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IPR000169

Description

IPR000169 is a Cysteine peptidase, cysteine active site.

<p>The sequences around the three active site residues are well conserved. This entry represents the cysteine active site. The catalytic triad consists of this entry, [interpro:IPR025660] and [interpro:IPR025661]. This catalytic triad detects mainly proteases of the C1 family, including papain and several cathepsins.</p> <p>Thiol (cysteine) proteases (EC 3.4.22.-) [[cite:PUB00000685]] are a family of proteolytic enzymes which contain an active site cysteine. Catalysis proceeds through a thioester intermediate and is facilitated by a nearby histidine side chain; an asparagine completes the essential catalytic triad.</p> <p>Cysteine peptidases have characteristic molecular topologies, which can be seen not only in their three-dimensional structures, but commonly also in the two-dimensional structures. These are peptidases in which the nucleophile is the sulphydryl group of a cysteine residue. Cysteine proteases are divided into clans (proteins which are evolutionary related), and further sub-divided into families, on the basis of the architecture of their catalytic dyad (cysteine-histidene) or triad [[cite:PUB00011704]].</p> <p>Modification of the catalytic triad, especially of its first amino acid (cysteine), has been postulated as a suitable target for a chemical modulation of enzyme function. This is the case for silicateins, where the cysteine residue has been replaced by a serine [[cite:PUB00057831]]. Silicateins represent a group of enzymes possessing bi-functional activity; in addition to the silica-condensing activity, they possess a proteolytic (cathepsin-like) activity [[cite:PUB00057832]].</p> <p>A cysteine peptidase is a proteolytic enzyme that hydrolyses a peptide bond using the thiol group of a cysteine residue as a nucleophile. Hydrolysis involves usually a catalytic triad consisting of the thiol group of the cysteine, the imidazolium ring of a histidine, and a third residue, usually asparagine or aspartic acid, to orientate and activate the imidazolium ring. In only one family of cysteine peptidases, is the role of the general base assigned to a residue other than a histidine: in peptidases from family C89 (acid ceramidase) an arginine is the general base. Cysteine peptidases can be grouped into fourteen different clans, with members of each clan possessing a tertiary fold unique to the clan. Four clans of cysteine peptidases share structural similarities with serine and threonine peptidases and asparagine lyases. From sequence similarities, cysteine peptidases can be clustered into over 80 different families [[cite:PUB00011704]]. Clans CF, CM, CN, CO, CP and PD contain only one family.</p> <p>Cysteine peptidases are often active at acidic pH and are therefore confined to acidic environments, such as the animal lysosome or plant vacuole. Cysteine peptidases can be endopeptidases, aminopeptidases, carboxypeptidases, dipeptidyl-peptidases or omega-peptidases. They are inhibited by thiol chelators such as iodoacetate, iodoacetic acid,<i>N</i>-ethylmaleimide or<i>p</i>-chloromercuribenzoate.</p> <p>Clan CA includes proteins with a papain-like fold. There is a catalytic triad which occurs in the order: Cys/His/Asn (or Asp). A fourth residue, usually Gln, is important for stabilising the acyl intermediate that forms during catalysis, and this precedes the active site Cys. The fold consists of two subdomains with the active site between them. One subdomain consists of a bundle of helices, with the catalytic Cys at the end of one of them, and the other subdomain is a β-barrel with the active site His and Asn (or Asp). There are over thirty families in the clan, and tertiary structures have been solved for members of most of these. Peptidases in clan CA are usually sensitive to the small molecule inhibitor E64, which is ineffective against peptidases from other clans of cysteine peptidases [[cite:PUB00076953]].</p> <p>Clan CD includes proteins with a caspase-like fold. Proteins in the clan have an α/β/α sandwich structure. There is a catalytic dyad which occurs in the order His/Cys. The active site His occurs in a His-Gly motif and the active site Cys occurs in an Ala-Cys motif; both motifs are preceded by a block of hydrophobic residues [[cite:PUB00020025]]. Specificity is predominantly directed towards residues that occupy the S1 binding pocket, so that caspases cleave aspartyl bonds, legumains cleave asparaginyl bonds, and gingipains cleave lysyl or arginyl bonds.</p> <p>Clan CE includes proteins with an adenain-like fold. The fold consists of two subdomains with the active site between them. One domain is a bundle of helices, and the other a β-barrel. The subdomains are in the opposite order to those found in peptidases from clan CA, and this is reflected in the order of active site residues: His/Asn/Gln/Cys. This has prompted speculation that proteins in clans CA and CE are related, and that members of one clan are derived from a circular permutation of the structure of the other.</p> <p>Clan CL includes proteins with a sortase B-like fold. Peptidases in the clan hydrolyse and transfer bacterial cell wall peptides. The fold shows a closed β-barrel decorated with helices with the active site at one end of the barrel [[cite:PUB00030423]]. The active site consists of a His/Cys catalytic dyad.</p> <p>Cysteine peptidases with a chymotrypsin-like fold are included in clan PA, which also includes serine peptidases. Cysteine peptidases that are N-terminal nucleophile hydrolases are included in clan PB. Cysteine peptidases with a tertiary structure similar to that of the serine-type aspartyl dipeptidase are included in clan PC. Cysteine peptidases with an intein-like fold are included in clan PD, which also includes asparagine lyases.</p>

