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IPR002646 is a Poly A polymerase, head domain.
<p>This group includes nucleic acid independent RNA polymerases, such as polynucleotide adenylyltransferase ([ec:2.7.7.19]), which adds the poly (A) tail to mRNA. This group also includes the tRNA nucleotidyltransferase that adds the CCA to the 3' of the tRNA ([ec:2.7.7.25]).</p> <p>CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)]., one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction [[cite:PUB00081108], [cite:PUB00081109]]. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase [[cite:PUB00015829]], Escherichia coli poly(A) polymerase I [[cite:PUB00081110], [cite:PUB00081111]], human mitochondrial CCAase [[cite:PUB00022380], [cite:PUB00081120]], and Saccharomyces cerevisiae CCAase (CCA1) [[cite:PUB00081117], [cite:PUB00081118], [cite:PUB00081119]]. CCA-adding enzymes have a single catalytic pocket, which recognizes both ATP and CTP substrates [[cite:PUB00058171], [cite:PUB00081112]]. This family belongs to the Pol beta-like NT superfamily [[cite:PUB00013566], [cite:PUB00005745]].</p> <p>In the Pol beta-like NT superfamily [[cite:PUB00013566], [cite:PUB00005745]], the majority of enzymes have two carboxylates, Dx[D/E], together with a third more distal carboxylate, which coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | RNA binding | Interacting selectively and non-covalently with an RNA molecule or a portion thereof. | ||
| Biological process | RNA processing | Any process involved in the conversion of one or more primary RNA transcripts into one or more mature RNA molecules. | ||
| Molecular function | Nucleotidyltransferase activity | Catalysis of the transfer of a nucleotidyl group to a reactant. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | TRNA nucleotidyltransferase [Lupinus albus] gi|1139585|gb|AAB03077.1| | 11 | ||
| – | PREDICTED: CCA-adding enzyme-like [Cicer arietinum] gi|502133372|ref|XP_004501740.1| | 9 | ||
| – | PREDICTED: CCA-adding enzyme-like [Cicer arietinum] gi|502133372|ref|XP_004501740.1| | 11 | ||
| – | Poly(A) polymerase [Medicago truncatula] gi|357484075|ref|XP_003612324.1| | 12 | ||
| – | PREDICTED: poly(A) polymerase-like [Cicer arietinum] gi|502139505|ref|XP_004503795.1| | 14 | ||
| – | PREDICTED: CCA tRNA nucleotidyltransferase, mitochondrial-like [Glycine max] gi|356576137|ref|XP_003556190.1| | 11 | ||
| – | Multifunctional CCA protein; TAIR: AT1G22660.1 Polynucleotide adenylyltransferase family protein; Swiss-Prot: sp|P21269|CCA1_YEAST CCA tRNA nucleotidyltransferase, mitochondrial; TrEMBL-Plants: tr|I1NH95|I1NH95_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0094900 | 8 | ||
| – | Multifunctional CCA protein; TAIR: AT3G48830.1 tRNA nucleotidyltransferase/polyA polymerase family protein; Swiss-Prot: sp|P0ABF3|PCNB_ECO57 Poly(A) polymerase I; TrEMBL-Plants: tr|K7KL92|K7KL92_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0334300 | 12 | ||
| – | Multifunctional CCA protein; TAIR: AT2G17580.1 Polynucleotide adenylyltransferase family protein; Swiss-Prot: sp|P44439|PCNB_HAEIN Poly(A) polymerase I; TrEMBL-Plants: tr|K7K447|K7K447_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0356000 | 13 | ||
| – | Multifunctional CCA protein; TAIR: AT3G48830.1 tRNA nucleotidyltransferase/polyA polymerase family protein; Swiss-Prot: sp|P44439|PCNB_HAEIN Poly(A) polymerase I; TrEMBL-Plants: tr|G7LI47|G7LI47_MEDTR tRNA nucleotidyltransferase/poly(A) polymerase; Found in the gene: LotjaGi4g1v0420500 | 15 | ||
| – | Multifunctional CCA protein; TAIR: AT3G48830.1 tRNA nucleotidyltransferase/polyA polymerase family protein; Swiss-Prot: sp|P44439|PCNB_HAEIN Poly(A) polymerase I; TrEMBL-Plants: tr|G7LI47|G7LI47_MEDTR tRNA nucleotidyltransferase/poly(A) polymerase; Found in the gene: LotjaGi4g1v0420500 | 15 | ||
| – | Multifunctional CCA protein; TAIR: AT1G22660.1 Polynucleotide adenylyltransferase family protein; Swiss-Prot: sp|Q9P4S5|CCA1_CANGA CCA tRNA nucleotidyltransferase, mitochondrial; TrEMBL-Plants: tr|I1NH95|I1NH95_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0281000 | 10 | ||
| – | TRNA nucleotidyltransferase (CCA-adding enzyme); TAIR: AT1G22660.3 Polynucleotide adenylyltransferase family protein; Swiss-Prot: sp|Q9P4S5|CCA1_CANGA CCA tRNA nucleotidyltransferase, mitochondrial; TrEMBL-Plants: tr|Q42867|Q42867_LUPAL tRNA nucleotidyltransferase; Found in the gene: LotjaGi6g1v0218500_LC | 11 | ||
| – | TRNA nucleotidyltransferase (CCA-adding enzyme); TAIR: AT1G22660.3 Polynucleotide adenylyltransferase family protein; Swiss-Prot: sp|Q9P4S5|CCA1_CANGA CCA tRNA nucleotidyltransferase, mitochondrial; TrEMBL-Plants: tr|Q42867|Q42867_LUPAL tRNA nucleotidyltransferase; Found in the gene: LotjaGi6g1v0218500_LC | 11 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| SSF81301 | SUPERFAMILY | 1 | 7.14 |