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IPR000340 is a Dual specificity phosphatase, catalytic domain.
<p>This entry represents dual specificity protein-tyrosine phosphatases. Ser/Thr and Tyr dual specificity phosphatases are a group of enzymes with both Ser/Thr ([ec:3.1.3.16]) and tyrosine specific protein phosphatase ([ec:3.1.3.48]) activity able to remove both the serine/threonine or tyrosine-bound phosphate group from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. Dual specificity protein phosphatases (DSPs) regulate mitogenic signal transduction and control the cell cycle. The crystal structure of a human DSP, vaccinia H1-related phosphatase (or VHR), has been determined at 2.1 angstrom resolution [[cite:PUB00005219]]. A shallow active site pocket in VHR allows for the hydrolysis of phosphorylated serine, threonine, or tyrosine protein residues, whereas the deeper active site of protein tyrosine phosphatases (PTPs) restricts substrate specificity to only phosphotyrosine. Positively charged crevices near the active site may explain the enzyme's preference for substrates with two phosphorylated residues. The VHR structure defines a conserved structural scaffold for both DSPs and PTPs. A "recognition region" connecting helix alpha1 to strand beta1, may determine differences in substrate specificity between VHR, the PTPs, and other DSPs.</p> <p>These proteins may also have inactive phosphatase domains, and dependent on the domain composition this loss of catalytic activity has different effects on protein function. Inactive single domain phosphatases can still specifically bind substrates, and protect again dephosphorylation, while the inactive domains of tandem phosphatases can be further subdivided into two classes. Those which bind phosphorylated tyrosine residues may recruit multi-phosphorylated substrates for the adjacent active domains and are more conserved, while the other class have accumulated several variable amino acid substitutions and have a complete loss of tyrosine binding capability. The second class shows a release of evolutionary constraint for the sites around the catalytic centre, which emphasises a difference in function from the first group. There is a region of higher conservation common to both classes, suggesting a new regulatory centre [[cite:PUB00014502]].</p> <p>Protein tyrosine (pTyr) phosphorylation is a common post-translational modification which can create novel recognition motifs for protein interactions and cellular localisation, affect protein stability, and regulate enzyme activity. Consequently, maintaining an appropriate level of protein tyrosine phosphorylation is essential for many cellular functions. Tyrosine-specific protein phosphatases (PTPase; [ec:3.1.3.48]) catalyse the removal of a phosphate group attached to a tyrosine residue, using a cysteinyl-phosphate enzyme intermediate. These enzymes are key regulatory components in signal transduction pathways (such as the MAP kinase pathway) and cell cycle control, and are important in the control of cell growth, proliferation, differentiation and transformation [[cite:PUB00035793], [cite:PUB00035794]]. The PTP superfamily can be divided into four subfamilies [[cite:PUB00035795]]:</p> <ul> <li>(1) pTyr-specific phosphatases</li> <li>(2) dual specificity phosphatases (dTyr and dSer/dThr)</li> <li>(3) Cdc25 phosphatases (dTyr and/or dThr)</li> <li>(4) LMW (low molecular weight) phosphatases</li> </ul> <p>Based