This description is obtained from EB-eye REST.

Associated GO terms

Unable to find any GO terms for the transcript with the identifier.

Associated Lotus transcripts 62

Transcript Name Description Predicted domains Domain count
PREDICTED: cathepsin B-like [Glycine max] gi|356572872|ref|XP_003554589.1| 24
Senescence-associated gene 12 [Theobroma cacao] gi|508702326|gb|EOX94222.1| 24
Calpain-like protein [Medicago truncatula] gi|357519297|ref|XP_003629937.1| 12
PREDICTED: LOW QUALITY PROTEIN: xylem cysteine proteinase 1-like [Cicer arietinum] gi|502078248|ref|XP_004485885.1| 26
PREDICTED: KDEL-tailed cysteine endopeptidase CEP2-like [Cicer arietinum] gi|502143938|ref|XP_004505522.1| 14
PREDICTED: cysteine proteinase 15A [Glycine max] gi|356564325|ref|XP_003550405.1| 15
Cysteine proteinase precursor [Glycine max] gi|351721126|ref|NP_001237199.1| 27
Cysteine proteinase [Lotus japonicus] gi|144905116|dbj|BAF56430.1| 27
PREDICTED: cysteine proteinase RD21a-like [Glycine max] gi|356563584|ref|XP_003550041.1| 17
PREDICTED: oryzain alpha chain-like [Cicer arietinum] gi|502131381|ref|XP_004500967.1| 32
PREDICTED: cysteine proteinase RD19a-like [Glycine max] gi|356509908|ref|XP_003523684.1| 27
PREDICTED: xylem cysteine proteinase 2-like [Glycine max] gi|356517188|ref|XP_003527271.1| 25
Cysteine proteinase [Lotus japonicus] gi|144905108|dbj|BAF56428.1| 19
Cysteine proteinase [Lotus japonicus] gi|144905116|dbj|BAF56430.1| 16
Cysteine proteinase [Lotus japonicus] gi|144905108|dbj|BAF56428.1| 19
Cysteine proteinase [Lotus japonicus] gi|144905108|dbj|BAF56428.1| 20
Cysteine proteinase [Lotus japonicus] gi|144905116|dbj|BAF56430.1| 19
PREDICTED: cathepsin B-like [Glycine max] gi|356505709|ref|XP_003521632.1| 14
CM0216.510.nc [Lotus japonicus] gi|164605519|dbj|BAF98585.1| 26
CM0216.500.nc [Lotus japonicus] gi|164605518|dbj|BAF98584.1| 25
Cysteine proteinase [Lotus japonicus] gi|409190991|gb|AFV30165.1| 26
Cysteine proteinase [Lotus japonicus] gi|144905104|dbj|BAF56427.1| 26
PREDICTED: vignain-like [Glycine max] gi|356563155|ref|XP_003549830.1| 27
PREDICTED: cysteine proteinase RD21a-like [Cicer arietinum] gi|502125689|ref|XP_004499026.1| 30
PREDICTED: cysteine proteinase RD21a-like isoform X1 [Cicer arietinum] gi|502120445|ref|XP_004496965.1| 30
Cysteine proteinase [Medicago truncatula] gi|357446993|ref|XP_003593772.1| 26
Cysteine proteinase [Medicago truncatula] gi|357446993|ref|XP_003593772.1| 16
PREDICTED: LOW QUALITY PROTEIN: xylem cysteine proteinase 1-like [Cicer arietinum] gi|502078248|ref|XP_004485885.1| 27
Cysteine proteinase; TAIR: AT5G50260.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|P25803|CYSEP_PHAVU Vignain; TrEMBL-Plants: tr|I1KC88|I1KC88_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0230500 27
