Your browser is unable to support new features implemented in HTML5 and CSS3 to render this site as intended. Your experience may suffer from functionality degradation but the site should remain usable. We strongly recommend the latest version of Google Chrome, OS X Safari or Mozilla Firefox. As Safari is bundled with OS X, if you are unable to upgrade to a newer version of OS X, we recommend using an open source browser. Dismiss message
IPR002421 is a 5'-3' exonuclease.
<p>This entry represents flap endonucleases from eukaryotes, bacteria, viruses and archaea. Flap endonucleases (FENs) catalyse the exonucleolytic hydrolysis of blunt-ended duplex DNA substrates and the endonucleolytic cleavage of 5'-bifurcated nucleic acids at the junction formed between single and double-stranded DNA [[cite:PUB00094488]].</p> <p>In prokaryotes, the essential FEN reaction can be performed by the N-terminal 5'-3' exonuclease domain present on DNA polymerase I. Some eubacteria, however, possess a second gene encoding a 5'-3' exonuclease domain [[cite:PUB00069563], [cite:PUB00069558]]. Two distinct classes of these independent bacterial FENs exist: Xni (ExoIXI) from Escherichia coli and SaFEN (Staphylococcus aureus FEN). SaFEN has both FEN and 5'-3' exonuclease activities. Xni (ExoIX) was previously identified as a 3'-5' exonuclease and named exonuclease IX (exonuclease 9) [[cite:PUB00053938], [cite:PUB00053939]] but subsequently found to possess flap endonuclease activity, but not exonuclease activity [[cite:PUB00053941], [cite:PUB00069563], [cite:PUB00069558]].</p> <p>Archaea, eukaryotes, bacteriophages and some viruses encode a separate FEN enzyme but lack FEN domains on their DNA polymerases [[cite:PUB00069558]]. Escherichia phage T5 encodes the flap endonuclease D15, which catalyzes both the 5'-exonucleolytic and structure-specific endonucleolytic hydrolysis of DNA branched nucleic acid molecules [[cite:PUB00011714], [cite:PUB00031897], [cite:PUB00094486]]. In bacteriophage T4, disruption of the rnh gene (which encodes a FEN, known historically as T4 RNase H) results in slower, less accurate DNA replication. Bacteriophage T4 has both 5' nuclease and flap endonuclease activities [[cite:PUB00047705]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: DNA polymerase I-like isoform X2 [Cicer arietinum] gi|502142965|ref|XP_004505167.1| | 11 | ||
| – | 5'-3' exonuclease; TAIR: AT1G34380.2 5'-3' exonuclease family protein; Swiss-Prot: sp|O67550|EX53_AQUAE 5'-3' exonuclease; TrEMBL-Plants: tr|K7MHK5|K7MHK5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0098200 | 16 | ||
| – | Flap endonuclease Xni; TAIR: AT3G52050.1 5'-3' exonuclease family protein; Swiss-Prot: sp|O52225|DPO1_THEFI DNA polymerase I, thermostable; TrEMBL-Plants: tr|A0A0S3R340|A0A0S3R340_PHAAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0069300 | 19 | ||
| – | Flap endonuclease 1; TAIR: AT5G26680.2 5'-3' exonuclease family protein; Swiss-Prot: sp|C6TEX6|FEN1_SOYBN Flap endonuclease 1; TrEMBL-Plants: tr|I3SAA1|I3SAA1_LOTJA Flap endonuclease 1; Found in the gene: LotjaGi5g1v0234800 | 27 | ||
| – | Flap endonuclease 1; TAIR: AT5G26680.3 5'-3' exonuclease family protein; Swiss-Prot: sp|C6TEX6|FEN1_SOYBN Flap endonuclease 1; TrEMBL-Plants: tr|A0A072VM97|A0A072VM97_MEDTR Flap endonuclease 1; Found in the gene: LotjaGi5g1v0234800 | 26 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd09907 | CDD | 1 | 20.00 |