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IPR014381

Description

IPR014381 is a DNA-directed RNA polymerase subunit Rpo5/Rpb5.

<p>Eukaryotic Rpb5 (POLR2E in mammals) is a common component of RNA polymerases I, II and III. Archaeal Rpo5 (also known as subunit H) is a homologue of Rpb5 [[cite:PUB00059148]]. Rpb5 has a bipartite structure consisting of a unique N-terminal region, plus a C-terminal region that is structurally homologous to Rpo5 [[cite:PUB00008456], [cite:PUB00003393], [cite:PUB00004780], [cite:PUB00008455]].</p> <p>DNA-directed RNA polymerases [ec:2.7.7.6] (also known as DNA-dependent RNA polymerases) are responsible for the polymerisation of ribonucleotides into a sequence complementary to the template DNA. In eukaryotes, there are three different forms of DNA-directed RNA polymerases transcribing different sets of genes. Most RNA polymerases are multimeric enzymes and are composed of a variable number of subunits. The core RNA polymerase complex consists of five subunits (two alpha, one beta, one beta-prime and one omega) and is sufficient for transcription elongation and termination but is unable to initiate transcription. Transcription initiation from promoter elements requires a sixth, dissociable subunit called a sigma factor, which reversibly associates with the core RNA polymerase complex to form a holoenzyme [[cite:PUB00000061]]. The core RNA polymerase complex forms a "crab claw"-like structure with an internal channel running along the full length [[cite:PUB00033173]]. The key functional sites of the enzyme, as defined by mutational and cross-linking analysis, are located on the inner wall of this channel.</p> <p>RNA synthesis follows after the attachment of RNA polymerase to a specific site, the promoter, on the template DNA strand. The RNA synthesis process continues until a termination sequence is reached. The RNA product, which is synthesised in the 5' to 3' direction, is known as the primary transcript.</p> <p>Eukaryotic nuclei contain three distinct types of RNA polymerases that differ in the RNA they synthesise:</p> <ul> <li>RNA polymerase I: located in the nucleoli, synthesises precursors of most ribosomal RNAs.</li> <li>RNA polymerase II: occurs in the nucleoplasm, synthesises mRNA precursors.</li> <li>RNA polymerase III: also occurs in the nucleoplasm, synthesises the precursors of 5S ribosomal RNA, the tRNAs, and a variety of other small nuclear and cytosolic RNAs.</li> </ul> <p>Eukaryotic cells are also known to contain separate mitochondrial and chloroplast RNA polymerases. Eukaryotic RNA polymerases, whose molecular masses vary in size from 500 to 700kDa, contain two non-identical large (>100kDa) subunits and an array of up to 12 different small (less than 50kDa) subunits.</p>

This description is obtained from EB-eye REST.

Associated GO terms

Unable to find any GO terms for the transcript with the identifier.

Associated Lotus transcripts 9

Transcript Name Description Predicted domains Domain count
RNA polymerase subunit, RPB5; RNA polymerase Rpb5, N-terminal [Medicago truncatula] gi|87162511|gb|ABD28306.1| 13
PREDICTED: DNA-directed RNA polymerases I, II, and III subunit RPABC1-like [Cicer arietinum] gi|502108221|ref|XP_004493418.1| 14
PREDICTED: DNA-directed RNA polymerases I, II, and III subunit RPABC1-like [Cicer arietinum] gi|502158924|ref|XP_004511328.1| 12
PREDICTED: DNA-directed RNA polymerases I, II, and III subunit RPABC1-like [Cicer arietinum] gi|502158924|ref|XP_004511328.1| 14
PREDICTED: DNA-directed RNA polymerases I, II, and III subunit RPABC1-like [Cicer arietinum] gi|502160738|ref|XP_004511874.1| 12
DNA-directed RNA polymerase II; TAIR: AT3G57080.1 Eukaryotic rpb5 RNA polymerase subunit family protein; Swiss-Prot: sp|Q9M1J2|RPE5A_ARATH DNA-directed RNA polymerase V subunit 5A; TrEMBL-Plants: tr|V7CXU1|V7CXU1_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0665900 16
DNA-directed RNA polymerase II; TAIR: AT3G22320.1 Eukaryotic rpb5 RNA polymerase subunit family protein; Swiss-Prot: sp|O81098|RPB5A_ARATH DNA-directed RNA polymerases II and IV subunit 5A; TrEMBL-Plants: tr|I3SCP2|I3SCP2_LOTJA Uncharacterized protein; Found in the gene: LotjaGi2g1v0219500 17
DNA-directed RNA polymerase II, putative; TAIR: AT3G54490.1 RNA polymerase II fifth largest subunit, E; Swiss-Prot: sp|Q9M1H8|RPE5C_ARATH DNA-directed RNA polymerase V subunit 5C; TrEMBL-Plants: tr|Q2HW77|Q2HW77_MEDTR RNA polymerase subunit, RPB5; RNA polymerase Rpb5, N-terminal; Found in the gene: LotjaGi3g1v0112400 15
DNA-directed RNA polymerase II; TAIR: AT3G22320.1 Eukaryotic rpb5 RNA polymerase subunit family protein; Swiss-Prot: sp|O81098|RPB5A_ARATH DNA-directed RNA polymerases II and IV subunit 5A; TrEMBL-Plants: tr|C6SVA4|C6SVA4_SOYBN Putative uncharacterized protein; Found in the gene: LotjaGi4g1v0457100 15

Co-occuring domains 1

A list of co-occurring predicted domains within the L. japonicus gene space:

Predicted domain Source Observations Saturation (%)
SSF55287 SUPERFAMILY 1 11.11