Cysteine protease family protein; TAIR: AT1G09850.1 xylem bark cysteine peptidase 3; Swiss-Prot: sp|Q9LT78|RD21C_ARATH Probable cysteine protease RD21C; TrEMBL-Plants: tr|A0A1J7IIQ9|A0A1J7IIQ9_LUPAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0423400 34
Cysteine proteinase; TAIR: AT4G39090.1 Papain family cysteine protease; Swiss-Prot: sp|P25804|CYSP_PEA Cysteine proteinase 15A; TrEMBL-Plants: tr|A0A151RQM7|A0A151RQM7_CAJCA Cysteine proteinase RD19a; Found in the gene: LotjaGi1g1v0424400 27
Cysteine proteinase; TAIR: AT4G35350.1 xylem cysteine peptidase 1; Swiss-Prot: sp|O65493|XCP1_ARATH Cysteine protease XCP1; TrEMBL-Plants: tr|I1K796|I1K796_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0569300 26
Cathepsin B-like cysteine protease; TAIR: AT1G02305.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|P07858|CATB_HUMAN Cathepsin B; TrEMBL-Plants: tr|I3SCV5|I3SCV5_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0753100 15
Cathepsin B-like cysteine protease; TAIR: AT1G02305.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|Q4R5M2|CATB_MACFA Cathepsin B; TrEMBL-Plants: tr|I3SCV5|I3SCV5_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0753100 23
Cathepsin B-like cysteine protease; TAIR: AT1G02305.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|Q4R5M2|CATB_MACFA Cathepsin B; TrEMBL-Plants: tr|I3SCV5|I3SCV5_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0753100 23
Cathepsin B-like cysteine protease; TAIR: AT1G02305.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|P07858|CATB_HUMAN Cathepsin B; TrEMBL-Plants: tr|I3SCV5|I3SCV5_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0753100 15
Cysteine protease; TAIR: AT1G06260.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|Q9FJ47|SAG12_ARATH Senescence-specific cysteine protease SAG12; TrEMBL-Plants: tr|A0A1J7H619|A0A1J7H619_LUPAN Uncharacterized protein; Found in the gene: LotjaGi3g1v0245200 28
Cysteine protease; TAIR: AT1G06260.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|Q9FJ47|SAG12_ARATH Senescence-specific cysteine protease SAG12; TrEMBL-Plants: tr|G8A001|G8A001_MEDTR Papain family cysteine protease; Found in the gene: LotjaGi3g1v0245300 27
Cysteine protease; TAIR: AT5G45890.1 senescence-associated gene 12; Swiss-Prot: sp|A2XQE8|SAG39_ORYSI Senescence-specific cysteine protease SAG39; TrEMBL-Plants: tr|K7K0Y1|K7K0Y1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0248000 27
Cysteine protease, putative; TAIR: AT5G45890.1 senescence-associated gene 12; Swiss-Prot: sp|A2XQE8|SAG39_ORYSI Senescence-specific cysteine protease SAG39; TrEMBL-Plants: tr|K4MQ82|K4MQ82_LOTJA Cysteine proteinase; Found in the gene: LotjaGi3g1v0249300 27
