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IPR000089 is a Biotin/lipoyl attachment.
<p>The biotin/lipoyl attachment domain has a conserved lysine residue that binds biotin or lipoic acid. The 80 residues surrounding the biotinyl-binding lysine residue display some sequence similarity to that around the lipoyl-binding lysine residue. Biotin plays a catalytic role in some carboxyl transfer reactions and is covalently attached, via an amide bond, to a lysine residue in enzymes requiring this coenzyme [[cite:PUB00002740]]. E2 acyltransferases have an essential cofactor, lipoic acid, which is covalently bound via an amide linkage to a lysine group [[cite:PUB00000614]]. The lipoic acid cofactor is found in a variety of proteins that include, H-protein of the glycine cleavage system (GCS), mammalian and yeast pyruvate dehydrogenases and branched-chain 2-oxo acid dehydrogenase complex (BCOADC).</p> <p>The lipoyl domains of 2-oxo acid dehydrogenase multienzyme complexes and the biotinyl domains of biotin-dependent enzymes have been solved, which revealed that they have homologous structures consisting of a flattened 8-stranded -barrel with the target lysine positioned in comparable β-turns. Additional important residues for specificity have been identified, such as the conserved methionine flanking the target lysine that is essential for the recognition of the biotinyl domain by the biotinyl protein ligase [[cite:PUB00014190], [cite:PUB00005256]].</p>
This description is obtained from EB-eye REST.
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: acetyl-CoA carboxylase 1-like [Glycine max] gi|356508126|ref|XP_003522811.1| | 23 | ||
| – | PREDICTED: biotin carboxyl carrier protein of acetyl-CoA carboxylase 1, chloroplastic-like [Cicer arietinum] gi|502130085|ref|XP_004500525.1| | 9 | ||
| – | PREDICTED: biotin carboxyl carrier protein of acetyl-CoA carboxylase, chloroplastic-like [Glycine max] gi|356531878|ref|XP_003534503.1| | 16 | ||
| – | Biotin carboxyl carrier protein subunit [Glycine max] gi|351721795|ref|NP_001235174.1| | 15 | ||
| – | PREDICTED: biotin carboxyl carrier protein of acetyl-CoA carboxylase 2, chloroplastic-like [Glycine max] gi|356551156|ref|XP_003543944.1| | 15 | ||
| – | PREDICTED: dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex-like [Glycine max] gi|356498274|ref|XP_003517978.1| | 23 | ||
| – | PREDICTED: dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2, mitochondrial-like [Glycine max] gi|356501546|ref|XP_003519585.1| | 19 | ||
| – | PREDICTED: biotin carboxyl carrier protein of acetyl-CoA carboxylase 1, chloroplastic-like [Glycine max] gi|356550600|ref|XP_003543673.1| | 8 | ||
| – | PREDICTED: dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex-like [Glycine max] gi|356498274|ref|XP_003517978.1| | 18 | ||
| – | PREDICTED: glycine cleavage system H protein, mitochondrial-like [Cicer arietinum] gi|502143341|ref|XP_004505304.1| | 16 | ||
| – | PREDICTED: ent-kaurenoic acid oxidase 2-like [Glycine max] gi|356495547|ref|XP_003516638.1| | 19 | ||
| – | PREDICTED: lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial-like [Glycine max] gi|356564223|ref|XP_003550355.1| | 21 | ||
| – | PREDICTED: methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial-like isoform X2 [Cicer arietinum] gi|502115337|ref|XP_004495179.1| | 33 | ||
| – | PREDICTED: dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial-like [Glycine max] gi|356576335|ref|XP_003556288.1| | 22 | ||
| – | PREDICTED: dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial-like [Glycine max] gi|356576165|ref|XP_003556204.1| | 21 | ||
