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IPR001044 is a XPG/Rad2 endonuclease, eukaryotes.
<p>This entry represents XPG (ERCC-5, also known as Rad2 in budding yeast, AtRAD2 or UVH3 in Arabidopsis and Rad13 in fission yeast), a single-stranded structure-specific DNA endonuclease, which cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA [[cite:PUB00062819]]. It makes the 3' incision in DNA nucleotide excision repair (NER); it binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble [[cite:PUB00097845], [cite:PUB00097846]]. XPG is required for DNA replication fork maintenance and preservation of genomic stability [[cite:PUB00097846], [cite:PUB00097847]]. It is involved in homologous recombination repair (HRR) induced by DNA replication stress by recruiting RAD51, BRCA2, and PALB2 to the damaged DNA site [[cite:PUB00097847]]. During HRR, binds to the replication fork with high specificity and stabilizes it [[cite:PUB00097846]] and upstream of HRR, it promotes the release of BRCA1 from DNA [[cite:PUB00097847]].</p> <p>In Saccharomyces cerevisiae, Rad2 forms the Nucleotide Excision Repair Factor 3 (NEF3) complex with a subset of subunits of the transcription factor TFIIH [[cite:PUB00062833]]. Besides DNA damage repair, it is also required for efficient transcription [[cite:PUB00062834]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Single-stranded DNA binding | Interacting selectively and non-covalently with single-stranded DNA. | ||
| Molecular function | Endonuclease activity | Catalysis of the hydrolysis of ester linkages within nucleic acids by creating internal breaks. | ||
| Cellular component | Nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. | ||
| Biological process | Nucleotide-excision repair | A DNA repair process in which a small region of the strand surrounding the damage is removed from the DNA helix as an oligonucleotide. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. Nucleotide excision repair recognizes a wide range of substrates, including damage caused by UV irradiation (pyrimidine dimers and 6-4 photoproducts) and chemicals (intrastrand cross-links and bulky adducts). |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Flap endonuclease 1; TAIR: AT3G28030.1 5'-3' exonuclease family protein; Swiss-Prot: sp|Q9ATY5|UVH3_ARATH DNA repair protein UVH3; TrEMBL-Plants: tr|K7K2I9|K7K2I9_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0605700 | 26 | ||
| – | Flap endonuclease 1; TAIR: AT3G28030.1 5'-3' exonuclease family protein; Swiss-Prot: sp|Q9ATY5|UVH3_ARATH DNA repair protein UVH3; TrEMBL-Plants: tr|K7K2I9|K7K2I9_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0605700 | 24 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 50.00 |