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IPR004364 is a Aminoacyl-tRNA synthetase, class II (D/K/N).
<p>The aminoacyl-tRNA synthetases (also known as aminoacyl-tRNA ligases) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction [[cite:PUB00079872], [cite:PUB00079873]]. These proteins differ widely in size and oligomeric state, and have limited sequence homology [[cite:PUB00007191]]. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric [[cite:PUB00006477]]. Class II aminoacyl-tRNA synthetases share an anti-parallel β-sheet fold flanked by α-helices [[cite:PUB00000386]], and are mostly dimeric or multimeric, containing at least three conserved regions [[cite:PUB00000723], [cite:PUB00005365], [cite:PUB00004391]]. However, tRNA binding involves an α-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan, valine, and some lysine synthetases (non-eukaryotic group) belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, phenylalanine, proline, serine, threonine, and some lysine synthetases (non-archaeal group), belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c [[cite:PUB00007363]].</p> <p>This entry includes the asparagine, aspartic acid and lysine tRNA synthetases.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
GO term | Namespace | Name | Definition | Relationships |
---|---|---|---|---|
Molecular function | Nucleotide binding | Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose. | ||
Molecular function | Aminoacyl-tRNA ligase activity | Catalysis of the formation of aminoacyl-tRNA from ATP, amino acid, and tRNA with the release of diphosphate and AMP. | ||
Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
Biological process | TRNA aminoacylation for protein translation | The synthesis of aminoacyl tRNA by the formation of an ester bond between the 3'-hydroxyl group of the most 3' adenosine of the tRNA and the alpha carboxylic acid group of an amino acid, to be used in ribosome-mediated polypeptide synthesis. |
Transcript | Name | Description | Predicted domains | Domain count |
---|---|---|---|---|
– | PREDICTED: asparagine--tRNA ligase, cytoplasmic 1-like [Cicer arietinum] gi|502138744|ref|XP_004503515.1| | 7 | ||
– | PREDICTED: lysyl-tRNA synthetase-like [Glycine max] gi|356533832|ref|XP_003535462.1| | 19 | ||
– | Asparaginyl-tRNA synthetase [Medicago truncatula] gi|357513179|ref|XP_003626878.1| | 9 | ||
– | PREDICTED: aspartate--tRNA ligase, cytoplasmic-like [Cicer arietinum] gi|502162327|ref|XP_004512467.1| | 17 | ||
– | PREDICTED: lysyl-tRNA synthetase-like [Glycine max] gi|356504551|ref|XP_003521059.1| | 23 | ||
– | PREDICTED: aspartate--tRNA ligase, cytoplasmic-like [Cicer arietinum] gi|502125110|ref|XP_004498802.1| | 20 | ||
– | PREDICTED: asparagine--tRNA ligase, cytoplasmic 1-like [Cicer arietinum] gi|502138744|ref|XP_004503515.1| | 18 | ||
– | PREDICTED: asparagine--tRNA ligase, chloroplastic/mitochondrial-like [Cicer arietinum] gi|502152388|ref|XP_004508904.1| | 17 | ||
– | PREDICTED: asparagine--tRNA ligase, chloroplastic/mitochondrial-like [Cicer arietinum] gi|502152388|ref|XP_004508904.1| | 22 | ||
– | PREDICTED: lysyl-tRNA synthetase-like [Glycine max] gi|356533832|ref|XP_003535462.1| | 24 | ||