Cysteine protease, putative; TAIR: AT5G45890.1 senescence-associated gene 12; Swiss-Prot: sp|A2XQE8|SAG39_ORYSI Senescence-specific cysteine protease SAG39; TrEMBL-Plants: tr|A4PIZ3|A4PIZ3_LOTJA Cysteine proteinase; Found in the gene: LotjaGi3g1v0254300 20
Cysteine protease; TAIR: AT5G45890.1 senescence-associated gene 12; Swiss-Prot: sp|A2XQE8|SAG39_ORYSI Senescence-specific cysteine protease SAG39; TrEMBL-Plants: tr|A4PIZ2|A4PIZ2_LOTJA Cysteine proteinase; Found in the gene: LotjaGi3g1v0489300 27
Cysteine protease, putative; TAIR: AT5G45890.1 senescence-associated gene 12; Swiss-Prot: sp|A2XQE8|SAG39_ORYSI Senescence-specific cysteine protease SAG39; TrEMBL-Plants: tr|A4PIZ2|A4PIZ2_LOTJA Cysteine proteinase; Found in the gene: LotjaGi3g1v0489400 26
Cysteine proteinase; TAIR: AT4G39090.1 Papain family cysteine protease; Swiss-Prot: sp|P43296|RD19A_ARATH Cysteine protease RD19A; TrEMBL-Plants: tr|B0BL96|B0BL96_LOTJA CM0216.510.nc protein; Found in the gene: LotjaGi3g1v0508700 27
Cysteine proteinase; TAIR: AT4G39090.1 Papain family cysteine protease; Swiss-Prot: sp|P43296|RD19A_ARATH Cysteine protease RD19A; TrEMBL-Plants: tr|Q70I35|Q70I35_LOTJA Papain-like cysteine proteinase-like protein 2; Found in the gene: LotjaGi3g1v0512500 26
Cysteine proteinase; TAIR: AT4G39090.1 Papain family cysteine protease; Swiss-Prot: sp|P43296|RD19A_ARATH Cysteine protease RD19A; TrEMBL-Plants: tr|B0BL95|B0BL95_LOTJA CM0216.500.nc protein; Found in the gene: LotjaGi3g1v0512700 26
Cysteine protease; TAIR: AT1G47128.1 Granulin repeat cysteine protease family protein; Swiss-Prot: sp|P43297|RD21A_ARATH Cysteine proteinase RD21A; TrEMBL-Plants: tr|I1K268|I1K268_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0101100 27
Cysteine protease, putative; TAIR: AT5G50260.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|P25803|CYSEP_PHAVU Vignain; TrEMBL-Plants: tr|A0A072UQV2|A0A072UQV2_MEDTR KDEL-tailed cysteine endopeptidase CEP1; Found in the gene: LotjaGi4g1v0226100 27
Calpain, putative; TAIR: AT1G55350.1 calpain-type cysteine protease family; Swiss-Prot: sp|Q8RVL2|DEK1_ARATH Calpain-type cysteine protease DEK1; TrEMBL-Plants: tr|A0A151UC24|A0A151UC24_CAJCA Calpain-9; Found in the gene: LotjaGi4g1v0240000_LC 9
Calpain, putative; TAIR: AT1G55350.1 calpain-type cysteine protease family; Swiss-Prot: sp|Q8RVL2|DEK1_ARATH Calpain-type cysteine protease DEK1; TrEMBL-Plants: tr|A0A151UC24|A0A151UC24_CAJCA Calpain-9; Found in the gene: LotjaGi4g1v0240000_LC 9
Cysteine protease; TAIR: AT3G45310.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|Q8RWQ9|ALEUL_ARATH Thiol protease aleurain-like; TrEMBL-Plants: tr|I3SZ47|I3SZ47_LOTJA Uncharacterized protein; Found in the gene: LotjaGi4g1v0324600 27
Calpain, putative; TAIR: AT1G55350.1 calpain-type cysteine protease family; Swiss-Prot: sp|Q8RVL2|DEK1_ARATH Calpain-type cysteine protease DEK1; TrEMBL-Plants: tr|V7CN28|V7CN28_PHAVU Uncharacterized protein; Found in the gene: LotjaGi4g1v0386800 31
