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IPR004522 is a Asparagine-tRNA ligase.
<p>Asparagine tRNA ligase ([ec:6.1.1.22]) is an alpha2 dimer that belongs to class IIb. There is a striking similarity between asparagine-tRNA ligases and archaeal/eukaryotic type aspartyl-tRNA ligases ([interpro:IPR004523]) and a striking divergence of bacterial type aspartyl-tRNA ligases ([interpro:IPR004524]). This family, AsnS, represents asparagine-tRNA ligases from the three domains of life. Some species lack this enzyme and charge tRNA(asn) by misacylation with Asp, followed by transamidation of Asp to Asn.</p> <p>The aminoacyl-tRNA synthetases (also known as aminoacyl-tRNA ligases) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction [[cite:PUB00079872], [cite:PUB00079873]]. These proteins differ widely in size and oligomeric state, and have limited sequence homology [[cite:PUB00007191]]. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric [[cite:PUB00006477]]. Class II aminoacyl-tRNA synthetases share an anti-parallel β-sheet fold flanked by α-helices [[cite:PUB00000386]], and are mostly dimeric or multimeric, containing at least three conserved regions [[cite:PUB00000723], [cite:PUB00005365], [cite:PUB00004391]]. However, tRNA binding involves an α-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan, valine, and some lysine synthetases (non-eukaryotic group) belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, phenylalanine, proline, serine, threonine, and some lysine synthetases (non-archaeal group), belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c [[cite:PUB00007363]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Nucleotide binding | Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose. | ||
| Molecular function | Asparagine-tRNA ligase activity | Catalysis of the reaction: L-asparagine + ATP + tRNA(Asn) = AMP + Asn-tRNA(Asn) + diphosphate + 2 H(+). | ||
| Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
| Biological process | Asparaginyl-tRNA aminoacylation | The process of coupling asparagine to asparaginyl-tRNA, catalyzed by asparaginyl-tRNA synthetase. The asparaginyl-tRNA synthetase is a class-II synthetase. The activated amino acid is transferred to the 3'-OH group of an asparagine-accetping tRNA. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: asparagine--tRNA ligase, cytoplasmic 1-like [Cicer arietinum] gi|502138744|ref|XP_004503515.1| | 18 | ||
| – | PREDICTED: asparagine--tRNA ligase, chloroplastic/mitochondrial-like [Cicer arietinum] gi|502152388|ref|XP_004508904.1| | 17 | ||
| – | PREDICTED: asparagine--tRNA ligase, chloroplastic/mitochondrial-like [Cicer arietinum] gi|502152388|ref|XP_004508904.1| | 22 | ||
| – | PREDICTED: asparagine--tRNA ligase, cytoplasmic 1-like [Cicer arietinum] gi|502138744|ref|XP_004503515.1| | 28 | ||
| – | Asparagine-tRNA ligase; TAIR: AT4G17300.2 Class II aminoacyl-tRNA and biotin synthetases superfamily protein; Swiss-Prot: sp|O48593|SYNO_ARATH Asparagine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|G7JIW4|G7JIW4_MEDTR Asparagine-tRNA ligase; Found in the gene: LotjaGi4g1v0203000 | 18 | ||
| – | Asparagine--tRNA ligase; TAIR: AT5G56680.1 Class II aminoacyl-tRNA and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9SW96|SYNC1_ARATH Asparagine--tRNA ligase, cytoplasmic 1; TrEMBL-Plants: tr|V7CQ72|V7CQ72_PHAVU Uncharacterized protein; Found in the gene: LotjaGi4g1v0442400 | 25 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd04318 | CDD | 1 | 16.67 |