| – | Acetyltransferase component of pyruvate dehydrogenase complex; TAIR: AT4G26910.1 Dihydrolipoamide succinyltransferase; Swiss-Prot: sp|Q8H107|ODO2B_ARATH Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2, mitochondrial; TrEMBL-Plants: tr|A0A1J7GQS7|A0A1J7GQS7_LUPAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0026900 | 19 | ||
| – | Acetyl-CoA carboxylase; TAIR: AT1G36160.1 acetyl-CoA carboxylase 1; Swiss-Prot: sp|Q38970|ACC1_ARATH Acetyl-CoA carboxylase 1; TrEMBL-Plants: tr|A0A0R0K6S7|A0A0R0K6S7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0246300 | 46 | ||
| – | Acetyl-CoA carboxylase; TAIR: AT1G36160.1 acetyl-CoA carboxylase 1; Swiss-Prot: sp|Q38970|ACC1_ARATH Acetyl-CoA carboxylase 1; TrEMBL-Plants: tr|A0A0R0K6S7|A0A0R0K6S7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0246300 | 46 | ||
| – | Acetyl-CoA carboxylase; TAIR: AT1G36160.1 acetyl-CoA carboxylase 1; Swiss-Prot: sp|Q38970|ACC1_ARATH Acetyl-CoA carboxylase 1; TrEMBL-Plants: tr|I1JVH6|I1JVH6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0246300 | 46 | ||
| – | Acetyl-CoA carboxylase; TAIR: AT1G36160.1 acetyl-CoA carboxylase 1; Swiss-Prot: sp|Q38970|ACC1_ARATH Acetyl-CoA carboxylase 1; TrEMBL-Plants: tr|A0A0R0K6S7|A0A0R0K6S7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0246300 | 46 | ||
| – | Acetyl-CoA carboxylase; TAIR: AT1G36160.1 acetyl-CoA carboxylase 1; Swiss-Prot: sp|Q38970|ACC1_ARATH Acetyl-CoA carboxylase 1; TrEMBL-Plants: tr|A0A0R0K6S7|A0A0R0K6S7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0246300 | 32 | ||
| – | Biotin carboxyl carrier protein of acetyl-CoA carboxylase; TAIR: AT5G16390.1 chloroplastic acetylcoenzyme A carboxylase 1; Swiss-Prot: sp|Q42783|BCCP_SOYBN Biotin carboxyl carrier protein of acetyl-CoA carboxylase, chloroplastic; TrEMBL-Plants: tr|I3RZK7|I3RZK7_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0486900 | 16 | ||
| – | Biotin carboxyl carrier protein of acetyl-CoA carboxylase; TAIR: AT5G15530.1 biotin carboxyl carrier protein 2; Swiss-Prot: sp|Q9LLC1|BCCP2_ARATH Biotin carboxyl carrier protein of acetyl-CoA carboxylase 2, chloroplastic; TrEMBL-Plants: tr|I3SZG2|I3SZG2_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0510100 | 15 | ||
| – | Acetyltransferase component of pyruvate dehydrogenase complex; TAIR: AT3G52200.1 Dihydrolipoamide acetyltransferase, long form protein; Swiss-Prot: sp|Q0WQF7|ODP21_ARATH Dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial; TrEMBL-Plants: tr|I3SH23|I3SH23_LOTJA Acetyltransferase component of pyruvate dehydrogenase complex; Found in the gene: LotjaGi2g1v0157500 | 26 | ||
| – | Biotin carboxyl carrier protein of acetyl-CoA carboxylase; TAIR: AT5G15530.1 biotin carboxyl carrier protein 2; Swiss-Prot: sp|Q9LLC1|BCCP2_ARATH Biotin carboxyl carrier protein of acetyl-CoA carboxylase 2, chloroplastic; TrEMBL-Plants: tr|I3SWG0|I3SWG0_LOTJA Uncharacterized protein; Found in the gene: LotjaGi2g1v0265400 | 15 | ||
| – | Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; TAIR: AT3G25860.1 2-oxoacid dehydrogenases acyltransferase family protein; Swiss-Prot: sp|Q9SQI8|ODP24_ARATH Dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex, chloroplastic; TrEMBL-Plants: tr|I1J6K8|I1J6K8_SOYBN Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Found in the gene: LotjaGi2g1v0278200 | 25 | ||
| – | Glycine cleavage system h protein; TAIR: AT1G32470.1 Single hybrid motif superfamily protein; Swiss-Prot: sp|Q39732|GCSH_FLAAN Glycine cleavage system H protein, mitochondrial; TrEMBL-Plants: tr|I3SRF4|I3SRF4_LOTJA Uncharacterized protein; Found in the gene: LotjaGi2g1v0415700 | 16 | ||
| – | Biotin carboxyl carrier protein of acetyl-CoA carboxylase; TAIR: AT3G15690.2 Single hybrid motif superfamily protein; Swiss-Prot: sp|Q06881|BCCP_NOSS1 Biotin carboxyl carrier protein of acetyl-CoA carboxylase; TrEMBL-Plants: tr|I3T9E9|I3T9E9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0011700 | 8 | ||