– | PREDICTED: asparagine--tRNA ligase, cytoplasmic 1-like [Cicer arietinum] gi|502138744|ref|XP_004503515.1| | 28 | ||
– | PREDICTED: aspartate--tRNA ligase-like isoform X1 [Cicer arietinum] gi|502138374|ref|XP_004503382.1| | 31 | ||
– | PREDICTED: aspartate--tRNA ligase-like isoform X1 [Cicer arietinum] gi|502138374|ref|XP_004503382.1| | 14 | ||
– | PREDICTED: aspartate--tRNA ligase, cytoplasmic-like [Cicer arietinum] gi|502125110|ref|XP_004498802.1| | 20 | ||
– | Lysine--tRNA ligase; TAIR: AT3G13490.1 Lysyl-tRNA synthetase, class II; Swiss-Prot: sp|Q9LJE2|SYKM_ARATH Lysine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A151TGE1|A0A151TGE1_CAJCA Lysine--tRNA ligase; Found in the gene: LotjaGi2g1v0226400 | 22 | ||
– | Lysine--tRNA ligase; TAIR: AT3G13490.1 Lysyl-tRNA synthetase, class II; Swiss-Prot: sp|Q9LJE2|SYKM_ARATH Lysine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A072TKF3|A0A072TKF3_MEDTR Lysine--tRNA ligase; Found in the gene: LotjaGi2g1v0226400 | 13 | ||
– | Lysine--tRNA ligase; TAIR: AT3G13490.1 Lysyl-tRNA synthetase, class II; Swiss-Prot: sp|Q9LJE2|SYKM_ARATH Lysine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A072TKF3|A0A072TKF3_MEDTR Lysine--tRNA ligase; Found in the gene: LotjaGi2g1v0226400 | 15 | ||
– | Asparagine-tRNA ligase; TAIR: AT3G07420.1 asparaginyl-tRNA synthetase 2; Swiss-Prot: sp|Q9SW95|SYNC2_ARATH Asparagine--tRNA ligase, cytoplasmic 2; TrEMBL-Plants: tr|G7LED0|G7LED0_MEDTR Asparagine-tRNA ligase; Found in the gene: LotjaGi3g1v0268500 | 7 | ||
– | Asparagine--tRNA ligase; TAIR: AT5G56680.1 Class II aminoacyl-tRNA and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9SW96|SYNC1_ARATH Asparagine--tRNA ligase, cytoplasmic 1; TrEMBL-Plants: tr|I1K622|I1K622_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0417100 | 10 | ||
– | Asparagine-tRNA ligase; TAIR: AT4G17300.2 Class II aminoacyl-tRNA and biotin synthetases superfamily protein; Swiss-Prot: sp|O48593|SYNO_ARATH Asparagine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|G7JIW4|G7JIW4_MEDTR Asparagine-tRNA ligase; Found in the gene: LotjaGi4g1v0203000 | 18 | ||
– | Asparagine-tRNA ligase; TAIR: AT4G17300.1 Class II aminoacyl-tRNA and biotin synthetases superfamily protein; Swiss-Prot: sp|O48593|SYNO_ARATH Asparagine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|G7JIW4|G7JIW4_MEDTR Asparagine-tRNA ligase; Found in the gene: LotjaGi4g1v0203000 | 9 | ||
– | Lysine--tRNA ligase; TAIR: AT3G11710.1 lysyl-tRNA synthetase 1; Swiss-Prot: sp|Q9ZPI1|SYKC_ARATH Lysine--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1LZI3|I1LZI3_SOYBN Lysine--tRNA ligase; Found in the gene: LotjaGi4g1v0302800 | 24 | ||
– | Lysine--tRNA ligase; TAIR: AT3G11710.1 lysyl-tRNA synthetase 1; Swiss-Prot: sp|Q9ZPI1|SYKC_ARATH Lysine--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1L9B1|I1L9B1_SOYBN Lysine--tRNA ligase; Found in the gene: LotjaGi4g1v0302800 | 24 | ||
– | Lysine--tRNA ligase; TAIR: AT3G11710.1 lysyl-tRNA synthetase 1; Swiss-Prot: sp|Q6F2U9|SYK_ORYSJ Lysine--tRNA ligase; TrEMBL-Plants: tr|A0A1J7IH24|A0A1J7IH24_LUPAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0302800 | 13 | ||
