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IPR014758 is a Methionyl-tRNA synthetase.
Aminoacyl-tRNA synthetases (also known as aminoacyl-tRNA ligases) catalyse the attachment of amino acids to their cognate transfer RNA molecules through a highly specific two-step reaction [[PMID:10704480], [PMID:12458790]]. These enzymes vary widely in size and oligomeric state, and share limited sequence homology [[PMID:2203971]]. The 20 aminoacyl-tRNA synthetases are divided into two classes. Class I synthetases contain a characteristic Rossmann fold catalytic domain and are predominantly monomeric [[PMID:10673435]]. Class II synthetases feature an antiparallel β-sheet fold flanked by α-helices [[PMID:8364025]] and are mostly dimeric or multimeric, with at least three conserved regions [[PMID:8274143], [PMID:2053131], [PMID:1852601]]. Despite these structural differences, both classes share a conserved α-helical structure involved in tRNA binding. The two classes also differ in their catalytic mechanisms: class I synthetases couple the aminoacyl group to the 2'-hydroxyl of the tRNA, whereas class II synthetases preferentially use the 3'-hydroxyl site. Class I includes synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan, valine, and some lysine synthetases (non-eukaryotic). Class II includes those specific for alanine, asparagine, aspartic acid, glycine, histidine, phenylalanine, proline, serine, threonine, and some lysine synthetases (non-archaeal). Based on their mode of binding to the tRNA acceptor stem, each class has been further subdivided into three subclasses: 1a, 1b, 1c and 2a, 2b, 2c [[PMID:10447505]]. Methionine-tRNA ligase ([ec:6.1.1.10]) is an alpha 2 dimer. In some species (archaea, eubacteria and eukaryotes) a coding sequence, similar to the C-terminal end of MetRS, is present as an independent gene which is a tRNA binding domain as a dimer. In eubacteria, MetRS can also be split in two sub-classes corresponding to the presence of one or two CXXC domains specific to zinc binding. The crystal structures of a number of methionine-tRNA ligases are known [[PMID:10673435], [PMID:2254937], [PMID:10600385]].
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Nucleotide binding | Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose. | ||
| Molecular function | Methionine-tRNA ligase activity | Catalysis of the reaction: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). | ||
| Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
| Biological process | Methionyl-tRNA aminoacylation | The process of coupling methionine to methionyl-tRNA, catalyzed by methionyl-tRNA synthetase. The methionyl-tRNA synthetase is a class-I synthetase. The activated amino acid is transferred to the 2'-OH group of a methionine-accetping tRNA. The 2'-O-aminoacyl-tRNA will ultimately migrate to the 3' position via transesterification. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: probable methionyl-tRNA synthetase-like [Glycine max] gi|356543424|ref|XP_003540160.1| | 30 | ||
| – | Methionine-tRNA ligase, putative; TAIR: AT4G13780.1 methionine-tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS; Swiss-Prot: sp|Q9ZTS1|SYM_ORYSJ Probable methionine--tRNA ligase; TrEMBL-Plants: tr|A0A0B2SPF8|A0A0B2SPF8_GLYSO Putative methionine--tRNA ligase; Found in the gene: LotjaGi3g1v0538600 | 32 | ||
| – | Methionine-tRNA ligase, putative; TAIR: AT4G13780.1 methionine-tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS; Swiss-Prot: sp|Q9ZTS1|SYM_ORYSJ Probable methionine--tRNA ligase; TrEMBL-Plants: tr|A0A0R0H9H8|A0A0R0H9H8_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0538600 | 24 | ||
| – | Methionine-tRNA ligase, putative; TAIR: AT4G13780.1 methionine-tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS; Swiss-Prot: sp|Q9ZTS1|SYM_ORYSJ Probable methionine--tRNA ligase; TrEMBL-Plants: tr|A0A0R0H0Q5|A0A0R0H0Q5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0538600 | 24 | ||
| – | Methionine--tRNA ligase; TAIR: AT3G55400.1 methionyl-tRNA synthetase / methionine-tRNA ligase / MetRS (cpMetRS); Swiss-Prot: sp|Q9M2T9|SYMM_ARATH Methionine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A0S3SL75|A0A0S3SL75_PHAAN Uncharacterized protein; Found in the gene: LotjaGi5g1v0029200 | 27 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd07957 | CDD | 1 | 20.00 |