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IPR011146 is a HIT-like domain.
<p>The histidine triad motif (HIT) consists of the conserved sequence HXHXHXX (where X is a hydrophobic amino acid) at the enzymatic catalytic centre, in which the second histidine is strictly conserved and participates in catalysis with the third histidine [[cite:PUB00097344], [cite:PUB00097347], [cite:PUB00008005]]. Proteins containing HIT domains form a superfamily of nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides [[cite:PUB00008005], [cite:PUB00097344]]. They are highly conserved from archaea to humans and are involved in galactose metabolism, DNA repair, and tumor suppression [[cite:PUB00097349]]. HIT-containing proteins can be divided in five families based on catalytic specificities, sequence compositions, and structural similarities of its members:</p> <ul> <li>Hint family of protein kinase-interacting proteins, the most ancient class in this superfamily. These include adenosine 5'-monophosphoramide hydrolases (e.g. HIT-nucleotide-binding protein, or HINT) [[cite:PUB00097345], [cite:PUB00008005]]. They also have a conserved zinc-binding motif C-X-X-C (where C is a cysteine residue and X is a hydrophobic residue), and a zinc ion is coordinated by these cysteine residues, together with the first histidine residue [[cite:PUB00097349]].</li> <li>Fragile HIT protein, or FINT, whose name is due to its high rate of mutation at its locus on chromosome 3 in many cancers has been characterised as a tumor suppressor and plays a role in the hydrolysis of dinucleotide polyphosphates [[cite:PUB00097347], [cite:PUB00097349]]. HINT and FINT HIT domains have a topology similar to that found in the N-terminal of protein kinases [[cite:PUB00035586]].</li> <li>GalT family. These include specific nucleoside monophosphate transferases (e.g. galactose-1-phosphate uridylyltransferase, diadenosine tetraphosphate phosphorylase, and adenylyl sulphate:phosphate adenylytransferase). These HIT domains are a duplication consisting of 2 HIT-like motifs. This family binds zinc and iron [[cite:PUB00097347], [cite:PUB00097343]].</li> <li>Aprataxin, which hydrolyses both dinucleotide polyphosphates and phophoramidates, and is involved in DNA repair systems [[cite:PUB00097347], [cite:PUB00097349]].</li> <li>mRNA decapping enzyme family. These include enzymes such as DcpS and Dcp2. The HIT-domain is usually C-terminal in these proteins [[cite:PUB00035587], [cite:PUB00097346]].</li> </ul>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Catalytic activity | Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: bis(5'-adenosyl)-triphosphatase-like [Cicer arietinum] gi|502129731|ref|XP_004500406.1| | 9 | ||
| – | PREDICTED: bis(5'-adenosyl)-triphosphatase-like [Cucumis sativus] gi|449495038|ref|XP_004159717.1| | 12 | ||
| – | Histidine triad family protein [Arabidopsis lyrata subsp]. lyrata] gi|297792041|ref|XP_002863905.1| | 11 | ||
| – | PREDICTED: transcription factor bHLH140-like [Glycine max] gi|356496191|ref|XP_003516953.1| | 28 | ||
| – | PREDICTED: 14 kDa zinc-binding protein-like [Cicer arietinum] gi|502091782|ref|XP_004489633.1| | 12 | ||
| – | PREDICTED: histidine triad nucleotide-binding protein 3-like isoform X2 [Cicer arietinum] gi|502160243|ref|XP_004511681.1| | 4 | ||
| – | 14 kDa zinc-binding protein [Medicago truncatula] gi|357475225|ref|XP_003607898.1| | 12 | ||
| – | PREDICTED: zinc finger CCCH domain-containing protein 64-like [Glycine max] gi|356547875|ref|XP_003542330.1| | 16 | ||
| – | PREDICTED: probable galactose-1-phosphate uridyl transferase-like [Glycine max] gi|356559549|ref|XP_003548061.1| | 6 | ||
| – | PREDICTED: probable galactose-1-phosphate uridyl transferase-like [Glycine max] gi|356559549|ref|XP_003548061.1| | 10 | ||
| – | Histidine triad nucleotide-binding protein; TAIR: AT5G48545.1 histidine triad nucleotide-binding 3; Swiss-Prot: sp|F4K1R2|HINT3_ARATH Adenylylsulfatase HINT3; TrEMBL-Plants: tr|A0A072U9P7|A0A072U9P7_MEDTR Histidine triad nucleotide-binding protein; Found in the gene: LotjaGi1g1v0593200 | 17 | ||
| – | Histidine triad nucleotide binding protein; TAIR: AT3G56490.1 HIS triad family protein 3; Swiss-Prot: sp|Q8GUN2|HINT1_ARATH Adenylylsulfatase HINT1; TrEMBL-Plants: tr|I3T4V1|I3T4V1_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0654200 | 13 | ||
| – | Histidine triad nucleotide binding protein; TAIR: AT4G16566.3 histidine triad nucleotide-binding 4; Swiss-Prot: sp|Q84VV6|HINT4_ARATH Bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4; TrEMBL-Plants: tr|A0A1J7GYG8|A0A1J7GYG8_LUPAN Uncharacterized protein; Found in the gene: LotjaGi2g1v0423800 | 10 | ||
| – | Histidine triad nucleotide binding protein; TAIR: AT1G31160.1 HISTIDINE TRIAD NUCLEOTIDE-BINDING 2; Swiss-Prot: sp|P42856|ZB14_MAIZE 14 kDa zinc-binding protein; TrEMBL-Plants: tr|Q2MGQ9|Q2MGQ9_MEDTR Histidine triad (HIT) protein; Found in the gene: LotjaGi4g1v0082900 | 13 | ||
| – | Bis(5'-adenosyl)-triphosphatase; TAIR: AT5G58240.1 FRAGILE HISTIDINE TRIAD; Swiss-Prot: sp|F4KEV7|FHIT_ARATH Bifunctional bis(5'-adenosyl)-triphosphatase/adenylylsulfatase FHIT; TrEMBL-Plants: tr|I3S0X9|I3S0X9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi6g1v0046600 | 14 | ||
| – | Bis(5-adenosyl)-triphosphatase-like protein; TAIR: AT5G58240.1 FRAGILE HISTIDINE TRIAD; Swiss-Prot: sp|F4KEV7|FHIT_ARATH Bifunctional bis(5'-adenosyl)-triphosphatase/adenylylsulfatase FHIT; TrEMBL-Plants: tr|C6T520|C6T520_SOYBN Putative uncharacterized protein; Found in the gene: LotjaGi6g1v0046700 | 15 | ||
| – | Bis(5'-adenosyl)-triphosphatase; TAIR: AT5G58240.1 FRAGILE HISTIDINE TRIAD; Swiss-Prot: sp|F4KEV7|FHIT_ARATH Bifunctional bis(5'-adenosyl)-triphosphatase/adenylylsulfatase FHIT; TrEMBL-Plants: tr|C6T520|C6T520_SOYBN Putative uncharacterized protein; Found in the gene: LotjaGi6g1v0046700 | 12 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| SSF54197 | SUPERFAMILY | 1 | 5.88 |