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IPR007685

Source: InterPro

Description

IPR007685 is a RelA/SpoT.

<p>This entry includes the catalytic domains of Escherichia coli ppGpp synthetase (RelA), ppGpp synthetase/hydrolase (SpoT), and related proteins. The functions of Escherichia coli RelA and SpoT differ somewhat. RelA ([ec:2.7.6.5]) produces pppGpp (or ppGpp) from ATP and GTP (or GDP) in response to amino-acid starvation and in association with ribosomes [[cite:PUB00081128]]. SpoT ([ec:3.1.7.2]) degrades ppGpp, but may also act as a secondary ppGpp synthetase under carbon limitation in a ribosome-independent manner [[cite:PUB00081126], [cite:PUB00081127]]. Gram-negative bacteria have two enzymes involved in (p)ppGpp metabolism while most Gram-positive organisms have a single Rel-Spo enzyme (Rel), which both synthesizes and degrades (p)ppGpp [[cite:PUB00081129], [cite:PUB00081130]]. The Arabidopsis thaliana Rel-Spo proteins, At-RSH1, At-RSH2, and At-RSH3 appear to regulate a rapid (p)ppGpp-mediated response to pathogens and other stresses [[cite:PUB00079747]]. This catalytic domain is found in association with an N-terminal HD domain and a C-terminal metal dependent phosphohydrolase domain (TGS). Some Rel-Spo proteins also have a C-terminal regulatory ACT domain. This subgroup belongs to the Pol beta-like NT superfamily [[cite:PUB00013566], [cite:PUB00005745]].</p> <p>In the Pol beta-like NT superfamily [[cite:PUB00013566], [cite:PUB00005745]], the majority of enzymes have two carboxylates, Dx[D/E], together with a third more distal carboxylate, which coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family.</p>

This description is obtained from EB-eye REST.

Associated GO terms

GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .

GO term Namespace Name Definition Relationships
GO:0015969
Biological process Guanosine tetraphosphate metabolic process The chemical reactions and pathways involving guanine tetraphosphate (5'-ppGpp-3'), a derivative of guanine riboside with four phosphates.
Subterm of