Calpain, putative; TAIR: AT1G55350.1 calpain-type cysteine protease family; Swiss-Prot: sp|Q8RVL2|DEK1_ARATH Calpain-type cysteine protease DEK1; TrEMBL-Plants: tr|V7CN28|V7CN28_PHAVU Uncharacterized protein; Found in the gene: LotjaGi4g1v0386800 31
Calpain, putative; TAIR: AT1G55350.1 calpain-type cysteine protease family; Swiss-Prot: sp|Q8RVL2|DEK1_ARATH Calpain-type cysteine protease DEK1; TrEMBL-Plants: tr|V7CN28|V7CN28_PHAVU Uncharacterized protein; Found in the gene: LotjaGi4g1v0386800 31
Cysteine proteinase; TAIR: AT4G39090.1 Papain family cysteine protease; Swiss-Prot: sp|P25804|CYSP_PEA Cysteine proteinase 15A; TrEMBL-Plants: tr|I1MC68|I1MC68_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0010700 27
Cysteine proteinase; TAIR: AT1G47128.1 Granulin repeat cysteine protease family protein; Swiss-Prot: sp|P43297|RD21A_ARATH Cysteine proteinase RD21A; TrEMBL-Plants: tr|A0A1J7IKX0|A0A1J7IKX0_LUPAN Uncharacterized protein; Found in the gene: LotjaGi5g1v0030300 33
Cysteine proteinase; TAIR: AT3G54940.2 Papain family cysteine protease; Swiss-Prot: sp|Q8VYS0|RD19D_ARATH Probable cysteine protease RD19D; TrEMBL-Plants: tr|A0A072VPA6|A0A072VPA6_MEDTR Papain family cysteine protease; Found in the gene: LotjaGi5g1v0269900 25
Cysteine protease, putative; TAIR: AT5G43060.1 Granulin repeat cysteine protease family protein; Swiss-Prot: sp|Q9FMH8|RD21B_ARATH Probable cysteine protease RD21B; TrEMBL-Plants: tr|A0A1J7HJC8|A0A1J7HJC8_LUPAN Uncharacterized protein; Found in the gene: LotjaGi5g1v0329500 33
Cysteine protease; TAIR: AT1G09850.1 xylem bark cysteine peptidase 3; Swiss-Prot: sp|P25776|ORYA_ORYSJ Oryzain alpha chain; TrEMBL-Plants: tr|A0A151S6D2|A0A151S6D2_CAJCA Cysteine proteinase RD21a; Found in the gene: LotjaGi6g1v0322400 35
Cysteine protease; TAIR: AT1G20850.1 xylem cysteine peptidase 2; Swiss-Prot: sp|P22895|P34_SOYBN P34 probable thiol protease; TrEMBL-Plants: tr|G7ILJ3|G7ILJ3_MEDTR Papain family cysteine protease; Found in the gene: LotjaGi6g1v0323700 26
Cysteine protease; TAIR: AT3G49340.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|Q9FJ47|SAG12_ARATH Senescence-specific cysteine protease SAG12; TrEMBL-Plants: tr|I3S8V9|I3S8V9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi6g1v0324000 26
Cysteine protease; TAIR: AT2G34080.1 Cysteine proteinases superfamily protein; Swiss-Prot: sp|Q9FJ47|SAG12_ARATH Senescence-specific cysteine protease SAG12; TrEMBL-Plants: tr|I3S8V9|I3S8V9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi6g1v0324100 27

Co-occuring domains 1

A list of co-occurring predicted domains within the L. japonicus gene space:

Predicted domain Source Observations Saturation (%)
mobidb-lite MobiDBLite 1 1.61