on their cellular localisation, PTPases are also classified as:</p> <ul> <li>Receptor-like, which are transmembrane receptors that contain PTPase domains [[cite:PUB00035796]]</li> <li>Non-receptor (intracellular) PTPases [[cite:PUB00035797]]</li> </ul> <p>All PTPases carry the highly conserved active site motif C(X)5R (PTP signature motif), employ a common catalytic mechanism, and share a similar core structure made of a central parallel β-sheet with flanking α-helices containing a β-loop-α-loop that encompasses the PTP signature motif [[cite:PUB00035798]]. Functional diversity between PTPases is endowed by regulatory domains and subunits.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
GO term | Namespace | Name | Definition | Relationships |
---|---|---|---|---|
Molecular function | Protein tyrosine/serine/threonine phosphatase activity | Catalysis of the reactions: protein serine + H2O = protein serine + phosphate; protein threonine phosphate + H2O = protein threonine + phosphate; and protein tyrosine phosphate + H2O = protein tyrosine + phosphate. | ||
Biological process | Dephosphorylation | The process of removing one or more phosphoric (ester or anhydride) residues from a molecule. |
Transcript | Name | Description | Predicted domains | Domain count |
---|---|---|---|---|
– | PREDICTED: mRNA-capping enzyme-like [Glycine max] gi|356568041|ref|XP_003552222.1| | 29 | ||
– | PREDICTED: laforin-like [Glycine max] gi|356516265|ref|XP_003526816.1| | 13 | ||
– | PREDICTED: dual specificity protein phosphatase PHS1-like [Cicer arietinum] gi|502133383|ref|XP_004501744.1| | 21 | ||
– | PREDICTED: protein-tyrosine-phosphatase MKP1-like [Cicer arietinum] gi|502102464|ref|XP_004492084.1| | 20 | ||
– | PREDICTED: phosphoglucan phosphatase LSF1, chloroplastic-like [Cicer arietinum] gi|502170387|ref|XP_004514819.1| | 19 | ||
– | PREDICTED: dual specificity protein phosphatase 12-like isoform X2 [Cicer arietinum] gi|502108122|ref|XP_004493392.1| | 16 | ||
– | PREDICTED: mRNA-capping enzyme-like [Glycine max] gi|356570562|ref|XP_003553454.1| | 20 | ||
– | PREDICTED: mRNA-capping enzyme-like [Glycine max] gi|356570562|ref|XP_003553454.1| | 26 | ||
– | PREDICTED: mRNA-capping enzyme-like [Glycine max] gi|356570562|ref|XP_003553454.1| | 16 | ||
– | Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN [Medicago truncatula] gi|357482767|ref|XP_003611670.1| | 15 | ||
– | PREDICTED: protein-tyrosine-phosphatase IBR5-like [Glycine max] gi|356501245|ref|XP_003519436.1| | 16 | ||
– | PREDICTED: mRNA-capping enzyme-like [Glycine max] gi|356504293|ref|XP_003520931.1| | 22 | ||
– | PREDICTED: mRNA-capping enzyme-like [Glycine max] gi|356496154|ref|XP_003516935.1| | 29 | ||
– | PREDICTED: protein-tyrosine phosphatase mitochondrial 1-like protein-like [Glycine max] gi|356510670|ref|XP_003524059.1| | 15 | ||
– | PREDICTED: dual specificity protein phosphatase 1-like isoform X1 [Cicer arietinum] gi|502151309|ref|XP_004508377.1| | 14 | ||
– | PREDICTED: dual specificity protein phosphatase 1-like isoform X2 [Cicer arietinum] gi|502151311|ref|XP_004508378.1| | 13 | ||
– | PREDICTED: dual specificity protein phosphatase PHS1-like [Cicer arietinum] gi|502139515|ref|XP_004503799.1| | 22 | ||
– | PREDICTED: protein-tyrosine phosphatase mitochondrial 1-like [Glycine max] gi|356523809|ref|XP_003530527.1| | 15 | ||
– | PREDICTED: phosphoglucan phosphatase DSP4, chloroplastic-like [Cicer arietinum] gi|502118212|ref|XP_004496155.1| | 18 | ||
