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IPR036599 is a DNA ligase, ATP-dependent, N-terminal domain superfamily.
<p>DNA ligase (polydeoxyribonucleotide synthase) is the enzyme that joins two DNA fragments by catalysing the formation of an internucleotide ester bond between phosphate and deoxyribose. It is active during DNA replication, DNA repair and DNA recombination. There are two forms of DNA ligase, one requires ATP ([ec:6.5.1.1]), the other NAD ([ec:6.5.1.2]), the latter being restricted to eubacteria. Eukaryotic, archaebacterial, viral and some eubacterial DNA ligases are ATP-dependent. The first step in the ligation reaction is the formation of a covalent enzyme-AMP complex. The co-factor ATP is cleaved to pyrophosphate and AMP, with the AMP being covalently joined to a highly conserved lysine residue in the active site of the ligase. The activated AMP residue is then transferred to the 5'phosphate of the nick, before the nick is sealed by phosphodiester-bond formation and AMP elimination [[cite:PUB00004738], [cite:PUB00000083]].</p> <p>This domain superfamily is found in many but not all ATP-dependent DNA ligase enzymes ([ec:6.5.1.1]). It is thought to be involved in DNA binding and in catalysis. In human DNA ligase I ([swissprot:P18858]), and in Saccharomyces cerevisiae (Baker's yeast) ([swissprot:P04819]), this region was necessary for catalysis, and separated from the amino terminus by targeting elements. In Vaccinia virus ([swissprot:P16272]) this region was not essential for catalysis, but deletion decreases the affinity for nicked DNA and decreased the rate of strand joining at a step subsequent to enzyme-adenylate formation [[cite:PUB00016293]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). | ||
| Molecular function | DNA ligase (ATP) activity | Catalysis of the reaction: ATP + deoxyribonucleotide(n) + deoxyribonucleotide(m) = AMP + diphosphate + deoxyribonucleotide(n+m). | ||
| Biological process | DNA repair | The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway. | ||
| Biological process | DNA recombination | Any process in which a new genotype is formed by reassortment of genes resulting in gene combinations different from those that were present in the parents. In eukaryotes genetic recombination can occur by chromosome assortment, intrachromosomal recombination, or nonreciprocal interchromosomal recombination. Interchromosomal recombination occurs by crossing over. In bacteria it may occur by genetic transformation, conjugation, transduction, or F-duction. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | DNA ligase; TAIR: AT1G66730.1 DNA LIGASE 6; Swiss-Prot: sp|F4HPZ9|LIG6_ARATH DNA ligase 6; TrEMBL-Plants: tr|G7KV95|G7KV95_MEDTR DNA ligase; Found in the gene: LotjaGi1g1v0545100 | 31 | ||
| – | DNA ligase; TAIR: AT1G66730.1 DNA LIGASE 6; Swiss-Prot: sp|F4HPZ9|LIG6_ARATH DNA ligase 6; TrEMBL-Plants: tr|A0A0L9TDF0|A0A0L9TDF0_PHAAN DNA ligase; Found in the gene: LotjaGi1g1v0545100 | 34 | ||
| – | DNA ligase; TAIR: AT1G08130.1 DNA ligase 1; Swiss-Prot: sp|Q42572|DNLI1_ARATH DNA ligase 1; TrEMBL-Plants: tr|A0A072UVT2|A0A072UVT2_MEDTR DNA ligase; Found in the gene: LotjaGi3g1v0485100 | 27 | ||
| – | DNA ligase; TAIR: AT5G57160.1 DNA ligase IV; Swiss-Prot: sp|Q9LL84|DNLI4_ARATH DNA ligase 4; TrEMBL-Plants: tr|K7MBU8|K7MBU8_SOYBN DNA ligase; Found in the gene: LotjaGi6g1v0235200 | 36 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 25.00 |