Associated Lotus transcripts 22

Transcript Name Description Predicted domains Domain count
Lj1g3v3717020.1
– PREDICTED: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase-like isoform X1 [Cicer arietinum] gi|502159240|ref|XP_004511439.1|
Domains
13
Lj1g3v4875630.1
– PREDICTED: GTP pyrophosphokinase-like isoform X1 [Cicer arietinum] gi|502112635|ref|XP_004494394.1|
Domains
25
Lj2g3v2124850.1
– PREDICTED: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase-like isoform X1 [Cicer arietinum] gi|502159240|ref|XP_004511439.1|
Domains
17
Lj2g3v2124850.2
– PREDICTED: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase-like isoform X2 [Cicer arietinum] gi|502159243|ref|XP_004511440.1|
Domains
8
Lj3g3v2577230.1
– RelA-SpoT like protein PsRSH1 [Pisum sativum] gi|37196706|dbj|BAC97801.1|
Domains
15
Lj4g3v2249040.1
– PREDICTED: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase-like [Glycine max] gi|356548029|ref|XP_003542406.1|
Domains
21
Lj4g3v2249040.2
– GTP pyrophosphokinase [Medicago truncatula] gi|357479179|ref|XP_003609875.1|
Domains
21
LotjaGi1g1v0763000.1
– GTP pyrophosphokinase; TAIR: AT4G02260.2 RELA/SPOT homolog 1; Swiss-Prot: sp|P0DKG8|RSH1L_ARATH Putative GTP diphosphokinase RSH1, chloroplastic; TrEMBL-Plants: tr|A0A0B2QGP0|A0A0B2QGP0_GLYSO GTP pyrophosphokinase; Found in the gene: LotjaGi1g1v0763000
Domains
31
LotjaGi1g1v0763000.2
– GTP pyrophosphokinase; TAIR: AT4G02260.2 RELA/SPOT homolog 1; Swiss-Prot: sp|P0DKG8|RSH1L_ARATH Putative GTP diphosphokinase RSH1, chloroplastic; TrEMBL-Plants: tr|A0A0B2QGP0|A0A0B2QGP0_GLYSO GTP pyrophosphokinase; Found in the gene: LotjaGi1g1v0763000
Domains
32
LotjaGi1g1v0763000.3
– GTP pyrophosphokinase; TAIR: AT4G02260.2 RELA/SPOT homolog 1; Swiss-Prot: sp|P0DKG8|RSH1L_ARATH Putative GTP diphosphokinase RSH1, chloroplastic; TrEMBL-Plants: tr|A0A0B2QGP0|A0A0B2QGP0_GLYSO GTP pyrophosphokinase; Found in the gene: LotjaGi1g1v0763000
Domains
32
LotjaGi1g1v0763000.4
– GTP pyrophosphokinase; TAIR: AT4G02260.2 RELA/SPOT homolog 1; Swiss-Prot: sp|P0DKG8|RSH1L_ARATH Putative GTP diphosphokinase RSH1, chloroplastic; TrEMBL-Plants: tr|A0A0B2QGP0|A0A0B2QGP0_GLYSO GTP pyrophosphokinase; Found in the gene: LotjaGi1g1v0763000
Domains
31
LotjaGi2g1v0448600.1
– Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase; TAIR: AT1G54130.1 RELA/SPOT homolog 3; Swiss-Prot: sp|Q9SYH1|RSH3C_ARATH Probable GTP diphosphokinase RSH3, chloroplastic; TrEMBL-Plants: tr|A0A0B2R105|A0A0B2R105_GLYSO Putative GTP pyrophosphokinase; Found in the gene: LotjaGi2g1v0448600
Domains
22
LotjaGi2g1v0448600.2
– Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase; TAIR: AT1G54130.1 RELA/SPOT homolog 3; Swiss-Prot: sp|Q9SYH1|RSH3C_ARATH Probable GTP diphosphokinase RSH3, chloroplastic; TrEMBL-Plants: tr|A0A0B2R105|A0A0B2R105_GLYSO Putative GTP pyrophosphokinase; Found in the gene: LotjaGi2g1v0448600
Domains
22
LotjaGi2g1v0448600.3
– Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase, putative; TAIR: AT1G54130.1 RELA/SPOT homolog 3; Swiss-Prot: sp|Q9SYH1|RSH3C_ARATH Probable GTP diphosphokinase RSH3, chloroplastic; TrEMBL-Plants: tr|A0A0R0HH22|A0A0R0HH22_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0448600
Domains
22
LotjaGi2g1v0448600.4
– Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase; TAIR: AT1G54130.1 RELA/SPOT homolog 3; Swiss-Prot: sp|Q9SYH1|RSH3C_ARATH Probable GTP diphosphokinase RSH3, chloroplastic; TrEMBL-Plants: tr|A0A0R0LJ13|A0A0R0LJ13_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0448600
Domains
22
LotjaGi2g1v0448600.5
– Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase; TAIR: AT1G54130.1 RELA/SPOT homolog 3; Swiss-Prot: sp|Q9SYH1|RSH3C_ARATH Probable GTP diphosphokinase RSH3, chloroplastic; TrEMBL-Plants: tr|A0A0R0LJ13|A0A0R0LJ13_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0448600
Domains
22
LotjaGi2g1v0448600.6
– Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase; TAIR: AT1G54130.1 RELA/SPOT homolog 3; Swiss-Prot: sp|Q9SYH1|RSH3C_ARATH Probable GTP diphosphokinase RSH3, chloroplastic; TrEMBL-Plants: tr|A0A0B2R105|A0A0B2R105_GLYSO Putative GTP pyrophosphokinase; Found in the gene: LotjaGi2g1v0448600
Domains
22
LotjaGi2g1v0448600.7
– Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase; TAIR: AT1G54130.1 RELA/SPOT homolog 3; Swiss-Prot: sp|Q9SYH1|RSH3C_ARATH Probable GTP diphosphokinase RSH3, chloroplastic; TrEMBL-Plants: tr|A0A0B2R105|A0A0B2R105_GLYSO Putative GTP pyrophosphokinase; Found in the gene: LotjaGi2g1v0448600
Domains
16
LotjaGi2g1v0448600.8
– Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase; TAIR: AT1G54130.1 RELA/SPOT homolog 3; Swiss-Prot: sp|Q9SYH1|RSH3C_ARATH Probable GTP diphosphokinase RSH3, chloroplastic; TrEMBL-Plants: tr|A0A0B2R105|A0A0B2R105_GLYSO Putative GTP pyrophosphokinase; Found in the gene: LotjaGi2g1v0448600
Domains
16
LotjaGi3g1v0414100.1
– Rela/spot homolog 3 family protein; TAIR: AT1G54130.1 RELA/SPOT homolog 3; Swiss-Prot: sp|Q9SYH1|RSH3C_ARATH Probable GTP diphosphokinase RSH3, chloroplastic; TrEMBL-Plants: tr|A0A151S6W9|A0A151S6W9_CAJCA Putative guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase; Found in the gene: LotjaGi3g1v0414100
Domains
17
LotjaGi3g1v0414100.2
– RELA/SPOT; TAIR: AT1G54130.1 RELA/SPOT homolog 3; Swiss-Prot: sp|Q9M5P5|RSH3L_ARATH Probable GTP diphosphokinase RSH3, chloroplastic; TrEMBL-Plants: tr|A0A151S6W9|A0A151S6W9_CAJCA Putative guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase; Found in the gene: LotjaGi3g1v0414100
Domains
12
LotjaGi4g1v0313700.1
– RELA/SPOT homolog 3; TAIR: AT3G17470.2 Ca2+-activated RelA/spot-like protein; Swiss-Prot: sp|Q84R11|CRSH_ARATH Probable GTP diphosphokinase CRSH, chloroplastic; TrEMBL-Plants: tr|G7JR05|G7JR05_MEDTR Ca2+-activated RelA/SpoT-like protein; Found in the gene: LotjaGi4g1v0313700
Domains
24

Co-occuring domains 1

A list of co-occurring predicted domains within the L. japonicus gene space:

Predicted domain Source Observations Saturation (%)
cd05399 CDD 1 4.55