– | Phosphotyrosine protein phosphatases superfamily protein [Theobroma cacao] gi|508721723|gb|EOY13620.1| | 16 | ||
– | PREDICTED: protein-tyrosine-phosphatase MKP1-like isoform X1 [Cicer arietinum] gi|502135593|ref|XP_004502386.1| | 20 | ||
– | PREDICTED: protein-tyrosine-phosphatase MKP1-like isoform X2 [Cicer arietinum] gi|502135596|ref|XP_004502387.1| | 20 | ||
– | Dual specificity protein phosphatase, putative; TAIR: AT2G04550.1 indole-3-butyric acid response 5; Swiss-Prot: sp|Q84JU4|IBR5_ARATH Protein-tyrosine-phosphatase IBR5; TrEMBL-Plants: tr|I1JIH0|I1JIH0_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0068800 | 16 | ||
– | Dual specificity protein phosphatase, putative; TAIR: AT3G10940.1 dual specificity protein phosphatase (DsPTP1) family protein; Swiss-Prot: sp|Q9SRK5|LSF2_ARATH Phosphoglucan phosphatase LSF2, chloroplastic; TrEMBL-Plants: tr|I3SHM7|I3SHM7_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0309000 | 13 | ||
– | Dual specificity phosphatase family protein; TAIR: AT5G23720.1 dual specificity protein phosphatase family protein; Swiss-Prot: sp|Q75QN6|DPHS1_ARATH Dual specificity protein phosphatase PHS1; TrEMBL-Plants: tr|A0A0S3RFM3|A0A0S3RFM3_PHAAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0334000 | 21 | ||
– | Dual specificity phosphatase family protein; TAIR: AT5G23720.3 dual specificity protein phosphatase family protein; Swiss-Prot: sp|Q75QN6|DPHS1_ARATH Dual specificity protein phosphatase PHS1; TrEMBL-Plants: tr|A0A151R609|A0A151R609_CAJCA Dual specificity protein phosphatase 3; Found in the gene: LotjaGi1g1v0334000 | 14 | ||
– | Dual specificity phosphatase; TAIR: AT3G55270.1 mitogen-activated protein kinase phosphatase 1; Swiss-Prot: sp|Q9C5S1|MKP1_ARATH Protein-tyrosine-phosphatase MKP1; TrEMBL-Plants: tr|A0A0L9U245|A0A0L9U245_PHAAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0533600 | 19 | ||
– | MRNA-capping enzyme; TAIR: AT3G09100.2 mRNA capping enzyme family protein; Swiss-Prot: sp|O55236|MCE1_MOUSE mRNA-capping enzyme; TrEMBL-Plants: tr|K7MTE7|K7MTE7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0572100 | 30 | ||
– | MRNA-capping enzyme; TAIR: AT3G09100.2 mRNA capping enzyme family protein; Swiss-Prot: sp|O55236|MCE1_MOUSE mRNA-capping enzyme; TrEMBL-Plants: tr|K7MTE7|K7MTE7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0572100 | 29 | ||
– | MRNA-capping enzyme; TAIR: AT3G09100.2 mRNA capping enzyme family protein; Swiss-Prot: sp|O55236|MCE1_MOUSE mRNA-capping enzyme; TrEMBL-Plants: tr|A0A0R0FB81|A0A0R0FB81_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0572100 | 29 | ||
– | MRNA-capping enzyme; TAIR: AT3G09100.2 mRNA capping enzyme family protein; Swiss-Prot: sp|O55236|MCE1_MOUSE mRNA-capping enzyme; TrEMBL-Plants: tr|K7MTE7|K7MTE7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0572100 | 29 | ||
– | Phosphoglucan phosphatase LSF1; TAIR: AT3G01510.1 like SEX4 1; Swiss-Prot: sp|F4J117|LSF1_ARATH Phosphoglucan phosphatase LSF1, chloroplastic; TrEMBL-Plants: tr|K7MWV8|K7MWV8_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0607500 | 22 | ||
– | Dual specificity protein phosphatase, putative; TAIR: AT4G18593.1 dual specificity protein phosphatase-like protein; Swiss-Prot: sp|Q9UNI6|DUS12_HUMAN Dual specificity protein phosphatase 12; TrEMBL-Plants: tr|Q2HSZ6|Q2HSZ6_MEDTR Dual specificity phosphatase domain protein; Found in the gene: LotjaGi1g1v0662200 | 15 | ||