– | Asparagine--tRNA ligase; TAIR: AT5G56680.1 Class II aminoacyl-tRNA and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9SW96|SYNC1_ARATH Asparagine--tRNA ligase, cytoplasmic 1; TrEMBL-Plants: tr|I1K622|I1K622_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0442400 | 10 | ||
– | Asparagine--tRNA ligase; TAIR: AT5G56680.1 Class II aminoacyl-tRNA and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9SW96|SYNC1_ARATH Asparagine--tRNA ligase, cytoplasmic 1; TrEMBL-Plants: tr|V7CQ72|V7CQ72_PHAVU Uncharacterized protein; Found in the gene: LotjaGi4g1v0442400 | 25 | ||
– | Aspartate--tRNA ligase; TAIR: AT4G33760.1 tRNA synthetase class II (D, K and N) family protein; Swiss-Prot: sp|F4JJT9|SYDM_ARATH Aspartate--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A151T3C0|A0A151T3C0_CAJCA Aspartyl-tRNA synthetase; Found in the gene: LotjaGi4g1v0454600 | 30 | ||
– | Aspartate--tRNA ligase; TAIR: AT4G33760.1 tRNA synthetase class II (D, K and N) family protein; Swiss-Prot: sp|F4JJT9|SYDM_ARATH Aspartate--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A151T3C0|A0A151T3C0_CAJCA Aspartyl-tRNA synthetase; Found in the gene: LotjaGi4g1v0454600 | 28 | ||
– | Aspartate-tRNA ligase; TAIR: AT4G31180.1 Class II aminoacyl-tRNA and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9M084|SYDC2_ARATH Aspartate--tRNA ligase 2, cytoplasmic; TrEMBL-Plants: tr|I1M984|I1M984_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0052700 | 20 | ||
– | Aspartate-tRNA ligase; TAIR: AT4G31180.1 Class II aminoacyl-tRNA and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9M084|SYDC2_ARATH Aspartate--tRNA ligase 2, cytoplasmic; TrEMBL-Plants: tr|A0A0L9V226|A0A0L9V226_PHAAN Uncharacterized protein; Found in the gene: LotjaGi5g1v0052700 | 11 | ||
– | Lysine--tRNA ligase; TAIR: AT3G11710.1 lysyl-tRNA synthetase 1; Swiss-Prot: sp|Q9ZPI1|SYKC_ARATH Lysine--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1LZI3|I1LZI3_SOYBN Lysine--tRNA ligase; Found in the gene: LotjaGi6g1v0240700 | 30 | ||
– | Lysine--tRNA ligase; TAIR: AT3G11710.1 lysyl-tRNA synthetase 1; Swiss-Prot: sp|Q9ZPI1|SYKC_ARATH Lysine--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1LZI3|I1LZI3_SOYBN Lysine--tRNA ligase; Found in the gene: LotjaGi6g1v0240700 | 22 | ||
– | Lysine--tRNA ligase; TAIR: AT3G11710.1 lysyl-tRNA synthetase 1; Swiss-Prot: sp|Q9ZPI1|SYKC_ARATH Lysine--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1LZI3|I1LZI3_SOYBN Lysine--tRNA ligase; Found in the gene: LotjaGi6g1v0240700 | 24 | ||
– | Lysine--tRNA ligase; TAIR: AT3G11710.1 lysyl-tRNA synthetase 1; Swiss-Prot: sp|Q6F2U9|SYK_ORYSJ Lysine--tRNA ligase; TrEMBL-Plants: tr|I1LZI3|I1LZI3_SOYBN Lysine--tRNA ligase; Found in the gene: LotjaGi6g1v0240700 | 11 | ||
– | Lysine--tRNA ligase; TAIR: AT3G11710.1 lysyl-tRNA synthetase 1; Swiss-Prot: sp|Q9ZPI1|SYKC_ARATH Lysine--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1LZI3|I1LZI3_SOYBN Lysine--tRNA ligase; Found in the gene: LotjaGi6g1v0240700 | 24 |
A list of co-occurring predicted domains within the L. japonicus gene space:
Predicted domain | Source | Observations | Saturation (%) |
---|---|---|---|
mobidb-lite | MobiDBLite | 1 | 2.86 |