Your browser is unable to support new features implemented in HTML5 and CSS3 to render this site as intended. Your experience may suffer from functionality degradation but the site should remain usable. We strongly recommend the latest version of Google Chrome, OS X Safari or Mozilla Firefox. As Safari is bundled with OS X, if you are unable to upgrade to a newer version of OS X, we recommend using an open source browser. Dismiss message
IPR001884 is a Translation elongation factor IF5A-like.
<p>Eukaryotic eIF-5A was initially thought to function as a translation initiation factor, based on its ability to stimulate methionyl-puromycin synthesis. However, subsequent work revealed a role for eIF5A in translation elongation [[cite:PUB00046010], [cite:PUB00090482]]. Depletion or inactivation of eIF-5A in the yeast Saccharomyces cerevisiae (Baker's yeast) resulted in the accumulation of polysomes and an increase in ribosomal transit times. Addition of recombinant eIF-5A from yeast, but not a derivative lacking hypusine, enhanced the rate of tripeptide synthesis in vitro. Moreover, inactivation of eIF-5A mimicked the effects of the eEF2 inhibitor sordarin, indicating that eIF-5A might function together with eEF2 to promote ribosomal translocation. Finally, it was shown that eIF5A is specifically required to promote peptide-bond formation between consecutive proline residues. It has been proposed to stimulate the peptidyl-transferase activity of the ribosome and facilitate the reactivity of poor substrates like proline [[cite:PUB00082267]].</p> <p>eIF-5A is a cofactor for the Rev and Rex transactivator proteins of human immunodeficiency virus-1 and T-cell leukaemia virus I, respectively [[cite:PUB00000742], [cite:PUB00003672], [cite:PUB00010716]]. IF-5A is the sole protein in eukaryotes and archaea to contain the unusual amino acid hypusine (Ne-(4-amino-2-hydroxybutyl)lysine) that is an absolute functional requirement. The first step in the post-translational modification of lysine to hypusine is catalyzed by the enzyme deoxyhypusine synthase, the structure of which has been reported [[cite:PUB00005808]].</p> <p>This family also includes the Woronin body major protein Hex1, whose sequence and structure are similar to eukaryotic initiation factor 5A (eIF5A), suggesting they share a common ancestor during evolution [[cite:PUB00026734]]. Woronin bodies are important for stress resistance and virulence [[cite:PUB00101067]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | RNA binding | Interacting selectively and non-covalently with an RNA molecule or a portion thereof. | ||
| Molecular function | Translation elongation factor activity | Functions in chain elongation during polypeptide synthesis at the ribosome. | ||
| Molecular function | Ribosome binding | Interacting selectively and non-covalently with any part of a ribosome. | ||
| Biological process | Positive regulation of translational elongation | Any process that activates or increases the frequency, rate or extent of translational elongation. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Eukaryotic translation initiation factor 5A3 [Glycine max] gi|351721220|ref|NP_001237458.1| | 19 | ||
| – | PREDICTED: eukaryotic translation initiation factor 5A-4-like [Cicer arietinum] gi|502126980|ref|XP_004499516.1| | 19 | ||
| – | PREDICTED: eukaryotic translation initiation factor 5A-2-like [Cicer arietinum] gi|502149980|ref|XP_004507734.1| | 19 | ||
| – | Eukaryotic translation initiation factor 5A; TAIR: AT1G69410.1 eukaryotic elongation factor 5A-3; Swiss-Prot: sp|P24922|IF5A2_NICPL Eukaryotic translation initiation factor 5A-2; TrEMBL-Plants: tr|I3SDR1|I3SDR1_LOTJA Eukaryotic translation initiation factor 5A; Found in the gene: LotjaGi1g1v0193500 | 19 | ||
| – | Eukaryotic translation initiation factor 5A; TAIR: AT1G13950.1 eukaryotic elongation factor 5A-1; Swiss-Prot: sp|Q945F4|IF5A2_MEDSA Eukaryotic translation initiation factor 5A-2; TrEMBL-Plants: tr|I3T1J7|I3T1J7_LOTJA Eukaryotic translation initiation factor 5A; Found in the gene: LotjaGi2g1v0299200 | 19 | ||
| – | Eukaryotic translation initiation factor 5A; TAIR: AT1G13950.1 eukaryotic elongation factor 5A-1; Swiss-Prot: sp|Q9AXQ4|IF5A3_SOLLC Eukaryotic translation initiation factor 5A-3; TrEMBL-Plants: tr|I3SPM6|I3SPM6_LOTJA Eukaryotic translation initiation factor 5A; Found in the gene: LotjaGi4g1v0252100 | 18 | ||
| – | Eukaryotic translation initiation factor 5A; TAIR: AT1G13950.1 eukaryotic elongation factor 5A-1; Swiss-Prot: sp|P56336|IF5A4_SOLTU Eukaryotic translation initiation factor 5A-4; TrEMBL-Plants: tr|I3SPM6|I3SPM6_LOTJA Eukaryotic translation initiation factor 5A; Found in the gene: LotjaGi4g1v0252100 | 19 | ||
| – | Eukaryotic translation initiation factor 5A; TAIR: AT1G13950.1 eukaryotic elongation factor 5A-1; Swiss-Prot: sp|Q9AXJ4|IF5A_MANES Eukaryotic translation initiation factor 5A; TrEMBL-Plants: tr|I3SPM6|I3SPM6_LOTJA Eukaryotic translation initiation factor 5A; Found in the gene: LotjaGi4g1v0252100 | 13 | ||
| – | Eukaryotic translation initiation factor 5A; TAIR: AT2G24230.1 Leucine-rich repeat protein kinase family protein; Swiss-Prot: sp|C0LGK9|Y2242_ARATH Probable LRR receptor-like serine/threonine-protein kinase At2g24230; TrEMBL-Plants: tr|A0A0R0FIA7|A0A0R0FIA7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0287700 | 32 | ||
| – | Eukaryotic translation initiation factor 5A; TAIR: AT1G13950.1 eukaryotic elongation factor 5A-1; Swiss-Prot: sp|Q945F4|IF5A2_MEDSA Eukaryotic translation initiation factor 5A-2; TrEMBL-Plants: tr|I1JGP5|I1JGP5_SOYBN Eukaryotic translation initiation factor 5A; Found in the gene: LotjaGi4g1v0287700 | 19 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 10.00 |