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IPR006982 is a Glutamate synthase, central-N.
<p>The central domain of glutamate synthase connects the N-terminal amidotransferase domain with the FMN-binding domain and has an α/β overall topology [[cite:PUB00008698]].</p> <p>Glutamate synthase (GltS)1 is a key enzyme in the early stages of the assimilation of ammonia in bacteria, yeasts, and plants. In bacteria, L-glutamate is involved in osmoregulation, is the precursor for other amino acids, and can be the precursor for haem biosynthesis. In plants, GltS is especially essential in the reassimilation of ammonia released by photorespiration. On the basis of the amino acid sequence and the nature of the electron donor, three different classes of GltS can de defined as follows: 1) ferredoxin-dependent GltS (Fd-GltS), 2) NADPH-dependent GltS (NADPH-GltS), and 3) NADH-dependent GltS (properties of the three classes have been reviewed extensively [[cite:PUB00009386]]). The enzyme is a complex iron-sulphur flavoprotein catalysing the reductive transfer of the amido nitrogen from L-glutamine to 2-oxoglutarate to form two molecules of L-glutamate via intramolecular channelling of ammonia from the amidotransferase domain to the FMN-binding domain.</p> <p>Reaction of amidotransferase domain:</p> <p><reaction>L-glutamine + H2O = L-glutamate + NH3</reaction></p> <p>Reactions of FMN-binding domain:</p> <p><reaction>2-oxoglutarate + NH3 = 2-iminoglutarate + H2O</reaction></p> <p><reaction>2e + FMNox = FMNred</reaction></p> <p><reaction>2-iminoglutarate + FMNred = L-glutamate + FMNox</reaction></p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | Nitrogen compound metabolic process | The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen. | ||
| Molecular function | Glutamate synthase activity | Catalysis of the formation of L-glutamine and 2-oxoglutarate from L-glutamate, using NADH, NADPH or ferredoxin as hydrogen acceptors. | ||
| Biological process | Oxidation-reduction process | A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: glutamate synthase [NADH] gi|356571346|ref|XP_003553839.1| | 24 | ||
| – | PREDICTED: ferredoxin-dependent glutamate synthase 1, chloroplastic-like isoform X1 [Cicer arietinum] gi|502108182|ref|XP_004493407.1| | 21 | ||
| – | PREDICTED: ferredoxin-dependent glutamate synthase 1, chloroplastic-like isoform X1 [Cicer arietinum] gi|502108182|ref|XP_004493407.1| | 19 | ||
| – | PREDICTED: ferredoxin-dependent glutamate synthase 1, chloroplastic-like isoform X1 [Cicer arietinum] gi|502108182|ref|XP_004493407.1| | 11 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A0B2SWZ8|A0A0B2SWZ8_GLYSO Glutamate synthase [NADH], amyloplastic; Found in the gene: LotjaGi1g1v0224700 | 40 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A072VE66|A0A072VE66_MEDTR NADH glutamate synthase; Found in the gene: LotjaGi1g1v0224700 | 36 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A072VE66|A0A072VE66_MEDTR NADH glutamate synthase; Found in the gene: LotjaGi1g1v0224700 | 23 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A0B2SWZ8|A0A0B2SWZ8_GLYSO Glutamate synthase [NADH], amyloplastic; Found in the gene: LotjaGi1g1v0224700 | 40 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A072VE66|A0A072VE66_MEDTR NADH glutamate synthase; Found in the gene: LotjaGi1g1v0224700 | 45 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A0B2SWZ8|A0A0B2SWZ8_GLYSO Glutamate synthase [NADH], amyloplastic; Found in the gene: LotjaGi1g1v0224700 | 37 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A0B2SWZ8|A0A0B2SWZ8_GLYSO Glutamate synthase [NADH], amyloplastic; Found in the gene: LotjaGi1g1v0224700 | 40 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A0S3RN10|A0A0S3RN10_PHAAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0224700 | 40 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A0B2SLS9|A0A0B2SLS9_GLYSO Glutamate synthase [NADH], amyloplastic; Found in the gene: LotjaGi1g1v0224700 | 23 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A072VE66|A0A072VE66_MEDTR NADH glutamate synthase; Found in the gene: LotjaGi1g1v0224700 | 30 | ||
| – | Glutamate synthase, putative; TAIR: AT5G53460.1 NADH-dependent glutamate synthase 1; Swiss-Prot: sp|Q03460|GLSN_MEDSA Glutamate synthase [NADH], amyloplastic; TrEMBL-Plants: tr|A0A072VE66|A0A072VE66_MEDTR NADH glutamate synthase; Found in the gene: LotjaGi1g1v0224700 | 45 | ||
| – | Glutamate synthase, putative; TAIR: AT5G04140.1 glutamate synthase 1; Swiss-Prot: sp|Q43155|GLTB_SPIOL Ferredoxin-dependent glutamate synthase, chloroplastic; TrEMBL-Plants: tr|A0A151RUY7|A0A151RUY7_CAJCA Uncharacterized protein; Found in the gene: LotjaGi1g1v0664300 | 29 | ||
| – | Glutamate synthase, putative; TAIR: AT5G04140.1 glutamate synthase 1; Swiss-Prot: sp|Q43155|GLTB_SPIOL Ferredoxin-dependent glutamate synthase, chloroplastic; TrEMBL-Plants: tr|A0A151RUY7|A0A151RUY7_CAJCA Uncharacterized protein; Found in the gene: LotjaGi1g1v0664300 | 24 | ||
| – | Glutamate synthase, putative; TAIR: AT5G04140.1 glutamate synthase 1; Swiss-Prot: sp|Q43155|GLTB_SPIOL Ferredoxin-dependent glutamate synthase, chloroplastic; TrEMBL-Plants: tr|A0A0E0QC77|A0A0E0QC77_ORYRU Uncharacterized protein; Found in the gene: LotjaGi1g1v0664300 | 24 | ||
| – | Glutamate synthase, putative; TAIR: AT5G04140.1 glutamate synthase 1; Swiss-Prot: sp|Q43155|GLTB_SPIOL Ferredoxin-dependent glutamate synthase, chloroplastic; TrEMBL-Plants: tr|A0A0E0QC77|A0A0E0QC77_ORYRU Uncharacterized protein; Found in the gene: LotjaGi1g1v0664300 | 24 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 5.26 |