| – | Acetyltransferase component of pyruvate dehydrogenase complex; TAIR: AT3G13930.1 Dihydrolipoamide acetyltransferase, long form protein; Swiss-Prot: sp|Q8RWN9|ODP22_ARATH Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial; TrEMBL-Plants: tr|A0A0B2S5Z1|A0A0B2S5Z1_GLYSO Acetyltransferase component of pyruvate dehydrogenase complex; Found in the gene: LotjaGi3g1v0419400 | 25 | ||
| – | Acetyltransferase component of pyruvate dehydrogenase complex; TAIR: AT3G13930.1 Dihydrolipoamide acetyltransferase, long form protein; Swiss-Prot: sp|Q8RWN9|ODP22_ARATH Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial; TrEMBL-Plants: tr|A0A0B2S5Z1|A0A0B2S5Z1_GLYSO Acetyltransferase component of pyruvate dehydrogenase complex; Found in the gene: LotjaGi3g1v0419400 | 25 | ||
| – | Acetyltransferase component of pyruvate dehydrogenase complex; TAIR: AT1G54220.1 Dihydrolipoamide acetyltransferase, long form protein; Swiss-Prot: sp|Q5M729|ODP23_ARATH Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial; TrEMBL-Plants: tr|A0A151T9E3|A0A151T9E3_CAJCA Acetyltransferase component of pyruvate dehydrogenase complex; Found in the gene: LotjaGi3g1v0419400 | 25 | ||
| – | Acetyltransferase component of pyruvate dehydrogenase complex; TAIR: AT1G54220.1 Dihydrolipoamide acetyltransferase, long form protein; Swiss-Prot: sp|Q5M729|ODP23_ARATH Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial; TrEMBL-Plants: tr|A0A151T9E3|A0A151T9E3_CAJCA Acetyltransferase component of pyruvate dehydrogenase complex; Found in the gene: LotjaGi3g1v0419400 | 25 | ||
| – | Acetyltransferase component of pyruvate dehydrogenase complex; TAIR: AT1G54220.1 Dihydrolipoamide acetyltransferase, long form protein; Swiss-Prot: sp|Q5M729|ODP23_ARATH Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial; TrEMBL-Plants: tr|A0A151T9E3|A0A151T9E3_CAJCA Acetyltransferase component of pyruvate dehydrogenase complex; Found in the gene: LotjaGi3g1v0419400 | 25 | ||
| – | Glycine cleavage system H family protein; TAIR: AT2G35120.1 Single hybrid motif superfamily protein; Swiss-Prot: sp|O82179|GCSH2_ARATH Glycine cleavage system H protein 2, mitochondrial; TrEMBL-Plants: tr|I3S7G1|I3S7G1_LOTJA Uncharacterized protein; Found in the gene: LotjaGi4g1v0083600 | 16 | ||
| – | Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; TAIR: AT3G06850.1 2-oxoacid dehydrogenases acyltransferase family protein; Swiss-Prot: sp|Q9M7Z1|ODB2_ARATH Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial; TrEMBL-Plants: tr|A0A072V2G4|A0A072V2G4_MEDTR Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Found in the gene: LotjaGi4g1v0338800 | 22 | ||
| – | Acetyl-CoA carboxylase, putative; TAIR: AT1G03090.2 methylcrotonyl-CoA carboxylase alpha chain; Swiss-Prot: sp|Q42777|MCCA_SOYBN Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; TrEMBL-Plants: tr|A0A151QXF8|A0A151QXF8_CAJCA Uncharacterized protein; Found in the gene: LotjaGi5g1v0170200 | 30 | ||
| – | Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; TAIR: AT1G34430.1 2-oxoacid dehydrogenases acyltransferase family protein; Swiss-Prot: sp|Q9C8P0|ODP25_ARATH Dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex, chloroplastic; TrEMBL-Plants: tr|A0A0S3SNP9|A0A0S3SNP9_PHAAN Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Found in the gene: LotjaGi5g1v0279000 | 23 | ||
| – | Biotin carboxyl carrier acetyl-CoA carboxylase; TAIR: AT3G56130.1 biotin/lipoyl attachment domain-containing protein; Swiss-Prot: sp|Q06881|BCCP_NOSS1 Biotin carboxyl carrier protein of acetyl-CoA carboxylase; TrEMBL-Plants: tr|I3SR75|I3SR75_LOTJA Uncharacterized protein; Found in the gene: LotjaGi6g1v0081500 | 9 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 2.63 |