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IPR011941 is a DNA recombination/repair protein Rad51.
<p>Homologous recombination is an evolutionarily conserved mechanism for the repair of double-strand breaks in DNA and the generation of genetic diversity. The primary function of homologous recombination in mitotic cells is to repair double-strand breaks or single-strand gaps that form as a result of replication fork collapse, from processing of spontaneous damage, and from exposure to DNA-damaging agents. During meiosis, homologous recombination is essential to establish a physical connection between homologous chromosomes to ensure their correct disjunction at the first meiotic division. In addition, the high frequency of meiotic recombination contributes to diversity by creating new linkage arrangements between genes, or parts of genes [[cite:PUB00033348]].</p> <p>The central step of homologous recombination is synapsis, the process of bringing together the two homologous strands. Rad51, a eukaryotic homologue of the prokaryotic recombinase RecA, mediates this process in eukaryotes [[cite:PUB00033349]]. Firstly a single-stranded DNA tail is coated by ATP-bound Rad51 to yield a nucleoprotein filament. This filament then searches for a homologous sequence within double-stranded DNA, and catalyses the exchange of strands between the single-stranded and double-stranded DNA substrates. The original broken end of the resulting branched DNA is now aligned with an appropriate matching sequence in an intact duplex, and is further processed by other enzymes [[cite:PUB00033350]].</p> <p>Rad51 contains an N-terminal α-helical DNA binding domain not found in RecA, and a RecA-like C-terminal ATPase domain [[cite:PUB00014057], [cite:PUB00027524]]. The active form of this protein is a long helical filament where the catalytically active unit is a homodimer [[cite:PUB00031260]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Recombinase activity | Catalysis of the identification and base-pairing of homologous sequences between single-stranded DNA and double-stranded DNA. | ||
| Biological process | Double-strand break repair via homologous recombination | The error-free repair of a double-strand break in DNA in which the broken DNA molecule is repaired using homologous sequences. A strand in the broken DNA searches for a homologous region in an intact chromosome to serve as the template for DNA synthesis. The restoration of two intact DNA molecules results in the exchange, reciprocal or nonreciprocal, of genetic material between the intact DNA molecule and the broken DNA molecule. | ||
| Molecular function | Double-stranded DNA binding | Interacting selectively and non-covalently with double-stranded DNA. | ||
| Molecular function | Single-stranded DNA binding | Interacting selectively and non-covalently with single-stranded DNA. | ||
| Molecular function | DNA-dependent ATPase activity | Catalysis of the reaction: ATP + H2O = ADP + phosphate; this reaction requires the presence of single- or double-stranded DNA, and it drives another reaction. | ||
| Biological process | Mitotic recombination-dependent replication fork processing | Replication fork processing that includes recombination between DNA near the arrested fork and homologous sequences. Proteins involved in homologous recombination are required for replication restart. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: DNA repair protein RAD51 homolog 1-like [Cicer arietinum] gi|502150634|ref|XP_004508049.1| | 24 | ||
| – | DNA repair protein RAD51 like 1; TAIR: AT5G20850.1 RAS associated with diabetes protein 51; Swiss-Prot: sp|P94102|RAD51_ARATH DNA repair protein RAD51 homolog 1; TrEMBL-Plants: tr|A0A151SSM0|A0A151SSM0_CAJCA DNA repair protein RAD51 isogeny 1; Found in the gene: LotjaGi4g1v0319500 | 24 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 50.00 |