– | MRNA-capping enzyme; TAIR: AT3G09100.2 mRNA capping enzyme family protein; Swiss-Prot: sp|O55236|MCE1_MOUSE mRNA-capping enzyme; TrEMBL-Plants: tr|A0A0B2SM84|A0A0B2SM84_GLYSO mRNA-capping enzyme; Found in the gene: LotjaGi1g1v0728900 | 27 | ||
– | Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; TAIR: AT3G19420.1 PTEN 2; Swiss-Prot: sp|Q9LT75|PTN2A_ARATH Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and protein-tyrosine-phosphatase PTEN2A; TrEMBL-Plants: tr|I1J9A2|I1J9A2_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0404800 | 16 | ||
– | MRNA-capping enzyme; TAIR: AT3G09100.2 mRNA capping enzyme family protein; Swiss-Prot: sp|O55236|MCE1_MOUSE mRNA-capping enzyme; TrEMBL-Plants: tr|K7KDH1|K7KDH1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0215700 | 23 | ||
– | MRNA-capping enzyme; TAIR: AT3G09100.2 mRNA capping enzyme family protein; Swiss-Prot: sp|O55236|MCE1_MOUSE mRNA-capping enzyme; TrEMBL-Plants: tr|K7K355|K7K355_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0215700 | 30 | ||
– | MRNA-capping enzyme; TAIR: AT3G09100.2 mRNA capping enzyme family protein; Swiss-Prot: sp|O55236|MCE1_MOUSE mRNA-capping enzyme; TrEMBL-Plants: tr|A0A151RAA8|A0A151RAA8_CAJCA mRNA-capping enzyme; Found in the gene: LotjaGi3g1v0215700 | 29 | ||
– | MRNA-capping enzyme; TAIR: AT3G09100.2 mRNA capping enzyme family protein; Swiss-Prot: sp|O55236|MCE1_MOUSE mRNA-capping enzyme; TrEMBL-Plants: tr|K7K355|K7K355_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0215700 | 30 | ||
– | Dual specificity protein phosphatase; TAIR: AT2G35680.1 Phosphotyrosine protein phosphatases superfamily protein; Swiss-Prot: sp|Q9ZQP1|DSP8_ARATH Putative dual specificity protein phosphatase DSP8; TrEMBL-Plants: tr|I1K0T6|I1K0T6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0200000 | 15 | ||
– | Dual specificity protein phosphatase; TAIR: AT3G23610.1 dual specificity protein phosphatase 1; Swiss-Prot: sp|Q9ZR37|DUS1_ARATH Dual specificity protein phosphatase 1; TrEMBL-Plants: tr|A0A072V237|A0A072V237_MEDTR Dual specificity protein phosphatase; Found in the gene: LotjaGi4g1v0352400 | 13 | ||
– | Dual specificity protein phosphatase, putative; TAIR: AT3G55270.1 mitogen-activated protein kinase phosphatase 1; Swiss-Prot: sp|Q9C5S1|MKP1_ARATH Protein-tyrosine-phosphatase MKP1; TrEMBL-Plants: tr|A0A1J7GIS4|A0A1J7GIS4_LUPAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0392500 | 19 | ||
– | Dual specificity phosphatase family protein; TAIR: AT5G23720.1 dual specificity protein phosphatase family protein; Swiss-Prot: sp|Q75QN6|DPHS1_ARATH Dual specificity protein phosphatase PHS1; TrEMBL-Plants: tr|A0A1J7IUU8|A0A1J7IUU8_LUPAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0421000 | 14 | ||
– | Dual specificity phosphatase family protein; TAIR: AT5G23720.1 dual specificity protein phosphatase family protein; Swiss-Prot: sp|Q75QN6|DPHS1_ARATH Dual specificity protein phosphatase PHS1; TrEMBL-Plants: tr|A0A072TU75|A0A072TU75_MEDTR Dual specificity phosphatase family protein; Found in the gene: LotjaGi4g1v0421000 | 22 | ||
– | Dual specificity phosphatase family protein; TAIR: AT5G23720.1 dual specificity protein phosphatase family protein; Swiss-Prot: sp|Q75QN6|DPHS1_ARATH Dual specificity protein phosphatase PHS1; TrEMBL-Plants: tr|A0A0B2Q765|A0A0B2Q765_GLYSO Dual specificity protein phosphatase 1; Found in the gene: LotjaGi4g1v0421000 | 21 | ||
