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IPR004499 is a Proline-tRNA ligase, class IIa, archaeal-type.
<p>Proline-tRNA ligase (also known as Prolyl-tRNA synthetase) is a class II tRNA ligase and is recognised by [interpro:IPR002314], which recognises tRNA ligases for Gly, His, Ser, and Pro. The proline-tRNA ligases are divided into two widely divergent families. This family includes the archaeal enzyme, the Pro-specific domain of a human multifunctional tRNA ligase, and the enzyme from the spirochete Borrelia burgdorferi (Lyme desease spirochete). The other family, [interpro:IPR004500], includes enzymes from Escherichia coli, Bacillus subtilis, Synechocystis sp. (strain PCC 6308), and one of the two proline-tRNA ligases of Saccharomyces cerevisiae (Baker's yeast).</p> <p>The aminoacyl-tRNA synthetases (also known as aminoacyl-tRNA ligases) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction [[cite:PUB00079872], [cite:PUB00079873]]. These proteins differ widely in size and oligomeric state, and have limited sequence homology [[cite:PUB00007191]]. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric [[cite:PUB00006477]]. Class II aminoacyl-tRNA synthetases share an anti-parallel β-sheet fold flanked by α-helices [[cite:PUB00000386]], and are mostly dimeric or multimeric, containing at least three conserved regions [[cite:PUB00000723], [cite:PUB00005365], [cite:PUB00004391]]. However, tRNA binding involves an α-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan, valine, and some lysine synthetases (non-eukaryotic group) belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, phenylalanine, proline, serine, threonine, and some lysine synthetases (non-archaeal group), belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c [[cite:PUB00007363]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Proline-tRNA ligase activity | Catalysis of the reaction: ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). | ||
| Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
| Cellular component | Cytoplasm | All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. | ||
| Biological process | Prolyl-tRNA aminoacylation | The process of coupling proline to prolyl-tRNA, catalyzed by prolyl-tRNA synthetase. The prolyl-tRNA synthetase is a class-II synthetase. The activated amino acid is transferred to the 3'-OH group of a methionine-accetping tRNA. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: proline--tRNA ligase-like [Cicer arietinum] gi|502132994|ref|XP_004501611.1| | 27 | ||
| – | PREDICTED: bifunctional aminoacyl-tRNA synthetase-like [Glycine max] gi|356572846|ref|XP_003554576.1| | 28 | ||
| – | PREDICTED: bifunctional aminoacyl-tRNA synthetase-like [Glycine max] gi|356572846|ref|XP_003554576.1| | 26 | ||
| – | Proline--tRNA ligase; TAIR: AT3G62120.3 Class II aaRS and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9M1R2|SYPC_ARATH Proline--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1NBI4|I1NBI4_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0754600 | 29 | ||
| – | Proline--tRNA ligase; TAIR: AT3G62120.3 Class II aaRS and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9M1R2|SYPC_ARATH Proline--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1NBI4|I1NBI4_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0754600 | 29 | ||
| – | Proline--tRNA ligase; TAIR: AT5G52520.1 Class II aaRS and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9FYR6|SYPM_ARATH Proline--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|V7C4B0|V7C4B0_PHAVU Uncharacterized protein; Found in the gene: LotjaGi2g1v0122700 | 33 | ||
| – | Proline--tRNA ligase; TAIR: AT3G62120.3 Class II aaRS and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9M1R2|SYPC_ARATH Proline--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1NBI4|I1NBI4_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0330900 | 27 | ||
| – | Proline--tRNA ligase; TAIR: AT3G62120.3 Class II aaRS and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9M1R2|SYPC_ARATH Proline--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1NBI4|I1NBI4_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0009800 | 29 | ||
| – | Proline--tRNA ligase; TAIR: AT3G62120.3 Class II aaRS and biotin synthetases superfamily protein; Swiss-Prot: sp|Q9M1R2|SYPC_ARATH Proline--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|I1NBI4|I1NBI4_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0009800 | 29 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 11.11 |