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IPR015413 is a Methionyl/Leucyl tRNA synthetase.
<p>This entry represents the methionyl and leucyl tRNA synthetases, which are class I aminoacyl-tRNA synthetases.</p> <p>The aminoacyl-tRNA synthetases (also known as aminoacyl-tRNA ligases) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction [[cite:PUB00079872], [cite:PUB00079873]]. These proteins differ widely in size and oligomeric state, and have limited sequence homology [[cite:PUB00007191]]. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric [[cite:PUB00006477]]. Class II aminoacyl-tRNA synthetases share an anti-parallel β-sheet fold flanked by α-helices [[cite:PUB00000386]], and are mostly dimeric or multimeric, containing at least three conserved regions [[cite:PUB00000723], [cite:PUB00005365], [cite:PUB00004391]]. However, tRNA binding involves an α-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan, valine, and some lysine synthetases (non-eukaryotic group) belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, phenylalanine, proline, serine, threonine, and some lysine synthetases (non-archaeal group), belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c [[cite:PUB00007363]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Nucleotide binding | Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose. | ||
| Molecular function | Aminoacyl-tRNA ligase activity | Catalysis of the formation of aminoacyl-tRNA from ATP, amino acid, and tRNA with the release of diphosphate and AMP. | ||
| Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
| Biological process | TRNA aminoacylation for protein translation | The synthesis of aminoacyl tRNA by the formation of an ester bond between the 3'-hydroxyl group of the most 3' adenosine of the tRNA and the alpha carboxylic acid group of an amino acid, to be used in ribosome-mediated polypeptide synthesis. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: methionyl-tRNA synthetase-like [Glycine max] gi|356536480|ref|XP_003536765.1| | 13 | ||
| – | PREDICTED: methionyl-tRNA synthetase-like [Glycine max] gi|356575379|ref|XP_003555819.1| | 10 | ||
| – | PREDICTED: methionyl-tRNA synthetase-like [Glycine max] gi|356575379|ref|XP_003555819.1| | 10 | ||
| – | PREDICTED: probable methionyl-tRNA synthetase-like [Glycine max] gi|356543424|ref|XP_003540160.1| | 30 | ||
| – | PREDICTED: leucyl-tRNA synthetase-like [Glycine max] gi|356522021|ref|XP_003529648.1| | 35 | ||
| – | PREDICTED: leucyl-tRNA synthetase-like [Glycine max] gi|356522021|ref|XP_003529648.1| | 34 | ||
| – | Methionine-tRNA ligase, putative; TAIR: AT4G13780.1 methionine-tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS; Swiss-Prot: sp|Q9ZTS1|SYM_ORYSJ Probable methionine--tRNA ligase; TrEMBL-Plants: tr|A0A0B2SPF8|A0A0B2SPF8_GLYSO Putative methionine--tRNA ligase; Found in the gene: LotjaGi3g1v0538600 | 32 | ||
| – | Methionine-tRNA ligase, putative; TAIR: AT4G13780.1 methionine-tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS; Swiss-Prot: sp|Q9ZTS1|SYM_ORYSJ Probable methionine--tRNA ligase; TrEMBL-Plants: tr|A0A0R0H9H8|A0A0R0H9H8_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0538600 | 24 | ||
| – | Methionine-tRNA ligase, putative; TAIR: AT4G13780.1 methionine-tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS; Swiss-Prot: sp|Q9ZTS1|SYM_ORYSJ Probable methionine--tRNA ligase; TrEMBL-Plants: tr|A0A0R0H0Q5|A0A0R0H0Q5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0538600 | 24 | ||
| – | Leucine--tRNA ligase; TAIR: AT4G04350.1 tRNA synthetase class I (I, L, M and V) family protein; Swiss-Prot: sp|Q9XEA0|SYLM_ARATH Leucine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|G7JJA8|G7JJA8_MEDTR Leucyl-tRNA synthetase; Found in the gene: LotjaGi4g1v0011900 | 35 | ||
| – | Leucine--tRNA ligase; TAIR: AT4G04350.1 tRNA synthetase class I (I, L, M and V) family protein; Swiss-Prot: sp|Q9XEA0|SYLM_ARATH Leucine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|G7JJA8|G7JJA8_MEDTR Leucyl-tRNA synthetase; Found in the gene: LotjaGi4g1v0011900 | 35 | ||
| – | Leucine--tRNA ligase; TAIR: AT4G04350.1 tRNA synthetase class I (I, L, M and V) family protein; Swiss-Prot: sp|Q9XEA0|SYLM_ARATH Leucine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|I1KNG6|I1KNG6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0011900 | 29 | ||
| – | Methionine--tRNA ligase; TAIR: AT3G55400.1 methionyl-tRNA synthetase / methionine-tRNA ligase / MetRS (cpMetRS); Swiss-Prot: sp|Q9M2T9|SYMM_ARATH Methionine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A0S3SL75|A0A0S3SL75_PHAAN Uncharacterized protein; Found in the gene: LotjaGi5g1v0029200 | 27 | ||
| – | Methionine--tRNA ligase; TAIR: AT3G55400.1 methionyl-tRNA synthetase / methionine-tRNA ligase / MetRS (cpMetRS); Swiss-Prot: sp|Q9M2T9|SYMM_ARATH Methionine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A151U822|A0A151U822_CAJCA Methionyl-tRNA synthetase; Found in the gene: LotjaGi5g1v0029200 | 10 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| SSF52374 | SUPERFAMILY | 1 | 7.14 |