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Field | Value |
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Namespace | Cellular component |
Short description | Cdc73/Paf1 complex |
Full defintion | A multiprotein complex that associates with RNA polymerase II and general RNA polymerase II transcription factor complexes and may be involved in both transcriptional initiation and elongation. In Saccharomyces the complex contains Paf1p, Cdc73p, Ctr9p, Rtf1p, and Leo1p. |
Subterm of |
The relationship of GO:0016593 with other GO terms.
Relationship type | GO terms |
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Is a | |
Regulates | n.a. |
Part of | |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0016593, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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PMID | Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex. Mol Cell Biol. 2002 Apr; 22 (7): 1971–80.PMID: 11884586 The Saccharomyces cerevisiae Paf1-RNA polymerase II (Pol II) complex is biochemically and functionally distinct from the Srb-mediator form of Pol II holoenzyme and is required for full expression of a subset of genes. In this work we have used tandem affinity purification tags to isolate the Paf1 complex and mass spectrometry to identify additional components. We have established that Ctr9, Rtf1, and Leo1 are factors that associate with Paf1, Cdc73, and Pol II, but not with the Srb-mediator. Deletion of either PAF1 or CTR9 leads to similar severe pleiotropic phenotypes, which are unaltered when the two mutations are combined. In contrast, we found that deletion of LEO1 or RTF1 leads to few obvious phenotypes, although mutation of RTF1 suppresses mutations in TATA-binding protein, alters transcriptional start sites, and affects elongation. Remarkably, deletion of LEO1 or RTF1 suppresses many paf1Delta phenotypes. In particular, an rtf1Delta paf1Delta double mutant grew faster, was less temperature sensitive, and was more resistant to caffeine and hydroxyurea than a paf1Delta single mutant. In addition, expression of the G(1) cyclin CLN1, reduced nearly threefold in paf1Delta, is restored to wild-type levels in the rtf1Delta paf1Delta double mutant. We suggest that lack of Paf1 results in a defective complex and a block in transcription, which is relieved by removal of Leo1 or Rtf1. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PAF1 complex component [Populus trichocarpa] gi|224121454|ref|XP_002318586.1| | 3 | ||
– | PAF1 complex component [Populus trichocarpa] gi|224121454|ref|XP_002318586.1| | 3 | ||
– | PAF1 complex component [Populus trichocarpa] gi|224121454|ref|XP_002318586.1| | 3 | ||
– | PREDICTED: parafibromin-like [Glycine max] gi|356538299|ref|XP_003537641.1| | 3 | ||
– | PREDICTED: RNA polymerase II-associated factor 1 homolog [Cicer arietinum] gi|502156093|ref|XP_004510311.1| | 3 | ||
– | PREDICTED: RNA polymerase II-associated factor 1 homolog [Cicer arietinum] gi|502156093|ref|XP_004510311.1| | 3 | ||
– | RNA polymerase-associated protein LEO1; TAIR: AT5G61150.2 leo1-like family protein; Swiss-Prot: sp|Q9FNQ0|VIP4_ARATH Protein LEO1 homolog; TrEMBL-Plants: tr|A0A0L9TFD5|A0A0L9TFD5_PHAAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0189700 | 3 | ||
– | RNA polymerase-associated protein LEO1; TAIR: AT5G61150.2 leo1-like family protein; Swiss-Prot: sp|Q9FNQ0|VIP4_ARATH Protein LEO1 homolog; TrEMBL-Plants: tr|A0A0L9TFD5|A0A0L9TFD5_PHAAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0189700 | 3 | ||
– | RNA polymerase-associated protein LEO1; TAIR: AT5G61150.2 leo1-like family protein; Swiss-Prot: sp|Q9FNQ0|VIP4_ARATH Protein LEO1 homolog; TrEMBL-Plants: tr|A0A0L9TFD5|A0A0L9TFD5_PHAAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0189700 | 3 | ||
– | PHP; TAIR: AT3G22590.1 PLANT HOMOLOGOUS TO PARAFIBROMIN; Swiss-Prot: sp|Q9LJ87|CDC73_ARATH Protein CDC73 homolog; TrEMBL-Plants: tr|A0A0L9TBV0|A0A0L9TBV0_PHAAN Uncharacterized protein; Found in the gene: LotjaGi2g1v0452400 | 3 | ||
– | RNA polymerase II-associated factor 1 like; TAIR: AT1G79730.1 hydroxyproline-rich glycoprotein family protein; Swiss-Prot: sp|F4HQA1|PAF1_ARATH Protein PAF1 homolog; TrEMBL-Plants: tr|V7APN8|V7APN8_PHAVU Uncharacterized protein; Found in the gene: LotjaGi3g1v0371200 | 3 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Cellular component | Cdc73/Paf1 complex | 1 | 9.09 |