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IPR018522 is a DNA topoisomerase, type IIA, conserved site.
<p>DNA topoisomerases regulate the number of topological links between two DNA strands (i.e. change the number of superhelical turns) by catalysing transient single-or double-strand breaks, crossing the strands through one another, then resealing the breaks [[cite:PUB00005437]]. These enzymes have several functions: to remove DNA supercoils during transcription and DNA replication; for strand breakage during recombination; for chromosome condensation; and to disentangle intertwined DNA during mitosis [[cite:PUB00020794], [cite:PUB00016842]]. DNA topoisomerases are divided into two classes: type I enzymes ([ec:5.6.2.2]; topoisomerases I, III and V) break single-strand DNA, and type II enzymes ([ec:5.6.2.2]; topoisomerases II, IV and VI) break double-strand DNA [[cite:PUB00020793]].</p> <p>Type II topoisomerases are ATP-dependent enzymes, and can be subdivided according to their structure and reaction mechanisms: type IIA (topoisomerase II or gyrase, and topoisomerase IV) and type IIB (topoisomerase VI). These enzymes are responsible for relaxing supercoiled DNA as well as for introducing both negative and positive supercoils [[cite:PUB00020795]].</p> <p>DNA topoisomerase II ([ec:5.99.1.3]) [[cite:PUB00001050], [cite:PUB00001088], [cite:PUB00005437]] is one of the two types of enzyme that catalyze the interconversion of topological DNA isomers. Type II topoisomerases are ATP-dependent and act by passing a DNA segment through a transient double-strand break. Topoisomerase II is found in phages, archaebacteria, prokaryotes, eukaryotes, and in African Swine Fever virus (ASF). Bacteriophage T4 topoisomerase II consists of three subunits (the product of genes 39, 52 and 60). In prokaryotes and in archaebacteria the enzyme, known as DNA gyrase, consists of two subunits (genes GyrA and GyrB). In some bacteria, a second type II topoisomerase has been identified; it is known as topoisomerase IV and is required for chromosome segregation, it also consists of two subunits (genes parC and parE). In eukaryotes, type II topoisomerase is a homodimer.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). | ||
| Molecular function | DNA topoisomerase type II (ATP-hydrolyzing) activity | Catalysis of a DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined; product release is coupled to ATP binding and hydrolysis; changes the linking number in multiples of 2. | ||
| Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
| Biological process | DNA topological change | The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: DNA topoisomerase 2-like [Glycine max] gi|356555329|ref|XP_003545986.1| | 35 | ||
| – | PREDICTED: two-component response regulator-like APRR2-like isoform X1 [Cicer arietinum] gi|502152594|ref|XP_004508999.1| | 21 | ||
| – | DNA gyrase subunit B; TAIR: AT5G04130.1 DNA GYRASE B2; Swiss-Prot: sp|Q5YLB4|GYRB_NICBE DNA gyrase subunit B, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A151TTM7|A0A151TTM7_CAJCA DNA gyrase subunit B; Found in the gene: LotjaGi1g1v0663600 | 41 | ||
| – | DNA topoisomerase 2; TAIR: AT3G23890.1 topoisomerase II; Swiss-Prot: sp|O24308|TOP2_PEA DNA topoisomerase 2; TrEMBL-Plants: tr|I1MJQ7|I1MJQ7_SOYBN DNA topoisomerase 2; Found in the gene: LotjaGi3g1v0121000 | 45 | ||
| – | DNA topoisomerase 2; TAIR: AT3G23890.2 topoisomerase II; Swiss-Prot: sp|O24308|TOP2_PEA DNA topoisomerase 2; TrEMBL-Plants: tr|I1MJQ7|I1MJQ7_SOYBN DNA topoisomerase 2; Found in the gene: LotjaGi3g1v0121000 | 45 | ||
| – | Two-component response regulator-like protein; TAIR: AT4G18020.1 CheY-like two-component responsive regulator family protein; Swiss-Prot: sp|Q6LA43|APRR2_ARATH Two-component response regulator-like APRR2; TrEMBL-Plants: tr|A0A072V0B2|A0A072V0B2_MEDTR Two-component response regulator-APRR2-like protein; Found in the gene: LotjaGi4g1v0190400 | 22 | ||
| – | Two-component response regulator-like protein; TAIR: AT4G18020.1 CheY-like two-component responsive regulator family protein; Swiss-Prot: sp|Q6LA43|APRR2_ARATH Two-component response regulator-like APRR2; TrEMBL-Plants: tr|A0A072UR72|A0A072UR72_MEDTR Two-component response regulator-APRR2-like protein; Found in the gene: LotjaGi4g1v0190400 | 22 | ||
| – | Two-component response regulator-like protein; TAIR: AT4G18020.1 CheY-like two-component responsive regulator family protein; Swiss-Prot: sp|Q6LA43|APRR2_ARATH Two-component response regulator-like APRR2; TrEMBL-Plants: tr|A0A072UR72|A0A072UR72_MEDTR Two-component response regulator-APRR2-like protein; Found in the gene: LotjaGi4g1v0190400 | 22 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 12.50 |