HhH-GPD base excision DNA repair family protein; Swiss-Prot: sp|Q8LK56|DME_ARATH Transcriptional activator DEMETER; TrEMBL-Plants: tr|A0A0R0EEG0|A0A0R0EEG0_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0156400_LC | 12 | ||
| – | DNA glycosylase; TAIR: AT5G04560.1 HhH-GPD base excision DNA repair family protein; Swiss-Prot: sp|Q8LK56|DME_ARATH Transcriptional activator DEMETER; TrEMBL-Plants: tr|A0A0R0EEG0|A0A0R0EEG0_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0156400_LC | 12 | ||
| – | DNA glycosylase; TAIR: AT5G04560.1 HhH-GPD base excision DNA repair family protein; Swiss-Prot: sp|Q8LK56|DME_ARATH Transcriptional activator DEMETER; TrEMBL-Plants: tr|A0A0R0EEG0|A0A0R0EEG0_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0156400_LC | 12 | ||
| – | DNA glycosylase; TAIR: AT5G04560.1 HhH-GPD base excision DNA repair family protein; Swiss-Prot: sp|Q8LK56|DME_ARATH Transcriptional activator DEMETER; TrEMBL-Plants: tr|A0A151RHD0|A0A151RHD0_CAJCA Transcriptional activator DEMETER; Found in the gene: LotjaGi2g1v0156400_LC | 12 | ||
| – | Endonuclease III homolog; TAIR: AT2G31450.2 DNA glycosylase superfamily protein; Swiss-Prot: sp|Q9SIC4|NTH1_ARATH Endonuclease III homolog 1, chloroplastic; TrEMBL-Plants: tr|A0A0L9V790|A0A0L9V790_PHAAN Endonuclease III homolog; Found in the gene: LotjaGi3g1v0497800 | 21 | ||
| – | DNA glycosylase; TAIR: AT2G36490.1 demeter-like 1; Swiss-Prot: sp|Q9SJQ6|ROS1_ARATH Protein ROS1; TrEMBL-Plants: tr|K7LHS4|K7LHS4_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0090900_LC | 18 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 7.69 |