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IPR012724 is a Chaperone DnaJ.
<p>Molecular chaperones are a diverse family of proteins that function to protect proteins in the intracellular milieu from irreversible aggregation during synthesis and in times of cellular stress. The bacterial molecular chaperone DnaK is an enzyme that couples cycles of ATP binding, hydrolysis, and ADP release by an N-terminal ATP-hydrolizing domain to cycles of sequestration and release of unfolded proteins by a C-terminal substrate binding domain. Dimeric GrpE is the co-chaperone for DnaK, and acts as a nucleotide exchange factor, stimulating the rate of ADP release 5000-fold [[cite:PUB00005418]]. DnaK is itself a weak ATPase; ATP hydrolysis by DnaK is stimulated by its interaction with another co-chaperone, DnaJ. Thus the co-chaperones DnaJ and GrpE are capable of tightly regulating the nucleotide-bound and substrate-bound state of DnaK in ways that are necessary for the normal housekeeping functions and stress-related functions of the DnaK molecular chaperone cycle.</p> <p>Besides stimulating the ATPase activity of DnaK through its J-domain, DnaJ also associates with unfolded polypeptide chains and prevents their aggregation [[cite:PUB00014967]]. Thus, DnaK and DnaJ may bind to one and the same polypeptide chain to form a ternary complex. The formation of a ternary complex may result in cis-interaction of the J-domain of DnaJ with the ATPase domain of DnaK. An unfolded polypeptide may enter the chaperone cycle by associating first either with ATP-liganded DnaK or with DnaJ. DnaK interacts with both the backbone and side chains of a peptide substrate; it thus shows binding polarity and admits only L-peptide segments. In contrast, DnaJ has been shown to bind both L-and D-peptides and is assumed to interact only with the side chains of the substrate.</p> <p>DnaJ comprises a 70-residue N-terminal domain (the J-domain); a 30-residue glycine-rich region (the G-domain); a central domain containing 4 repeats of a CxxCxGxG motif (the CRR-domain); and a 120-170 residue C-terminal region. The J-and CRR-domains are found in many prokaryotic and eukaryotic proteins [[cite:PUB00005384]], either together or separately.</p> <p>The three components of the DnaK-DnaJ-GrpE system are typically encoded by consecutive genes. DnaJ homologues occur in many genomes, typically not encoded near DnaK and GrpE-like genes. Only some homologues are included in this family.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
GO term | Namespace | Name | Definition | Relationships |
---|---|---|---|---|
Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
Biological process | Protein folding | The process of assisting in the covalent and noncovalent assembly of single chain polypeptides or multisubunit complexes into the correct tertiary structure. | ||
Biological process | Response to heat | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that organism. | ||
Molecular function | Unfolded protein binding | Interacting selectively and non-covalently with an unfolded protein. |
Transcript | Name | Description | Predicted domains | Domain count |
---|---|---|---|---|
– | PREDICTED: chaperone protein DnaJ-like [Glycine max] gi|356522869|ref|XP_003530065.1| | 32 | ||
– | PREDICTED: chaperone protein dnaJ 1, mitochondrial-like isoform X2 [Cicer arietinum] gi|502095201|ref|XP_004490397.1| | 26 | ||
– | PREDICTED: chaperone protein dnaJ 1, mitochondrial-like isoform X3 [Cicer arietinum] gi|502095205|ref|XP_004490398.1| | 26 | ||
– | PREDICTED: chaperone protein DnaJ-like isoform X1 [Cicer arietinum] gi|502080714|ref|XP_004486655.1| | 34 | ||
– | PREDICTED: chaperone protein dnaJ 2-like [Glycine max] gi|356504601|ref|XP_003521084.1| | 24 | ||
– | Chaperone protein DNAj, putative; TAIR: AT1G28210.2 DNAJ heat shock family protein; Swiss-Prot: sp|Q38813|DNAJ1_ARATH Chaperone protein dnaJ 1, mitochondrial; TrEMBL-Plants: tr|K7K9W1|K7K9W1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0140500 | 31 | ||
– | Chaperone protein DNAj, putative; TAIR: AT1G28210.1 DNAJ heat shock family protein; Swiss-Prot: sp|Q38813|DNAJ1_ARATH Chaperone protein dnaJ 1, mitochondrial; TrEMBL-Plants: tr|K7M7R2|K7M7R2_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0140500 | 28 | ||
– | Chaperone protein dnaJ; TAIR: AT2G22360.1 DNAJ heat shock family protein; Swiss-Prot: sp|Q9SJZ7|DNJA6_ARATH Chaperone protein dnaJ A6, chloroplastic; TrEMBL-Plants: tr|I1KT02|I1KT02_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0114200 | 29 | ||
