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IPR000891 is a Pyruvate carboxyltransferase.
<p>Pyruvate carboxylase ([ec:6.4.1.1]) (PC), a member of the biotin-dependent enzyme family, is involved in gluconeogenesis by mediating the carboxylation of pyruvate to oxaloacetate. Biotin-dependent carboxylase enzymes perform a two step reaction. Enzyme-bound biotin is first carboxylated by bicarbonate and ATP and the carboxyl group temporarily bound to biotin is subsequently transferred to an acceptor substrate such as pyruvate [[cite:PUB00015343]]. PC has three functional domains: a biotin carboxylase (BC) domain, a carboxyltransferase (CT) domain which perform the second part of the reaction and a biotinyl domain [[cite:PUB00015403], [cite:PUB00015404]]. The pyruvate binding to the CT active site induces a conformational change stabilised by the interaction of conserved Asp and Tyr residues in this domain which leads to the formation of the biotin binding pocket and ensures the efficient coupling of BC and CT domain reactions [[cite:PUB00095137]]. The mechanism by which the carboxyl group is transferred from the carboxybiotin to the pyruvate is not well understood.</p> <p>The pyruvate carboxyltransferase domain is also found in other pyruvate binding enzymes and acetyl-CoA dependent enzymes suggesting that this domain can be associated with different enzymatic activities.</p> <p>This domain is found towards the N-terminal region of various aldolase enzymes. This N-terminal TIM barrel domain [[cite:PUB00016385]] interacts with the C-terminal domain. The C-terminal DmpG_comm domain ([interpro:IPR012425]) is thought to promote heterodimerization with members of [interpro:IPR003361] to form a bifunctional aldolase-dehydrogenase [[cite:PUB00016385]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Catalytic activity | Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Hydroxymethylglutaryl-CoA lyase [Medicago truncatula] gi|357458801|ref|XP_003599681.1| | 9 | ||
| – | PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Glycine max] gi|356521713|ref|XP_003529496.1| | 8 | ||
| – | Homocitrate synthase [Lotus japonicus] gi|268607430|dbj|BAI49592.1| | 16 | ||
| – | Homocitrate synthase [Lotus japonicus] gi|268607430|dbj|BAI49592.1| | 14 | ||
| – | Homocitrate synthase [Lotus japonicus] gi|268607430|dbj|BAI49592.1| | 12 | ||
| – | Homocitrate synthase [Lotus japonicus] gi|268607430|dbj|BAI49592.1| | 19 | ||
| – | Homocitrate synthase [Lotus japonicus] gi|268607430|dbj|BAI49592.1| | 13 | ||
| – | Homocitrate synthase [Lotus japonicus] gi|268607430|dbj|BAI49592.1| | 10 | ||
| – | PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Cicer arietinum] gi|502108072|ref|XP_004493382.1| | 14 | ||
| – | PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Cicer arietinum] gi|502108072|ref|XP_004493382.1| | 9 | ||
| – | PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cicer arietinum] gi|502121886|ref|XP_004497478.1| | 18 | ||
| – | 2-isopropylmalate synthase; TAIR: AT1G74040.1 2-isopropylmalate synthase 1; Swiss-Prot: sp|Q39891|LEU1_SOYBN Probable 2-isopropylmalate synthase; TrEMBL-Plants: tr|D0VY45|D0VY45_LOTJA Homocitrate synthase; Found in the gene: LotjaGi1g1v0613100 | 19 | ||
| – | 2-isopropylmalate synthase; TAIR: AT1G74040.1 2-isopropylmalate synthase 1; Swiss-Prot: sp|Q39891|LEU1_SOYBN Probable 2-isopropylmalate synthase; TrEMBL-Plants: tr|D0VY45|D0VY45_LOTJA Homocitrate synthase; Found in the gene: LotjaGi1g1v0613300 | 22 | ||
| – | 2-isopropylmalate synthase; TAIR: AT1G74040.1 2-isopropylmalate synthase 1; Swiss-Prot: sp|Q39891|LEU1_SOYBN Probable 2-isopropylmalate synthase; TrEMBL-Plants: tr|D0VY45|D0VY45_LOTJA Homocitrate synthase; Found in the gene: LotjaGi1g1v0613400 | 26 | ||
| – | 2-isopropylmalate synthase; TAIR: AT1G74040.1 2-isopropylmalate synthase 1; Swiss-Prot: sp|Q39891|LEU1_SOYBN Probable 2-isopropylmalate synthase; TrEMBL-Plants: tr|D0VY45|D0VY45_LOTJA Homocitrate synthase; Found in the gene: LotjaGi1g1v0613400 | 24 | ||
| – | 2-isopropylmalate synthase 2; TAIR: AT2G26800.5 Aldolase superfamily protein; Swiss-Prot: sp|O81027|HMGCL_ARATH Hydroxymethylglutaryl-CoA lyase, mitochondrial; TrEMBL-Plants: tr|I1N8Q3|I1N8Q3_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0661300 | 15 | ||
| – | 2-isopropylmalate synthase 2; TAIR: AT2G26800.5 Aldolase superfamily protein; Swiss-Prot: sp|O81027|HMGCL_ARATH Hydroxymethylglutaryl-CoA lyase, mitochondrial; TrEMBL-Plants: tr|A0A0R0EW28|A0A0R0EW28_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0661300 | 10 | ||
| – | 2-isopropylmalate synthase; TAIR: AT1G74040.1 2-isopropylmalate synthase 1; Swiss-Prot: sp|Q9C550|LEU12_ARATH 2-isopropylmalate synthase 2, chloroplastic; TrEMBL-Plants: tr|I1NJA5|I1NJA5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0362600 | 21 | ||
| – | 2-isopropylmalate synthase; TAIR: AT1G74040.1 2-isopropylmalate synthase 1; Swiss-Prot: sp|Q9C550|LEU12_ARATH 2-isopropylmalate synthase 2, chloroplastic; TrEMBL-Plants: tr|I1NJA5|I1NJA5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0362600 | 15 | ||
| – | 2-isopropylmalate synthase; TAIR: AT2G26800.1 Aldolase superfamily protein; Swiss-Prot: sp|O81027|HMGCL_ARATH Hydroxymethylglutaryl-CoA lyase, mitochondrial; TrEMBL-Plants: tr|A0A151T0C4|A0A151T0C4_CAJCA Putative 3-hydroxymethyl-3-methylglutaryl-CoA lyase 2; Found in the gene: LotjaGi6g1v0177500 | 11 | ||
| – | 2-isopropylmalate synthase; TAIR: AT2G26800.1 Aldolase superfamily protein; Swiss-Prot: sp|O81027|HMGCL_ARATH Hydroxymethylglutaryl-CoA lyase, mitochondrial; TrEMBL-Plants: tr|A0A151T0C4|A0A151T0C4_CAJCA Putative 3-hydroxymethyl-3-methylglutaryl-CoA lyase 2; Found in the gene: LotjaGi6g1v0177500 | 11 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 4.76 |