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IPR019810 is a Citrate synthase active site.
<p>Citrate synthase [ec:2.3.3.1] is a member of a small family of enzymes that can directly form a carbon-carbon bond without the presence of metal ion cofactors. It catalyses the first reaction in the Krebs' cycle, namely the conversion of oxaloacetate and acetyl-coenzyme A into citrate and coenzyme A. This reaction is important for energy generation and for carbon assimilation. The reaction proceeds via a non-covalently bound citryl-coenzyme A intermediate in a 2-step process (aldol-Claisen condensation followed by the hydrolysis of citryl-CoA).</p> <p>This entry represents types I and II citrate synthase enzymes, as well as the related enzymes 2-methylcitrate synthase and ATP citrate synthase. 2-methylcitrate ([ec:2.3.3.5]) synthase catalyses the conversion of oxaloacetate and propanoyl-CoA into (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate and coenzyme A. This enzyme is induced during bacterial and fungal growth on propionate [[cite:PUB00094532], [cite:PUB00090961]], while type II hexameric citrate synthase is constitutive [[cite:PUB00013490]]. ATP citrate synthase ([ec:2.3.3.8]) (also known as ATP citrate lyase) catalyses the MgATP-dependent, CoA-dependent cleavage of citrate into oxaloacetate and acetyl-CoA, a key step in the reductive tricarboxylic acid pathway of CO2 assimilation used by a variety of autotrophic bacteria and archaea to fix carbon dioxide [[cite:PUB00042606]]. ATP citrate synthase is composed of two distinct subunits. In eukaryotes, ATP citrate synthase is a homotetramer of a single large polypeptide, and is used to produce cytosolic acetyl-CoA from mitochondrial produced citrate [[cite:PUB00042607]]. This entry includes citrate synthase from Thermosulfidibacter takaii, which catalyses both citrate generation and citrate cleavage as it is part of a reversible tricarboxylic acid (TCA) cycle that can fix carbon dioxide autotrophically and may represent an ancestral mode of the conventional reductive TCA (rTCA) cycle [[cite:PUB00094533]].</p> <p>There are a number of regions of sequence similarity between prokaryotic and eukaryotic citrate synthases. One of the best conserved contains a histidine which is one of three residues shown to be involved in the catalytic mechanism of the vertebrate mitochondrial enzyme [[cite:PUB00000321]]. This entry represents this region.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | Tricarboxylic acid cycle | A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle. | ||
| Molecular function | Transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer | Catalysis of the transfer of an acyl group from one compound (donor) to another (acceptor), with the acyl group being converted into alkyl on transfer. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Citrate synthase [Medicago truncatula] gi|357494363|ref|XP_003617470.1| | 18 | ||
| – | PREDICTED: citrate synthase, mitochondrial-like [Glycine max] gi|356525687|ref|XP_003531455.1| | 13 | ||
| – | Citrate synthase [Medicago truncatula] gi|357459271|ref|XP_003599916.1| | 13 | ||
| – | Citrate synthase; TAIR: AT2G42790.1 citrate synthase 3; Swiss-Prot: sp|P49299|CYSZ_CUCMA Citrate synthase, glyoxysomal; TrEMBL-Plants: tr|G7K3F1|G7K3F1_MEDTR Citrate synthase; Found in the gene: LotjaGi1g1v0038100 | 19 | ||
| – | Citrate synthase; TAIR: AT2G44350.1 Citrate synthase family protein; Swiss-Prot: sp|P49298|CISY_CITMA Citrate synthase, mitochondrial; TrEMBL-Plants: tr|A0A0L9UJS8|A0A0L9UJS8_PHAAN Citrate synthase; Found in the gene: LotjaGi3g1v0127400 | 16 | ||
| – | Citrate synthase; TAIR: AT2G44350.1 Citrate synthase family protein; Swiss-Prot: sp|P49298|CISY_CITMA Citrate synthase, mitochondrial; TrEMBL-Plants: tr|A0A072V8L1|A0A072V8L1_MEDTR Citrate synthase; Found in the gene: LotjaGi3g1v0127400 | 20 | ||
| – | Citrate synthase; TAIR: AT2G42790.1 citrate synthase 3; Swiss-Prot: sp|P49299|CYSZ_CUCMA Citrate synthase, glyoxysomal; TrEMBL-Plants: tr|G7IZY5|G7IZY5_MEDTR Citrate synthase; Found in the gene: LotjaGi6g1v0170700 | 14 | ||
| – | Citrate synthase; TAIR: AT2G42790.1 citrate synthase 3; Swiss-Prot: sp|P49299|CYSZ_CUCMA Citrate synthase, glyoxysomal; TrEMBL-Plants: tr|G7IZY5|G7IZY5_MEDTR Citrate synthase; Found in the gene: LotjaGi6g1v0170700 | 19 | ||
| – | Citrate synthase; TAIR: AT2G42790.1 citrate synthase 3; Swiss-Prot: sp|P49299|CYSZ_CUCMA Citrate synthase, glyoxysomal; TrEMBL-Plants: tr|A0A151RYN3|A0A151RYN3_CAJCA Citrate synthase; Found in the gene: LotjaGi6g1v0170700 | 15 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| SSF48256 | SUPERFAMILY | 1 | 11.11 |