Your browser is unable to support new features implemented in HTML5 and CSS3 to render this site as intended. Your experience may suffer from functionality degradation but the site should remain usable. We strongly recommend the latest version of Google Chrome, OS X Safari or Mozilla Firefox. As Safari is bundled with OS X, if you are unable to upgrade to a newer version of OS X, we recommend using an open source browser. Dismiss message
IPR005839 is a Methylthiotransferase.
<p>The methylthiotransferase (MTTase) or miaB-like family is named after the (dimethylallyl)adenosine tRNA MTTase miaB protein, which catalyses a C-H to C-S bond conversion in the methylthiolation of tRNA. A related bacterial enzyme RimO performs a similar methylthiolation, but on a protein substrate. RimO acts on the ribosomal protein S12 and forms a separate MTTase subfamily. The miaB-subfamily includes mammalian CDK5 regulatory subunit-associated proteins and similar proteins in other eukaryotes. Two other subfamilies, yqeV and CDKAL1, are named after a Bacillus subtilis and a human protein, respectively. While yqeV-like proteins are found in bacteria, CDKAL1 subfamily members occur in eukaryotes and in archaebacteria [[cite:PUB00083160]].</p> <p>The likely MTTases from these 4 subfamilies contain an N-terminal MTTase domain, a central radical generating fold and a C-terminal TRAM domain (see [interpro:IPR002792]). The core forms a radical SAM fold (or AdoMet radical), containing a cysteine motif CxxxCxxC that binds a [4Fe-4S] cluster [[cite:PUB00009728], [cite:PUB00046148], [cite:PUB00010539]]. A reducing equivalent from the [4Fe-4S]+ cluster is used to cleave S-adenosylmethionine (SAM) to generate methionine and a 5'-deoxyadenosyl radical. The latter is thought to produce a reactive substrate radical that is amenable to sulphur insertion [[cite:PUB00046148], [cite:PUB00010539]]. The N-terminal MTTase domain contains 3 cysteines that bind a second [4Fe-4S] cluster, in addition to the radical-generating [4Fe-4S] cluster, which could be involved in the thiolation reaction. The C-terminal TRAM domain is not shared with other radical SAM proteins outside the MTTase family. The TRAM domain can bind to RNA substrate and seems to be important for substrate recognition. The tertiary structure of the central radical SAM fold has six β/α motifs resembling a three-quarter TIM barrel core [[cite:PUB00052321]]. The N-terminal MTTase domain might form an additional [β/α]2 TIM barrel unit [[cite:PUB00046148]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | TRNA modification | The covalent alteration of one or more nucleotides within a tRNA molecule to produce a tRNA molecule with a sequence that differs from that coded genetically. | ||
| Molecular function | Transferase activity | Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2. | ||
| Molecular function | 4 iron, 4 sulfur cluster binding | Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: CDK5RAP1-like protein-like [Glycine max] gi|356540793|ref|XP_003538869.1| | 33 | ||
| – | PREDICTED: CDKAL1-like protein-like [Glycine max] gi|356542899|ref|XP_003539902.1| | 29 | ||
| – | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT4G36390.1 Methylthiotransferase; Swiss-Prot: sp|Q8H0V1|CK5P1_ARATH CDK5RAP1-like protein; TrEMBL-Plants: tr|G7KGB3|G7KGB3_MEDTR Radical SAM methylthiotransferase, MiaB/RimO family protein; Found in the gene: LotjaGi2g1v0385400 | 28 | ||
| – | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT4G36390.1 Methylthiotransferase; Swiss-Prot: sp|Q2R1U4|CK5P1_ORYSJ CDK5RAP1-like protein; TrEMBL-Plants: tr|K7LNH5|K7LNH5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0385400 | 26 | ||
| – | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT4G36390.1 Methylthiotransferase; Swiss-Prot: sp|Q2R1U4|CK5P1_ORYSJ CDK5RAP1-like protein; TrEMBL-Plants: tr|K7LNH5|K7LNH5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0385400 | 26 | ||
| – | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT4G36390.1 Methylthiotransferase; Swiss-Prot: sp|Q2R1U4|CK5P1_ORYSJ CDK5RAP1-like protein; TrEMBL-Plants: tr|K7LNH5|K7LNH5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0385400 | 26 | ||
| – | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT1G72090.1 Methylthiotransferase; Swiss-Prot: sp|Q6P4Y0|CDKAL_XENTR Threonylcarbamoyladenosine tRNA methylthiotransferase; TrEMBL-Plants: tr|I1LNN6|I1LNN6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0556600 | 29 | ||
| – | TRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; TAIR: AT1G72090.1 Methylthiotransferase; Swiss-Prot: sp|Q6NS26|CDKAL_XENLA Threonylcarbamoyladenosine tRNA methylthiotransferase; TrEMBL-Plants: tr|I1LNN6|I1LNN6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0556600 | 29 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd01335 | CDD | 1 | 12.50 |