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IPR001154

Source: InterPro

Description

IPR001154 is a DNA topoisomerase II, eukaryotic-type.

<p>This entry represents DNA topoisomerase II enzymes from eukaryotes and viruses. Topoisomerase II primarily functions in introducing negative supercoils into DNA, and is of particular importance during the segregation of chromosomes during mitosis [[cite:PUB00020806], [cite:PUB00004227]]. In eukaryotes and viruses, this enzyme occurs as a single polypeptide, with the N-terminal portion (homologous to subunit B of bacterial topoisomerase II, or gyraseB) responsible for ATPase activity and the C-terminal portion (homologous to subunit A of bacterial topoisomerase II, or gyraseA) responsible for the breaking-rejoining function through its capacity to form protein-DNA bridges. In mammals, there are at least two isozymes of this enzyme, topoisomerases II-alpha and II-beta, which are similar in structure and catalytic properties [[cite:PUB00020807], [cite:PUB00020808]]. The alpha isoform is involved in chromosome condensation and segregation.</p> <p>DNA topoisomerases regulate the number of topological links between two DNA strands (i.e. change the number of superhelical turns) by catalysing transient single-or double-strand breaks, crossing the strands through one another, then resealing the breaks [[cite:PUB00005437]]. These enzymes have several functions: to remove DNA supercoils during transcription and DNA replication; for strand breakage during recombination; for chromosome condensation; and to disentangle intertwined DNA during mitosis [[cite:PUB00020794], [cite:PUB00016842]]. DNA topoisomerases are divided into two classes: type I enzymes ([ec:5.6.2.2]; topoisomerases I, III and V) break single-strand DNA, and type II enzymes ([ec:5.6.2.2]; topoisomerases II, IV and VI) break double-strand DNA [[cite:PUB00020793]].</p> <p>Type II topoisomerases are ATP-dependent enzymes, and can be subdivided according to their structure and reaction mechanisms: type IIA (topoisomerase II or gyrase, and topoisomerase IV) and type IIB (topoisomerase VI). These enzymes are responsible for relaxing supercoiled DNA as well as for introducing both negative and positive supercoils [[cite:PUB00020795]].</p>

This description is obtained from EB-eye REST.

Associated GO terms

GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .

GO term Namespace Name Definition Relationships
GO:0003677
Molecular function DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
Subterm of
GO:0003918
Molecular function DNA topoisomerase type II (ATP-hydrolyzing) activity Catalysis of a DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined; product release is coupled to ATP binding and hydrolysis; changes the linking number in multiples of 2.
Subterm of
GO:0005524
Molecular function ATP binding Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
Subterm of
GO:0006265
Biological process DNA topological change The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number.
Subterm of

Associated Lotus transcripts 5

Transcript Name Description Predicted domains Domain count
Lj3g3v0826110.1
– PREDICTED: DNA topoisomerase 2-like [Glycine max] gi|356555329|ref|XP_003545986.1|
Domains
35
Lj4g3v0877830.1
– PREDICTED: DNA topoisomerase 2-like [Cicer arietinum] gi|502128025|ref|XP_004499912.1|
Domains
20
LotjaGi3g1v0121000.1
– DNA topoisomerase 2; TAIR: AT3G23890.1 topoisomerase II; Swiss-Prot: sp|O24308|TOP2_PEA DNA topoisomerase 2; TrEMBL-Plants: tr|I1MJQ7|I1MJQ7_SOYBN DNA topoisomerase 2; Found in the gene: LotjaGi3g1v0121000
Domains
45
LotjaGi3g1v0121000.2
– DNA topoisomerase 2; TAIR: AT3G23890.2 topoisomerase II; Swiss-Prot: sp|O24308|TOP2_PEA DNA topoisomerase 2; TrEMBL-Plants: tr|I1MJQ7|I1MJQ7_SOYBN DNA topoisomerase 2; Found in the gene: LotjaGi3g1v0121000
Domains
45
LotjaGi4g1v0112600.1
– DNA topoisomerase 2; TAIR: AT3G23890.1 topoisomerase II; Swiss-Prot: sp|O24308|TOP2_PEA DNA topoisomerase 2; TrEMBL-Plants: tr|A0A151TWJ6|A0A151TWJ6_CAJCA DNA topoisomerase 2; Found in the gene: LotjaGi4g1v0112600
Domains
18

Co-occuring domains 1

A list of co-occurring predicted domains within the L. japonicus gene space:

Predicted domain Source Observations Saturation (%)
SSF56719 SUPERFAMILY 1 20.00