– | Chaperone protein dnaJ; TAIR: AT2G22360.1 DNAJ heat shock family protein; Swiss-Prot: sp|Q9SJZ7|DNJA6_ARATH Chaperone protein dnaJ A6, chloroplastic; TrEMBL-Plants: tr|I1KT02|I1KT02_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0114200 | 29 | ||
– | Chaperone protein dnaJ; TAIR: AT2G22360.1 DNAJ heat shock family protein; Swiss-Prot: sp|Q9SJZ7|DNJA6_ARATH Chaperone protein dnaJ A6, chloroplastic; TrEMBL-Plants: tr|I1KT02|I1KT02_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0114200 | 29 | ||
– | Chaperone protein DnaJ; TAIR: AT3G17830.1 Molecular chaperone Hsp40/DnaJ family protein; Swiss-Prot: sp|Q8DKR7|DNAJ_THEEB Chaperone protein DnaJ; TrEMBL-Plants: tr|I1KJ68|I1KJ68_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0194000 | 30 | ||
– | Chaperone protein DnaJ; TAIR: AT3G17830.1 Molecular chaperone Hsp40/DnaJ family protein; Swiss-Prot: sp|Q8DKR7|DNAJ_THEEB Chaperone protein DnaJ; TrEMBL-Plants: tr|I1KJ68|I1KJ68_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0194000 | 34 | ||
– | Chaperone protein dnaJ; TAIR: AT1G80030.1 Molecular chaperone Hsp40/DnaJ family protein; Swiss-Prot: sp|Q8DKR7|DNAJ_THEEB Chaperone protein DnaJ; TrEMBL-Plants: tr|A0A0B2SH79|A0A0B2SH79_GLYSO Chaperone protein DnaJ; Found in the gene: LotjaGi3g1v0008000 | 36 | ||
– | Chaperone protein DNAj, putative; TAIR: AT5G48030.1 gametophytic factor 2; Swiss-Prot: sp|Q8GWW8|GFA2_ARATH Chaperone protein dnaJ GFA2, mitochondrial; TrEMBL-Plants: tr|A0A151RE69|A0A151RE69_CAJCA Uncharacterized protein; Found in the gene: LotjaGi3g1v0058000 | 29 | ||
– | Chaperone protein dnaJ, putative; TAIR: AT5G22060.1 DNAJ homologue 2; Swiss-Prot: sp|P42825|DNAJ2_ARATH Chaperone protein dnaJ 2; TrEMBL-Plants: tr|I1JMV0|I1JMV0_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0063600 | 29 | ||
– | Chaperone protein dnaJ, putative; TAIR: AT5G22060.1 DNAJ homologue 2; Swiss-Prot: sp|P42825|DNAJ2_ARATH Chaperone protein dnaJ 2; TrEMBL-Plants: tr|I1KJN5|I1KJN5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0063600 | 29 | ||
– | Chaperone protein dnaJ, putative; TAIR: AT5G22060.1 DNAJ homologue 2; Swiss-Prot: sp|P42825|DNAJ2_ARATH Chaperone protein dnaJ 2; TrEMBL-Plants: tr|I1JMV0|I1JMV0_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0063600 | 29 | ||
– | Chaperone protein DNAj, putative; TAIR: AT5G48030.1 gametophytic factor 2; Swiss-Prot: sp|Q8GWW8|GFA2_ARATH Chaperone protein dnaJ GFA2, mitochondrial; TrEMBL-Plants: tr|A0A0L9UJX2|A0A0L9UJX2_PHAAN Uncharacterized protein; Found in the gene: LotjaGi3g1v0124500 | 30 | ||
– | Chaperone protein dnaJ, putative; TAIR: AT5G22060.1 DNAJ homologue 2; Swiss-Prot: sp|P43644|DNJH_ATRNU DnaJ protein homolog ANJ1; TrEMBL-Plants: tr|A0A0D2PQJ2|A0A0D2PQJ2_GOSRA Uncharacterized protein; Found in the gene: LotjaGi3g1v0184100 | 29 | ||
– | Chaperone protein dnaJ, putative; TAIR: AT5G22060.1 DNAJ homologue 2; Swiss-Prot: sp|P43644|DNJH_ATRNU DnaJ protein homolog ANJ1; TrEMBL-Plants: tr|A0A0D2PQJ2|A0A0D2PQJ2_GOSRA Uncharacterized protein; Found in the gene: LotjaGi3g1v0184100 | 29 | ||
– | Chaperone protein dnaJ, putative; TAIR: AT5G22060.1 DNAJ homologue 2; Swiss-Prot: sp|P43644|DNJH_ATRNU DnaJ protein homolog ANJ1; TrEMBL-Plants: tr|A0A0D2VJQ1|A0A0D2VJQ1_GOSRA Uncharacterized protein; Found in the gene: LotjaGi3g1v0184100 | 29 | ||
– | Chaperone protein dnaJ, putative; TAIR: AT5G22060.1 DNAJ homologue 2; Swiss-Prot: sp|Q04960|DNJH_CUCSA DnaJ protein homolog; TrEMBL-Plants: tr|A0A0D2PQJ2|A0A0D2PQJ2_GOSRA Uncharacterized protein; Found in the gene: LotjaGi3g1v0470700 | 30 | ||
– | Chaperone protein dnaJ, putative; TAIR: AT5G22060.1 DNAJ homologue 2; Swiss-Prot: sp|Q04960|DNJH_CUCSA DnaJ protein homolog; TrEMBL-Plants: tr|A0A151R8Y4|A0A151R8Y4_CAJCA DnaJ protein isogeny ANJ1; Found in the gene: LotjaGi3g1v0470700 | 30 | ||
– | Chaperone protein dnaJ, putative; TAIR: AT5G22060.1 DNAJ homologue 2; Swiss-Prot: sp|Q04960|DNJH_CUCSA DnaJ protein homolog; TrEMBL-Plants: tr|A0A151R8Y4|A0A151R8Y4_CAJCA DnaJ protein isogeny ANJ1; Found in the gene: LotjaGi3g1v0470700 | 30 | ||
– | Chaperone protein dnaJ; TAIR: AT2G22360.1 DNAJ heat shock family protein; Swiss-Prot: sp|Q9SJZ7|DNJA6_ARATH Chaperone protein dnaJ A6, chloroplastic; TrEMBL-Plants: tr|A0A151S382|A0A151S382_CAJCA Chaperone protein dnaJ; Found in the gene: LotjaGi6g1v0093400 | 29 |
A list of co-occurring predicted domains within the L. japonicus gene space:
Predicted domain | Source | Observations | Saturation (%) |
---|---|---|---|
cd10747 | CDD | 1 | 4.00 |