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Field | Value |
---|---|
Namespace | Molecular function |
Short description | Chitinase activity |
Full defintion | Catalysis of the hydrolysis of (1->4)-beta linkages of N-acetyl-D-glucosamine (GlcNAc) polymers of chitin and chitodextrins. |
Subterm of |
The relationship of GO:0004568 with other GO terms.
Relationship type | GO terms |
---|---|
Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0004568, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
---|---|
EC | 3.2.1.14 |
GOC | pde |
PMID | Different cleavage specificities of the dual catalytic domains in chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J Biol Chem. 2001 Sep 21; 276 (38): 35629–35.PMID: 11468293 The chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1, Tk-ChiA, has an interesting multidomain structure containing dual catalytic domains and triple chitin-binding domains. To determine the biochemical properties of each domain, we constructed deletion mutant genes corresponding to the individual catalytic domains and purified the recombinant proteins. A synergistic effect was observed when chitin was degraded in the presence of both catalytic domains, suggesting different cleavage specificity of these domains. Analyses of degradation products from N-acetyl-chitooligosaccharides and their chromogenic derivatives with thin layer chromatography indicated that the N-terminal catalytic domain mainly hydrolyzed the second glycosidic bond from the nonreducing end of the oligomers, whereas the C-terminal domain randomly hydrolyzed glycosidic bonds other than the first bond from the nonreducing end. Both catalytic domains formed diacetyl-chitobiose as a major end product and possessed transglycosylation activity. Further analysis of degradation products from colloidal chitin with high performance liquid chromatography showed that the N-terminal catalytic domain exclusively liberated diacetyl-chitobiose, whereas reactions with the C-terminal domain led to N-acetyl-chitooligosaccharides of various lengths. These results demonstrated that the N-terminal and C-terminal catalytic domains functioned as exo- and endochitinases, respectively. The biochemical results provide a physiological explanation for the presence of two catalytic domains with different specificity and suggest a cooperative function between the two on a single polypeptide in the degradation of chitin. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: endochitinase-like [Cicer arietinum] gi|502153653|ref|XP_004509418.1| | 4 | ||
– | Class Ia chitinase [Galega orientalis] gi|33414052|gb|AAP03088.1| | 4 | ||
– | Class II chitinase [Arachis hypogaea] gi|295291568|gb|ADF87393.1| | 4 | ||
– | PREDICTED: chitinase-like protein 2-like [Cicer arietinum] gi|502102342|ref|XP_004492044.1| | 4 | ||
– | Class Ia chitinase [Galega orientalis] gi|33414052|gb|AAP03088.1| | 4 | ||
– | PREDICTED: chitinase 10-like [Cicer arietinum] gi|502112896|ref|XP_004494478.1| | 4 | ||
– | PREDICTED: LOW QUALITY PROTEIN: endochitinase PR4-like [Cicer arietinum] gi|502081457|ref|XP_004486869.1| | 4 | ||
– | Endochitinase [Medicago truncatula] gi|357449033|ref|XP_003594792.1| | 4 | ||
– | Chitinase, putative; TAIR: AT1G05850.1 Chitinase family protein; Swiss-Prot: sp|Q9MA41|CTL1_ARATH Chitinase-like protein 1; TrEMBL-Plants: tr|I3S1Z0|I3S1Z0_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0530200 | 4 | ||
– | Chitinase; TAIR: AT3G12500.1 basic chitinase; Swiss-Prot: sp|P06215|CHIT_PHAVU Endochitinase; TrEMBL-Plants: tr|G7J999|G7J999_MEDTR Chitinase (Class I) / Hevein; Found in the gene: LotjaGi1g1v0556300 | 4 | ||
– | Chitinase; TAIR: AT4G01700.1 Chitinase family protein; Swiss-Prot: sp|Q5NB11|CHI10_ORYSJ Chitinase 10; TrEMBL-Plants: tr|C6TNB0|C6TNB0_SOYBN Putative uncharacterized protein; Found in the gene: LotjaGi1g1v0754300 | 4 | ||
– | Chitinase; TAIR: AT3G12500.1 basic chitinase; Swiss-Prot: sp|P06215|CHIT_PHAVU Endochitinase; TrEMBL-Plants: tr|Q7X9F5|Q7X9F5_9FABA Class Ia chitinase; Found in the gene: LotjaGi2g1v0287300 | 4 | ||
– | Chitinase; TAIR: AT3G54420.1 homolog of carrot EP3-3 chitinase; Swiss-Prot: sp|P27054|CHI4_PHAVU Endochitinase PR4; TrEMBL-Plants: tr|I3SA35|I3SA35_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0501600 | 4 | ||
– | Chitinase; TAIR: AT3G54420.1 homolog of carrot EP3-3 chitinase; Swiss-Prot: sp|P27054|CHI4_PHAVU Endochitinase PR4; TrEMBL-Plants: tr|I3SA35|I3SA35_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0501600 | 4 | ||
– | Chitinase; TAIR: AT3G54420.1 homolog of carrot EP3-3 chitinase; Swiss-Prot: sp|P27054|CHI4_PHAVU Endochitinase PR4; TrEMBL-Plants: tr|I3SA35|I3SA35_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0501600 | 4 | ||
– | Chitinase; TAIR: AT3G12500.1 basic chitinase; Swiss-Prot: sp|P36907|CHIX_PEA Endochitinase; TrEMBL-Plants: tr|I3RZQ4|I3RZQ4_LOTJA Uncharacterized protein; Found in the gene: LotjaGi4g1v0137500 | 4 | ||
– | Chitinase; TAIR: AT4G01700.1 Chitinase family protein; Swiss-Prot: sp|Q5NB11|CHI10_ORYSJ Chitinase 10; TrEMBL-Plants: tr|I3SJS9|I3SJS9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi5g1v0237600 | 4 | ||
– | Chitinase; TAIR: AT3G16920.2 chitinase-like protein; Swiss-Prot: sp|Q9LSP9|CTL2_ARATH Chitinase-like protein 2; TrEMBL-Plants: tr|G7IMU8|G7IMU8_MEDTR Chitinase; Found in the gene: LotjaGi6g1v0256500 | 4 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Biological process | Cell wall macromolecule catabolic process | 1 | 5.56 |