– | Dual specificity phosphatase family protein; TAIR: AT5G23720.1 dual specificity protein phosphatase family protein; Swiss-Prot: sp|Q75QN6|DPHS1_ARATH Dual specificity protein phosphatase PHS1; TrEMBL-Plants: tr|K7KS75|K7KS75_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0421000 | 22 | ||
– | Dual specificity phosphatase family protein; TAIR: AT5G23720.3 dual specificity protein phosphatase family protein; Swiss-Prot: sp|Q75QN6|DPHS1_ARATH Dual specificity protein phosphatase PHS1; TrEMBL-Plants: tr|A0A0B2Q765|A0A0B2Q765_GLYSO Dual specificity protein phosphatase 1; Found in the gene: LotjaGi4g1v0421000 | 21 | ||
– | Dual specificity phosphatase family protein; TAIR: AT5G23720.1 dual specificity protein phosphatase family protein; Swiss-Prot: sp|Q75QN6|DPHS1_ARATH Dual specificity protein phosphatase PHS1; TrEMBL-Plants: tr|A0A1J7IUU8|A0A1J7IUU8_LUPAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0421000 | 14 | ||
– | Dual specificity phosphatase; TAIR: AT2G35680.1 Phosphotyrosine protein phosphatases superfamily protein; Swiss-Prot: sp|Q9ZQP1|DSP8_ARATH Putative dual specificity protein phosphatase DSP8; TrEMBL-Plants: tr|I1KPV3|I1KPV3_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0443600 | 15 | ||
– | Dual specificity phosphatase; TAIR: AT2G35680.1 Phosphotyrosine protein phosphatases superfamily protein; Swiss-Prot: sp|Q9ZQP1|DSP8_ARATH Putative dual specificity protein phosphatase DSP8; TrEMBL-Plants: tr|G7L7D1|G7L7D1_MEDTR Dual specificity phosphatase domain protein; Found in the gene: LotjaGi4g1v0443600 | 15 | ||
– | Dual specificity phosphatase; TAIR: AT2G35680.1 Phosphotyrosine protein phosphatases superfamily protein; Swiss-Prot: sp|Q9ZQP1|DSP8_ARATH Putative dual specificity protein phosphatase DSP8; TrEMBL-Plants: tr|I1KPV3|I1KPV3_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0443600 | 15 | ||
– | Dual specificity phosphatase; TAIR: AT3G52180.1 dual specificity protein phosphatase (DsPTP1) family protein; Swiss-Prot: sp|G4LTX4|DSP4_CASSA Phosphoglucan phosphatase DSP4, amyloplastic; TrEMBL-Plants: tr|I3SIE2|I3SIE2_LOTJA Uncharacterized protein; Found in the gene: LotjaGi5g1v0264500 | 21 | ||
– | Dual specificity protein phosphatase family protein; TAIR: AT5G56610.1 Phosphotyrosine protein phosphatases superfamily protein; Swiss-Prot: sp|P76093|YNBD_ECOLI Uncharacterized protein YnbD; TrEMBL-Plants: tr|I3T491|I3T491_LOTJA Uncharacterized protein; Found in the gene: LotjaGi5g1v0344700 | 16 | ||
– | Dual specificity protein phosphatase, putative; TAIR: AT3G55270.1 mitogen-activated protein kinase phosphatase 1; Swiss-Prot: sp|Q9C5S1|MKP1_ARATH Protein-tyrosine-phosphatase MKP1; TrEMBL-Plants: tr|V7D1Y7|V7D1Y7_PHAVU Uncharacterized protein; Found in the gene: LotjaGi6g1v0095400 | 19 | ||
– | Dual specificity protein phosphatase, putative; TAIR: AT3G55270.1 mitogen-activated protein kinase phosphatase 1; Swiss-Prot: sp|Q9C5S1|MKP1_ARATH Protein-tyrosine-phosphatase MKP1; TrEMBL-Plants: tr|V7D1Y7|V7D1Y7_PHAVU Uncharacterized protein; Found in the gene: LotjaGi6g1v0095400 | 19 | ||
– | Dual specificity protein phosphatase, putative; TAIR: AT3G55270.1 mitogen-activated protein kinase phosphatase 1; Swiss-Prot: sp|Q9C5S1|MKP1_ARATH Protein-tyrosine-phosphatase MKP1; TrEMBL-Plants: tr|V7D1Y7|V7D1Y7_PHAVU Uncharacterized protein; Found in the gene: LotjaGi6g1v0095400 | 19 |
A list of co-occurring predicted domains within the L. japonicus gene space:
Predicted domain | Source | Observations | Saturation (%) |
---|---|---|---|
mobidb-lite | MobiDBLite | 1 | 1.79 |