Your browser is unable to support new features implemented in HTML5 and CSS3 to render this site as intended. Your experience may suffer from functionality degradation but the site should remain usable. We strongly recommend the latest version of Google Chrome, OS X Safari or Mozilla Firefox. As Safari is bundled with OS X, if you are unable to upgrade to a newer version of OS X, we recommend using an open source browser. Dismiss message
IPR001818 is a Peptidase M10, metallopeptidase.
<p>Over 70 metallopeptidase families have been identified to date. In these enzymes a divalent cation which is usually zinc, but may be cobalt, manganese or copper, activates the water molecule. The metal ion is held in place by amino acid ligands, usually three in number. In some families of co-catalytic metallopeptidases, two metal ions are observed in crystal structures ligated by five amino acids, with one amino acid ligating both metal ions. The known metal ligands are His, Glu, Asp or Lys. At least one other residue is required for catalysis, which may play an electrophillic role. Many metalloproteases contain an HEXXH motif, which has been shown in crystallographic studies to form part of the metal-binding site [[cite:PUB00003579]]. The HEXXH motif is relatively common, but can be more stringently defined for metalloproteases as 'abXHEbbHbc', where 'a' is most often valine or threonine and forms part of the S1' subsite in thermolysin and neprilysin, 'b' is an uncharged residue, and 'c' a hydrophobic residue. Proline is never found in this site, possibly because it would break the helical structure adopted by this motif in metalloproteases [[cite:PUB00003579]].</p> <p>This group of metallopeptidases belong to the MEROPS peptidase family M10 (clan MA(M)).</p> <p>The protein fold of the peptidase domain for members of this family resembles that of thermolysin, the type example for clan MA.</p> <p>Sequences having this domain are extracellular metalloproteases, such as collagenase and stromelysin, which degrade the extracellular matrix, are known as matrixins. They are zinc-dependent, calcium-activated proteases synthesised as inactive precursors (zymogens), which are proteolytically cleaved to yield the active enzyme [[cite:PUB00002514], [cite:PUB00001191]]. All matrixins and related proteins possess 2 domains: an N-terminal domain, and a zinc-binding active site domain. The N-terminal domain peptide, cleaved during the activation step, includes a conserved PRCGVPDV octapeptide, known as the cysteine switch, whose Cys residue chelates the active site zinc atom, rendering the enzyme inactive [[cite:PUB00002456], [cite:PUB00002651]]. The active enzyme degrades components of the extracellular matrix, playing a role in the initial steps of tissue remodelling during morphogenesis, wound healing, angiogenesis and tumour invasion [[cite:PUB00002514], [cite:PUB00001191]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
GO term | Namespace | Name | Definition | Relationships |
---|---|---|---|---|
Molecular function | Metalloendopeptidase activity | Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions. | ||
Biological process | Proteolysis | The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds. | ||
Molecular function | Zinc ion binding | Interacting selectively and non-covalently with zinc (Zn) ions. | ||
Cellular component | Extracellular matrix | A structure lying external to one or more cells, which provides structural support, biochemical or biomechanical cues for cells or tissues. |
Transcript | Name | Description | Predicted domains | Domain count |
---|---|---|---|---|
– | Matrix metalloprotease [Medicago truncatula] gi|357486209|ref|XP_003613392.1| | 21 | ||
– | Matrix metalloprotease [Medicago truncatula] gi|357486209|ref|XP_003613392.1| | 23 | ||
– | PREDICTED: metalloendoproteinase 1-like [Cicer arietinum] gi|502141260|ref|XP_004504503.1| | 25 | ||
– | Matrix metalloproteinase-28 [Medicago truncatula] gi|357482059|ref|XP_003611315.1| | 13 | ||
– | Matrix metalloprotease [Medicago truncatula] gi|357486209|ref|XP_003613392.1| | 15 | ||
– | Matrix metalloproteinase-27 [Medicago truncatula] gi|357512801|ref|XP_003626689.1| | 25 | ||
– | PREDICTED: metalloendoproteinase 1-like [Glycine max] gi|356501971|ref|XP_003519796.1| | 10 | ||
– | Matrix metalloprotease [Medicago truncatula] gi|357486209|ref|XP_003613392.1| | 25 | ||
– | PREDICTED: metalloendoproteinase 1-like [Glycine max] gi|356540528|ref|XP_003538740.1| | 24 | ||
– | PREDICTED: metalloendoproteinase 1-like [Cicer arietinum] gi|502154901|ref|XP_004509890.1| | 25 | ||
– | Metalloendoproteinase 1; TAIR: AT1G70170.1 matrix metalloproteinase; Swiss-Prot: sp|O04529|2MMP_ARATH Metalloendoproteinase 2-MMP; TrEMBL-Plants: tr|A0A151TFV0|A0A151TFV0_CAJCA Metalloendoproteinase 1; Found in the gene: LotjaGi2g1v0313400 | 27 | ||
– | Matrix metalloproteinase; TAIR: AT1G70170.1 matrix metalloproteinase; Swiss-Prot: sp|O04529|2MMP_ARATH Metalloendoproteinase 2-MMP; TrEMBL-Plants: tr|V7CH34|V7CH34_PHAVU Uncharacterized protein; Found in the gene: LotjaGi2g1v0313500_LC | 9 | ||
– | Matrix metalloproteinase; TAIR: AT1G59970.1 Matrixin family protein; Swiss-Prot: sp|O04529|2MMP_ARATH Metalloendoproteinase 2-MMP; TrEMBL-Plants: tr|G7K5I7|G7K5I7_MEDTR Matrix metalloproteinase; Found in the gene: LotjaGi2g1v0313600 | 22 | ||
– | Metalloendoproteinase 1; TAIR: AT4G16640.1 Matrixin family protein; Swiss-Prot: sp|O23507|1MMP_ARATH Metalloendoproteinase 1-MMP; TrEMBL-Plants: tr|I1LGQ7|I1LGQ7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0422500 | 25 | ||
– | Metalloendoproteinase 1; TAIR: AT2G45040.1 Matrixin family protein; Swiss-Prot: sp|Q8GWW6|4MMP_ARATH Metalloendoproteinase 4-MMP; TrEMBL-Plants: tr|A0A151RA26|A0A151RA26_CAJCA Metalloendoproteinase 1; Found in the gene: LotjaGi3g1v0050100 | 26 | ||
– | Matrix metalloproteinase; TAIR: AT1G70170.1 matrix metalloproteinase; Swiss-Prot: sp|O04529|2MMP_ARATH Metalloendoproteinase 2-MMP; TrEMBL-Plants: tr|V7C6E0|V7C6E0_PHAVU Uncharacterized protein; Found in the gene: LotjaGi3g1v0142200 | 26 |
A list of co-occurring predicted domains within the L. japonicus gene space:
Predicted domain | Source | Observations | Saturation (%) |
---|---|---|---|
cd04278 | CDD | 1 | 6.25 |