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IPR005671 is a 2-isopropylmalate synthase, bacterial-type.
<p>The branched-chain amino acids are synthesised by a common pathway that leads from pyruvate and alpha-ketobutyrate to valine and isoleucine, and a branch that leads from the immediate precursor of valine, alpha-ketoisovalerate, to leucine [[cite:PUB00034699]]. This pathway operates in archaea, bacteria, fungi and plants, but not mammals, making the enzymes suitable targets for the development of novel antibiotics and herbicides.</p> <p>Isopropylmalate synthase is the enzyme responsible for the the first committed step in the leucine branch of this biosynthetic pathway, the conversion of alpha-ketoisovalerate to alpha-isopropylmalate. It is either dimeric or tetrameric, depending on the organism, with a monomer molecular mass of 60-70kDa, a dependence on divalent metal ions for activity, and an alkaline pH optimum [[cite:PUB00034700], [cite:PUB00034701], [cite:PUB00034702], [cite:PUB00020847]]. Like many other biosynthetic enzymes it is subject to feedback inhibition by the end product of the pathway, leucine.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | 2-isopropylmalate synthase activity | Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+). | ||
| Biological process | Leucine biosynthetic process | The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Homocitrate synthase [Lotus japonicus] gi|268607430|dbj|BAI49592.1| | 19 | ||
| – | PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cicer arietinum] gi|502121886|ref|XP_004497478.1| | 18 | ||
| – | 2-isopropylmalate synthase; TAIR: AT1G74040.1 2-isopropylmalate synthase 1; Swiss-Prot: sp|Q39891|LEU1_SOYBN Probable 2-isopropylmalate synthase; TrEMBL-Plants: tr|D0VY45|D0VY45_LOTJA Homocitrate synthase; Found in the gene: LotjaGi1g1v0613300 | 22 | ||
| – | 2-isopropylmalate synthase; TAIR: AT1G74040.1 2-isopropylmalate synthase 1; Swiss-Prot: sp|Q39891|LEU1_SOYBN Probable 2-isopropylmalate synthase; TrEMBL-Plants: tr|D0VY45|D0VY45_LOTJA Homocitrate synthase; Found in the gene: LotjaGi1g1v0613400 | 26 | ||
| – | 2-isopropylmalate synthase; TAIR: AT1G74040.1 2-isopropylmalate synthase 1; Swiss-Prot: sp|Q9C550|LEU12_ARATH 2-isopropylmalate synthase 2, chloroplastic; TrEMBL-Plants: tr|I1NJA5|I1NJA5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0362600 | 21 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd07940 | CDD | 1 | 20.00 |